Converting aspartase into a β-amino acid lyase by cluster screening

ChemCatChem

Volumn 6, Issue 4, 2014, Pages 965-968

  Vogel, Andreas ^a   Schmiedel, Ramona ^a   Hofmann, Ute ^a,d   Gruber, Karl ^b   Zangger, Klaus ^c  

^a c LEcta GmbH   (Germany)

^b UNIVERSITY OF GRAZ   (Austria)

^c Institute of Chemistry/Organic and Bioorganic Chemistry   (Austria)

^d UNIVERSITY OF APPLIED SCIENCES JENA   (Germany)

Author keywords

amino acids; aspartase; cluster screening; enzyme engineering; mutagenesis

Indexed keywords

BACTERIOLOGY; BIOCHEMICAL ENGINEERING; ENZYMES; MUTAGENESIS;

AMINO ACID SYNTHESIS; ASPARTASE; ENZYME ENGINEERING; HIGH-THROUGHPUT SCREENING; NATURAL SUBSTRATES; SCREENING STRATEGY; SUBSTRATE BINDING; SYNTHETIC ROUTES;

AMINO ACIDS;

EID: 84898619469     PISSN: 18673880     EISSN: 18673899     Source Type: Journal    
DOI: 10.1002/cctc.201300986     Document Type: Article

References (29)

1. M. M. Heberling, B. Wu, S. Bartsch, D. B. Janssen, Curr. Opin. Chem. Biol. 2013, 17, 250-260.
2. N. J. Turner, Curr. Opin. Chem. Biol. 2011, 15, 234-240.
3. Y. Asano, Y. Kato, C. Levy, P. Baker, D. Rice, 2004, 22, DOI:.
4. M. de Villiers, V. Puthan Veetil, H. Raj, J. de Villiers, G. J. Poelarends, ACS Chem. Biol. 2012, 7, 1618-1628.
5. G. Fibriansah, V. P. Veetil, G. J. Poelarends, A.-M. W. H. Thunnissen, Biochemistry 2011, 50, 6053-6062.
6. K. L. Morley, R. J. Kazlauskas, Trends Biotechnol. 2005, 23, 231-237.
7. M. T. Reetz, M. Bocola, J. D. Carballeira, D. Zha, A. Vogel, Angew. Chem. Int. Ed. 2005, 44, 4192-4196
8. Angew. Chem. 2005, 117, 4264-4268.
9. M. D. Toscano, K. J. Woycechowsky, D. Hilvert, Angew. Chem. Int. Ed. 2007, 46, 3212-3236
10. Angew. Chem. 2007, 119, 3274-3300.
11. R. E. Viola, Adv. Enzymol. RAMB 2000, 74, 295-341.
12. Y. Asano, I. Kira, K. Yokozeki, Biomol. Eng. 2005, 22, 95-101.
13. Y. Kawata, K. Tamura, M. Kawamura, K. Ikei, T. Mizobata, J. Nagai, M. Fujita, S. Yano, M. Tokushige, N. Yumoto, Eur. J. Biochem. 2000, 267, 1847-1857.
14. T. Fujii, H. Sakai, Y. Kawata, Y. Hata, J. Mol. Biol. 2003, 328, 635-654.
15. W. M. Patrick, A. E. Firth, Biomol. Eng. 2005, 22, 105-112.
16. M. T. Reetz, D. Kahakeaw, R. Lohmer, ChemBioChem 2008, 9, 1797-1804.
17. T. Greiner-Stöffele, M. Struhalla, Method from the Selection of Biomolecules from Biomolecule Variant Libraries, WO2005040376, 2003.
18. K. M. Polizzi, M. Parikh, C. U. Spencer, I. Matsumura, J. H. Lee, M. J. Realff, A. S. Bommarius, Biotechnol. Prog. 2006, 22, 961-967.
19. Y. Kawata, K. Tamura, S. Yano, T. Mizobata, J. Nagai, N. Esaki, K. Soda, M. Tokushige, N. Yumoto, Arch. Biochem. Biophys. 1999, 366, 40-46.
20. U. T. Bornscheuer, G. W. Huisman, R. J. Kazlauskas, S. Lutz, J. C. Moore, K. Robins, Nature 2012, 485, 185-194.
21. A. Vogel, D. Schwarze, T. Greiner-Stöffele, Nitrilases, WO2012048865, 2011.
22. A. Vogel, R. Schmiedel, S. Köpke, Method For The Alcohol Dehydrogenase Reduction Of A Secosteroid Derivative, WO2013102619, 2012.
23. J.-K. Weng, J. P. Noel, Cold Spring Harbor Symp. Quant. Biol. 2012, 77, 309-320.
24. J.-K. Weng, R. N. Philippe, J. P. Noel, Science 2012, 336, 1667-1670.
25. B. Wu, W. Szymański, G. G. Wybenga, M. M. Heberling, S. Bartsch, S. de Wildeman, G. J. Poelarends, B. L. Feringa, B. W. Dijkstra, D. B. Janssen, Angew. Chem. Int. Ed. 2012, 51, 482-486
26. Angew. Chem. 2012, 124, 497-501.
27. F. W. Studier, Protein Expression Purif. 2005, 41, 207-234.
28. T. Greiner-Stöffele, C. Feller, M. Struhalla, Process for Generating a Variant Library of DNA Sequences, WO2009146892, 2009.
29. R. Hanczkõ, A. Jámbor, A. Perl, I. Molnár-Perl, J. Chromatogr. A 2007, 1163, 25-42.