Engineering production of functional scfv antibody in E. coli by co-expressing the molecule chaperone skp

Frontiers in Cellular and Infection Microbiology

Volumn 4, Issue NOV, 2013, Pages

  Wang, Rongzhi ^a   Xiang, Shuangshuang ^a   Feng, Youjun ^b   Srinivas, Swaminath ^b   Zhang, Yonghui ^a   Lin, Mingshen ^c   Wang, Shihua ^a  

^a FUJIAN AGRICULTURE AND FORESTRY UNIVERSITY   (China)

^b UNIVERSITY OF ILLINOIS AT URBANA CHAMPAIGN   (United States)

^c TA INSTRUMENTS   (United States)

Author keywords

Co expression; Production; ScFv; Solubility; Vibrio parahaemolyticus

Indexed keywords

CHAPERONE; CHAPERONE SKP; IMMUNOGLOBULIN LIGHT CHAIN; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY; UNCLASSIFIED DRUG; DNA BINDING PROTEIN; ESCHERICHIA COLI PROTEIN; PROTEIN BINDING; SKP PROTEIN, E COLI;

AFFINITY CHROMATOGRAPHY; ANTIGEN EXPRESSION; ANTIGEN PURIFICATION; ARTICLE; CHEMOLUMINESCENCE; ENZYME LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; EXPRESSION VECTOR; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN ENGINEERING; PROTEIN EXPRESSION; WESTERN BLOTTING; BIOSYNTHESIS; GENE EXPRESSION; GENE VECTOR; GENETIC ENGINEERING; GENETICS; HUMAN; METABOLISM; PROCEDURES; SOLUBILITY; CO-EXPRESSION; METHODOLOGY; PRODUCTION; PROTEIN BINDING; SCFV; VIBRIO PARAHAEMOLYTICUS;

DNA-BINDING PROTEINS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME-LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GENE EXPRESSION; GENETIC ENGINEERING; GENETIC VECTORS; HUMANS; MOLECULAR CHAPERONES; PROTEIN BINDING; SINGLE-CHAIN ANTIBODIES; SOLUBILITY;

CO-EXPRESSION; PRODUCTION; SCFV; SOLUBILITY; VIBRIO PARAHAEMOLYTICUS;

EID: 84897923580     PISSN: None     EISSN: 22352988     Source Type: Journal    
DOI: 10.3389/fcimb.2013.00072     Document Type: Article

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