Overproduction of anti-Tn antibody MLS128 single-chain Fv fragment in Escherichia coli cytoplasm using a novel pCold-PDI vector

Protein Expression and Purification

Volumn 82, Issue 1, 2012, Pages 197-204

  Subedi, Ganesh P ^a,b   Satoh, Tadashi ^a,f   Hanashima, Shinya ^a   Ikeda, Akemi ^a   Nakada, Hiroshi ^c   Sato, Reiko ^d   Mizuno, Mamoru ^d   Yuasa, Noriyuki ^e   Fujita Yamaguchi, Yoko ^e   Yamaguchi, Yoshiki ^a,b  

^a RIKEN   (Japan)

^b TOKYO MEDICAL AND DENTAL UNIVERSITY   (Japan)

^c KYOTO SANGYO UNIVERSITY   (Japan)

^d NOGUCHI INSTITUTE   (Japan)

^e TOKAI UNIVERSITY   (Japan)

^f NAGOYA CITY UNIVERSITY   (Japan)

Author keywords

Disulfide bond; Escherichia coli; Protein disulfide isomerase; Protein expression; Single chain variable fragment

Indexed keywords

CARBOHYDRATE; HYBRID PROTEIN; PROTEIN DISULFIDE ISOMERASE; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY; THIOREDOXIN; TN ANTIGEN; TUMOR ANTIGEN;

ARTICLE; ESCHERICHIA COLI; GENE EXPRESSION; GENE VECTOR; GENETICS; HUMAN; IMMUNOLOGY; ISOLATION AND PURIFICATION; METHODOLOGY; MOLECULAR CLONING;

ANTIGENS, TUMOR-ASSOCIATED, CARBOHYDRATE; CARBOHYDRATES; CLONING, MOLECULAR; ESCHERICHIA COLI; GENE EXPRESSION; GENETIC VECTORS; HUMANS; PROTEIN DISULFIDE-ISOMERASES; RECOMBINANT FUSION PROTEINS; SINGLE-CHAIN ANTIBODIES; THIOREDOXINS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 84856105895     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2011.12.010     Document Type: Article

References (42)

1. T.T. Hansel, H. Kropshofer, T. Singer, J.A. Mitchell, and A.J. George The safety and side effects of monoclonal antibodies Nat Rev Drug Discov 9 2010 325 338
2. J.M. Thornton Disulphide bridges in globular proteins J Mol Biol 151 1981 261 287
3. R.E. Bird, and B.W. Walker Single chain antibody variable regions Trends Biotechnol 9 1991 132 137 (Pubitemid 121003095)
4. M. Kudou, D. Ejima, H. Sato, R. Yumioka, T. Arakawa, and K. Tsumoto Refolding single-chain antibody (scFv) using lauroyl-L-glutamate as a solubilization detergent and arginine as a refolding additive Protein Exp Purif 77 2011 68 74
5. K.K. Fursova, A.G. Laman, B.S. Melnik, G.V. Semisotnov, P. Kopylov, N.V. Kiseleva, V.A. Nesmeyanov, and F.A. Brovko Refolding of scFv mini-antibodies using size-exclusion chromatography via arginine solution layer J Chromatogr B 877 2009 2045 2051
6. M. Liu, X. Wang, C. Yin, Z. Zhang, Q. Lin, Y. Zhen, and H. Huang One-step on-column purification and refolding of a single-chain variable fragment (scFv) antibody against tumour necrosis factor α Biotechnol Appl Biochem 43 2006 137 145 (Pubitemid 43344555)
7. D. Esposito, and D.K. Chatterjee Enhancement of soluble protein expression through the use of fusion tags Curr Opin Biotechnol 17 2006 353 358 (Pubitemid 44163452)
8. K. Hayashi, and C. Kojima Efficient protein production method for NMR using soluble protein tags with cold shock expression vector J Biomol NMR 48 2010 147 155
9. D.S. Waugh Making the most of affinity tags Trends Biotechnol 23 2005 316 320 (Pubitemid 40726273)
10. P. Jurado, V. de Lorenzo, and L.A. Fernández Thioredoxin fusions increase folding of single chain Fv antibodies in the cytoplasm of Escherichia coli: evidence that chaperone activity is the prime effect of thioredoxin J Mol Biol 357 2006 49 61 (Pubitemid 43339313)
11. R. Kern, A. Malki, A. Holmgren, and G. Richarme Chaperone properties of Escherichia coli thioredoxin and thioredoxin reductase Biochem J 371 2003 965 972 (Pubitemid 36578895)
12. H.C. Hawkins, E.C. Blackburn, and R.B. Freedman Comparison of the activities of protein disulphide-isomerase and thioredoxin in catalysing disulphide isomerization in a protein substrate Biochem J 275 Pt 2 1991 349 353
13. H. Sonoda, Y. Kumada, T. Katsuda, and H. Yamaji Functional expression of single-chain Fv antibody in the cytoplasm of Escherichia coli by thioredoxin fusion and co-expression of molecular chaperones Protein Exp Purif 70 2010 248 253
14. T. Yasukawa, C. Kanei-Ishii, T. Maekawa, J. Fujimoto, T. Yamamoto, and S. Ishii Increase of solubility of foreign proteins in Escherichia coli by coproduction of the bacterial thioredoxin J Biol Chem 270 1995 25328 25331
15. A.A. McCarthy, P.W. Haebel, A. Törrönen, V. Rybin, E.N. Baker, and P. Metcalf Crystal structure of the protein disulfide bond isomerase, DsbC, from Escherichia coli Nat Struct Biol 7 2000 196 199 (Pubitemid 30140763)
16. A. Rietsch, D. Belin, N. Martin, and J. Beckwith An in vivo pathway for disulfide bond isomerization in Escherichia coli Proc Natl Acad Sci USA 93 1996 13048 13053 (Pubitemid 26382767)
17. H. Cai, C.C. Wang, and C.L. Tsou Chaperone-like activity of protein disulfide-isomerase in the refolding of a protein with no disulfide bonds J Biol Chem 269 1994 24550 24552 (Pubitemid 24311703)
18. J.L. Song, and C.C. Wang Chaperone-like activity of protein disulfide-isomerase in the refolding of rhodanese Eur J Biochem 231 1995 312 316
19. G. Qing, L.C. Ma, A. Khorchid, G.V. Swapna, T.K. Mal, M.M. Takayama, B. Xia, S. Phadtare, H. Ke, T. Acton, G.T. Montelione, M. Ikura, and M. Inouye Cold-shock induced high-yield protein production in Escherichia coli Nat Biotechnol 22 2004 877 882 (Pubitemid 38886608)
20. T. Rathnayaka, M. Tawa, S. Sohya, M. Yohda, Y. Kuroda, Biophysical characterization of highly active recombinant Gaussia luciferase expressed in Escherichia coli, Biochim Biophys Acta 1804 (2010) 1902-1907.
21. X. Hu, L. O'Hara, S. White, E. Magner, M. Kane, and J.G. Wall Optimisation of production of a domoic acid-binding scFv antibody fragment in Escherichia coli using molecular chaperones and functional immobilisation on a mesoporous silicate support Protein Exp Purif 52 2007 194 201 (Pubitemid 46038745)
22. K. Hayashi, and C. Kojima pCold-GST vector: a novel cold-shock vector containing GST tag for soluble protein production Protein Exp Purif 62 2008 120 127
23. H. Nakada, Y. Numata, M. Inoue, N. Tanaka, H. Kitagawa, I. Funakoshi, S. Fukui, and I. Yamashina Elucidation of an essential structure recognized by an anti-GalNAcα-Ser(Thr) monoclonal antibody (MLS 128) J Biol Chem 266 1991 12402 12405 (Pubitemid 21907107)
24. N. Morita, Y. Yajima, H. Asanuma, H. Nakada, and Y. Fujita-Yamaguchi Inhibition of cancer cell growth by anti-Tn monoclonal antibody MLS128 Biosci Trends 3 2009 32 37
25. N. Yuasa, H. Ogawa, T. Koizumi, K. Tsukamoto, A. Matsumoto-Takasaki, H. Asanuma, H. Nakada, Y. Fujita-Yamaguchi, Construction and expression of anti-Tn-antigen-specific single chain antibody genes from hybridoma producing MLS128 monoclonal antibody, J Biochem (in press).
26. A. Matsumoto-Takasaki, S. Hanashima, A. Aoki, N. Yuasa, H. Ogawa, R. Sato, H. Kawakami, M. Mizuno, H. Nakada, Y. Yamaguchi, Y. Fujita-Yamaguchi, Surface plasmon resonance and NMR analyses of anti Tn-antigen MLS128 monoclonal antibody binding to two or three consecutive Tn-antigen clusters, J Biochem (2011), doi:10.1093/jb/mvr138.
27. E. Kaiser, R.L. Colescott, C.D. Bossinger, and P.I. Cook Color test for detection of free terminal amino groups in the solid-phase synthesis of peptides Anal Biochem 34 1970 595 598
28. J.L. Markley, A. Bax, Y. Arata, C.W. Hilbers, R. Kaptein, B.D. Sykes, P.E. Wright, and K. Wüthrich Recommendations for the presentation of NMR structures of proteins and nucleic acids (IUPAC Recommendations 1998) Pure Appl Chem 70 1998 117 142 (Pubitemid 128484457)
29. T. Kajino, C. Ohto, M. Muramatsu, S. Obata, S. Udaka, Y. Yamada, and H. Takahashi A protein disulfide isomerase gene fusion expression system that increases the extracellular productivity of Bacillus brevis Appl Environ Microbiol 66 2000 638 642 (Pubitemid 30082752)
30. Y. Liu, T.J. Zhao, Y.B. Yan, and H.M. Zhou Increase of soluble expression in Escherichia coli cytoplasm by a protein disulfide isomerase gene fusion system Protein Expr Purif 44 2005 155 161 (Pubitemid 41565564)
31. Z. Zhang, Z.H. Li, F. Wang, M. Fang, C.C. Yin, Z.Y. Zhou, Q. Lin, and H.L. Huang Overexpression of DsbC and DsbG markedly improves soluble and functional expression of single-chain Fv antibodies in Escherichia coli Protein Expr Purif 26 2002 218 228 (Pubitemid 35326539)
32. Y. Yao, Y. Zhou, and C. Wang Both the isomerase and chaperone activities of protein disulfide isomerase are required for the reactivation of reduced and denatured acidic phospholipase A2 EMBO J 16 1997 651 658 (Pubitemid 27067793)
33. J.E. Chambers, T.J. Tavender, O.B. Oka, S. Warwood, D. Knight, and N.J. Bulleid The reduction potential of the active site disulfides of human protein disulfide isomerase limits oxidation of the enzyme by Ero1α J Biol Chem 285 2010 29200 29207
34. A. Zapun, D. Missiakas, S. Raina, and T.E. Creighton Structural functional characterization of DsbC, a protein involved in disulfide bond formation in Escherichia coli Biochemistry 34 1995 5075 5089
35. G. Krause, J. Lundström, J.L. Barea, C. Pueyo de la Cuesta, and A. Holmgren Mimicking the active site of protein disulfide-isomerase by substitution of proline 34 in Escherichia coli thioredoxin J Biol Chem 266 1991 9494 9500 (Pubitemid 21906684)
36. P.H. Bessette, F. Aslund, J. Beckwith, and G. Georgiou Efficient folding of proteins with multiple disulfide bonds in the Escherichia coli cytoplasm Proc Natl Acad Sci USA 96 1999 13703 13708
37. Y. Shirano, and D. Shibata Low temperature cultivation of Escherichia coli carrying a rice lipoxygenase L-2 cDNA produces a soluble and active enzyme at a high level FEBS Lett 271 1990 128 130 (Pubitemid 20336221)
38. C.H. Schein Solubility as a function of protein structure and solvent components Nat Biotechnol 8 1990 308 317
39. E. Osinaga, S. Bay, D. Tello, A. Babino, O. Pritsch, K. Assemat, D. Cantacuzene, H. Nakada, and P. Alzari Analysis of the fine specificity of Tn-binding proteins using synthetic glycopeptide epitopes and a biosensor based on surface plasmon resonance spectroscopy FEBS Lett 469 2000 24 28 (Pubitemid 30119862)
40. Y. Shinohara, Y. Hasegawa, H. Kaku, and N. Shibuya Elucidation of the mechanism enhancing the avidity of lectin with oligosaccharides on the solid phase surface Glycobiology 7 1997 1201 1208 (Pubitemid 28015809)
41. K. Tsumoto, Y. Ueda, K. Maenaka, K. Watanabe, K. Ogasahara, K. Yutani, and I. Kumagai Contribution to antibody-antigen interaction of structurally perturbed antigenic residues upon antibody binding J Biol Chem 269 1994 28777 28782 (Pubitemid 24366717)
42. T.N. Bhat, G.A. Bentley, G. Boulot, M.I. Greene, D. Tello, W. Dall'Acqua, H. Souchon, F.P. Schwarz, R.A. Mariuzza, and R.J. Poljak Bound water molecules and conformational stabilization help mediate an antigen-antibody association Proc Natl Acad Sci USA 91 1994 1089 1093 (Pubitemid 24048514)