Applications of the comet assay in human biomonitoring

Issues in Toxicology

Volumn , Issue , 2009, Pages 201-226

  Collins, Andrew R ^a   Dusinska, Maria ^b,c  

^a UNIVERSITY OF OSLO   (Norway)

^b NORWEGIAN INSTITUTE FOR AIR RESEARCH   (Norway)

^c SLOVAK MEDICAL UNIVERSITY   (Slovakia)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 84898730035     PISSN: 17577179     EISSN: 17577187     Source Type: Book Series    
DOI: None     Document Type: Article

References (62)

1. J. Angerer, U. Ewers and M. Wilhelm, Human biomonitoring: state-of-theart, Int. J. Hyg. Environ. Health, 2007, 210, 201-28.
2. D. A. Bennett and M. D. Waters, Applying biomarker research, Environ. Health Persp., 2005, 108, 907-910.
3. A. Collins, M. Dušinská, M. Franklin, M. Somorovská, H. Petrovská, S. Duthie, L. Fillion, M. Panayiotidis, K. Rašlová and N. Vaughan, Comet assay in human biomonitoring studies: reliability, validation, and applications, Environ. Molec. Mutagen., 1997, 30, 139-146.
4. P. Moller, L. E. Knudsen, S. Loft and H. Wallin, The Comet assay as a rapid test in biomonitoring occupational exposure to DNA-damaging agents and effect of confounding factors, Cancer Epidem. Biomarkers Prev., 2000, 9, 1005-1015.
5. F. Faust, F. Kassie, S. Knasmüller, R. H. Boedecker, M. Mann and V. Mersch-Sundermann, The use of the alkaline Comet assay with lymphocytes in human biomonitoring studies, Mutat. Res., 2004, 566, 209-229.
6. P. Moller, The alkaline Comet assay: towards validation in biomonitoring of DNA damaging exposures, Basic Clin. Pharmacol. Toxicol., 2006, 98, 336-45.
7. R. R. Tice, E. Agurell, A. Anderson, B. Burlinson, A. Hartmann, H. Kobayashi, Y. Miyamae, E. Rojas, J.-C. Y. Ryu and F. Sasaki, Single cell gel/Comet assay: guidelines for in vitro and in vivo genetic toxicology testing, Environ. Molec. Mutagen., 2000, 35, 206-221.
8. M. Dušinská, R. Fábry, E. Szabová, M. Somorovská, H. Petrovská and A. R. Collins, Occupational exposure to mutagens; monitoring of rubber factory workers, Mutat. Res., 1997, 379(Suppl. S-XVI), 161-162.
9. ESCODD, Measurement of DNA oxidation in human cells by chromatographic and enzymic methods, Free Rad. Biol. Med., 2003, 34, 1089-1099.
10. A. R. Collins, The Comet assay for DNA damage and repair: principles, applications, and limitations, Mol Biotechnol., 2004, 26, 249-261.
11. G. Speit, P. Schütz, I. Bonzheim, K. Trenz and H. Hoffmann, Sensitivity of the FPG protein towards alkylation damage in the Comet assay, Toxicol. Lett., 2004, 15, 151-158.
12. C. C. Smith, M. R. O'Donovan and E. A. Martin, hOGG1 recognizes oxidative damage using the Comet assay with greater specificity than FPG or ENDOIII, Mutagenesis, 2006, 21, 185-190.
13. K. G. Berdal, R. F. Johansen and E. Seeberg, Release of normal bases from intact DNA by a native DNA repair enzyme, EMBO. J., 1998, 17, 363-367.
14. R. J. Albertini, D. Anderson, G. R. Douglas, L. Hagmar, K. Hemminki, F. Merlo, A. T. Natarajan, H. Norppa, D. E. G. Shuker, R. Tice, M. D. Waters and A. Aitio, IPCS guidelines for the monitoring of genotoxic effects of carcinogens in humans, Mutat. Res., 2000, 463, 111-172.
15. M. N. Bates, J. W. Hamilton, J. S. La Kind, P. Langenberg, M. O'Malley and W. Snodgrass, Workgroup report: biomonitoring study design, interpretation, and communication - lessons learned and path forward, Environ. Health Perspect, 2005, 113, 1615-1621.
16. W. W. Au, Usefulness of biomarkers in population studies: from exposure to susceptibility and to prediction of cancer, Int. J. Hyg. Environ. Health, 2007, 210, 239-246.
17. ESCODD, Comparative analysis of baseline 8-oxo-7, 8-dihydroguanine in mammalian cell DNA, by different methods in different laboratories: an approach to consensus, Carcinogenesis, 2002, 23, 2129-2133..
18. D. P. Lovell and T. Omori, Statistical issues in the use of the comet Assay, Mutagenesis, 2008, 23, 171-182.
19. A. R. Collins, Oxidative DNA damage biomakers: A need for quality control, In: Critical Reviews of oxidative stress and ageing, eds. R. G. Cutler and H. Rodriguez, World Scientific, New Jersey, 2003, p. 469.
20. G. Speit and A. Hartmann, The Comet assay a sensitive genotoxicity test for the detection of DNA damage and repair, Methods Mol. Biol., 2006, 2314, 275-286.
21. P. Møller, Assessment of reference values for DNA damage detected by the Comet assay in human blood cell DNA, Mutat. Res., 2006, 612, 84-104.
22. E. Rojas, M. Valverde, M. Sordo and P. Ostrosky-Wegman, DNA damage in exfoliated buccal cells of smokers assessed by the single-cell gel electrophoresis assay, Mutat. Res., 1996, 370, 115-20.
23. D. Pinhal, A. M. Gontijo, V. A. Reyes and D. M. Salvadori, Viable human buccal mucosa cells do not yield typical nucleoids: impacts on the singlecell gel electrophoresis/Comet assay, Environ. Molec. Mutagen., 2006, 47, 117-126.
24. K. Eren, N. Ozmeriç and S. Sarda S, Monitoring of buccal epithelial cells by alkaline Comet assay (single-cell gel electrophoresis technique) in cytogenetic evaluation of chlorhexidine, Clin. Oral Investig., 2002, 6, 150-154.
25. T. I. Fortoul, M. Valverde, M. C. López, M. R. Avila-Costa, M. C. Avila-Casado, P. Mussali-Galante, A. Gonzalez-Villalva, E. Rojas and P. Ostrosky-Shejet, Genotoxic differences by sex in nasal epithelium and blood leukocytes in subjects residing in a highly polluted area, Environ. Res., 2004, 94 243-248.
26. Y. T. Szeto, I. F. Benzie, A. R. Collins, S. W. Choi, C. Y. Cheng, C. M. Yow and M. M. Tse, A buccal cell model Comet assay: development and evaluation for human biomonitoring and nutritional studies, Mutat. Res., 2005, 578, 371-81.
27. E. Rojas, M. Valverde, M. C. Lopez, I. Naufal, I. Sanchez, P. Bizarro, I. Lopez, T. I. Fortoul and P. Ostrosky-Wegman, Evaluation of DNA damage in exfoliated tear duct epithelial cells from individuals exposed to air pollution assessed by single-cell gel electrophoresis assay, Mutat. Res., 2000, 468, 11-17.
28. B. L. Pool-Zobel, I. Dornacher, R. Lambertz, M. Knoll and H. K. Seitz, Genetic damage and repair in human rectal cells for biomonitoring: sex differences, effects of alcohol exposure, and susceptibilities in comparison to peripheral blood lymphocytes, Mutat. Res., 2004, 551, 127-34.
29. C. M. Hughes, S. E. Lewis, V. J. McKelvey-Martin and W. Thompson, A comparison of baseline and induced DNA damage in human spermatozoa from fertile and infertile men, using a modified Comet assay, Molec. Hum. Reprod., 1996, 2, 613-619.
30. M. Sergerie, G. Bleau, R. Teulé, M. Daudin and L. Bujan, Sperm DNA integrity as diagnosis and prognosis element of male fertility, Gynecol. Obstet. Fertil., 2005, 33, 89-101.
31. L. Migliore, R. Colognato, A. Naccarati and E. Bergamaschi, Relationship between genotoxicity biomarkers in somatic and germ cells: findings from a biomonitoring study, Mutagenesis, 2006, 21, 149-52.
32. I. Hininger, A. Chollat-Namy, S. Sauvaigo, M. Osman, H. Faure, J. Cadet, A. Favier and A. M. Roussel, Assessment of DNA damage by Comet assay on frozen total blood: method and evaluation in smokers and non-smokers, Mutat. Res., 2004, 558, 75-80.
33. C. Betti, T. Davini, L. Giannessi, N. Loprieno and R. Barale, Comparative studies by Comet test and SCE analysis in human lymphocytes from 200 healthy subjects, Mutat. Res., 1995, 343, 201-207.
34. M. Dusinska and A. R. Collins, The Comet assay in human biomonitoring: gene-environment interactions, Mutagenesis, 2008, 23, 191-205.
35. D. Anderson, T. W. Yu, M. M. Dobrzyńska, G. Ribas and R. Marcos, Effects in the Comet assay of storage conditions on human blood, Teratog. Carcinog. Mutagen., 1997, 17, 115-125.
36. M. Dušinská, B. Vallová, M. Ursínyová, V. Hladíková, B. Smolková, L. Wsólová, K. Rašlová and A. R. Collins, DNA damage and antioxidants: fluctuation through the year in a central European population group, Food Chem. Toxicol., 2002, 40, 1119-1123.
37. B. Smolková, M. Dušinská, K. Rašlová, G. McNeill, V. Spustová, P. Blazí́ček, A. Horská and A. Collins, Effects of seasonal variation in diet on markers of oxidative damage to lipids and DNA in defined population groups in Slovakia, Mutat. Res., 2004, 551, 135-144.
38. P. Møller, H. Wallin, E. Holst and L. E. Knudsen, Sunlight induced DNA damage in human mononuclear cells, FASEB J., 2002, 16, 45-53.
39. L. Giovannelli, C. Saieva, G. Masala, G. Testa, S. Salvini, V. Pitozzi, E. Riboli, P. Dolara and D. Palli, Nutritional and lifestyle determinants of DNA oxidative damage: a study in a Mediterranean population, Carcinogenesis, 2002, 23, 1483-1489.
40. P. Møller, Genotoxicity of environmental agents assessed by the alkaline Comet assay, Basic Clin. Pharmacol. Toxicol., 2005, 96(Suppl. 1), 1-42.
41. L. Hagmar, S. Bonassi, U. Strömberg, A. Brøgger, L. E. Knudsen, H. Norppa and C. Reuterwall and the European Study Group on Cytogenetic Biomarkers and Health, Chromosome aberrations in lymphocytes predict human cancer: A report from the European study group on cytogenetic biomarkers and health (ESCH), Cancer Res., 1998, 58, 4117-4121.
42. S. Bonassi, A. Znaor, M. Ceppi, C. Lando, W. P. Chang and N. Holland, et al., An increased micronucleus frequency in peripheral blood lymphocytes predicts the risk of cancer in humans, Carcinogenesis, 2007, 28, 625-631.
43. L. Rison, P. Møller and S. Loft, Oxidative stress-induced DNA damage by particulate air pollution, Mutat. Res., 2005, 592, 119-137.
44. M. Dušinská, K. Rašlová, J. Lietava, M. Somorovská, H. Petrovská, P. Dobríková and A. R. Collins, Elevated DNA damage in lymphocytes from ankylosing spondylitis, diabetes and hyperlipidemic patients, In Natural Antioxidants and Anticarcinogens in Nutrition, Health and Disease, ed. J. T. Kumpulainen and J. T. Salonen, Royal Society of Chemistry, Cambridge, 1999, 433-436.
45. M. Panayiotidis and A. R. Collins, Ex vivo assessment of lymphocyte antioxidant status using the Comet assay, Free Rad. Res., 1997, 27, 533-537.
46. A. Cebulska-Wasilewska, A. Wiecheć, A. Panek, B. Binková, R. J. Srám and P. B. Farmer, Influence of environmental exposure to PAHs on the susceptibility of lymphocytes to DNA-damage induction and on their repair capacity, Mutat. Res., 2005, 30, 73-81.
47. A. Cebulska-Wasilewska, I. Pawzyk, A. Panek, A. Wiecheć, I. Kalina, T. Popov, T. Georgieva and P. B. Farmer, Exposure to environmental polycyclic aromatic hydrocarbons: influences on cellular susceptibility to DNA damage (sampling Kosice and Sofia), Mutat. Res., 2007, 620, 145-154.
48. P. Vodicka, R. Kumar, R. Stetina, L. Musak, P. Soucek, V. Haufroid, M. Sasiadek, L. Vodickova, A. Naccarati, J. Sedikova, S. Sanyal, M. Kuricova, V. Brsiak, H. Norppa, J. Buchancova and K. Hemminki, Markers of individual susceptibility and DNA repair rate in workers exposed to xenobiotics in a tyre plant, Environ. Mol. Mutagen., 2004, 44, 283-292.
49. P. Vodicka, J. Tuimala, R. Stetina, R. Kumar, P. Manini, A. Naccarati, L. Maestri, L. Vodickova, M. Kuricova, H. Jarventaus, Z. Majvaldova, A. Hirvonen, M. Imbriani, A. Mutti, L. Migliore, H. Norppa and K. Hemminki, Cytogenetic markers, DNA single-strand breaks, urinary metabolites, and DNA repair rates in styrene-exposed lamination workers, Environ. Health Persp., 2004, 112, 867-871.
50. P. Aka, R. Mateuca, J.-P. Buchet, H. Thierens and M. Kirsch-Volders, Are genetic polymorphisms in Ogg1, XRCC1 and XRCC3 genes predictive for the DNA-strand break repair phenotype and genotoxicity in workers exposed to low dose ionising radiation? Mutat. Res., 2004, 556, 169-181.
51. A. R. Collins, M. Dušinská, E. Horváthová, E. Munro, M. Savio and R. Štetina, Inter-individual differences in repair of DNA base oxidation, measured in vitro with the Comet assay, Mutagenesis, 2001, 16, 297-301.
52. S. A. S. Langie, A. M. Knaapen, K. J. J. Brauers, D. van Berlo, F.-J. van Schooten and R. W. L. Godschalk, Development and validation of a modified Comet assay to phenotypically assess nucleotide excision repair, Mutagenesis, 2006, 21, 153-158.
53. I. Gaivao, A. Piasek, A. Brevik, S. Shaposhnikov and A. R. Collins, Comet assay-based methods for measuring DNA repair in vitro; estimates of inter- and intra-individual variation, Cell Biol. Toxicol., 2009, 25, 45-52.
54. M. Dušinská, A. R. Collins, A. Kazimírová, M. Barančoková, V. Harrington, K. Volkovová, M. Staruchová, A. Horská, L. Wsólová, A. Kočan, J. Petrík, M. Machata, B. Ratcliffe and S. Kyrtopoulos, Genotoxic effects of asbestos in humans, Mutat. Res., 2004, 553, 91-102.
55. M. Dušinská, A. Kazimírová, M. Barančoková, A. Horská, K. Burghardtova ́, K. Volkovová, M. Staruchová, L. Wsólová and A. R. Collins, Does occupational exposure to mineral fibres cause DNA or chromosome damage? Mutat. Res., 2004, 553, 103-110.
56. M. Dušinská, Z. Dzupinková, L. Wsólová , V. Harrington and A. R. Collins, Possible involvement of XPA in repair of oxidative DNA damage, deduced from analysis of damage, repair and genotype in a human population study, Mutagenesis, 2006, 21, 205-211.
57. A. R. Collins, V. L. Dobson, M. Dušinská, G. Kennedy and R. Štětina, The Comet assay: what can it really tell us? Mutat. Res., 1997, 375, 183-193.
58. G. Ahnström and K. Erixon, Measurement of strand breaks by alkaline denaturation and hydroxyapatite chromatography, in DNA Repair. A Laboratory Manual of Research Procedures, eds. E. C. Friedberg and P. C. Hanawalt, Marcel Dekker, New York, 1981, 403-18.
59. A. R. Collins, A. A. Oscoz, G. Brunborg, I. Gaivão, L. Giovannelli, M. Kruszewski, C. C. Smith and Rudolf Stetina, The Comet assay: topical issues, Mutagenesis, 23, 143-151.
60. F. Kassie, W. Parzefall and S. Knasmüller, Single-cell gel electrophoresis assay: a new technique for human biomonitoring studies, Mutat. Res., 2000, 463, 13-31.
61. F. Faust, F. Kassie, S. Knasmüller, S. Kevekordes and V. Mersch-Sundermann, Use of primary blood cells for the assessment of exposure to occupational genotoxicants in human biomonitoring studies, Toxicology, 2004, 198, 341-50.
62. U. Plappert, K. Raddatz, S. Roth and T. M. Fliedner, DNA-damage detection in man after radiation exposure - the Comet assay - its possible application for human biomonitoring, Stem Cells., 1995, 13(Suppl 1), 215-22.