Construction of a single-chain variable-fragment antibody against the superantigen staphylococcal enterotoxin B

Applied and Environmental Microbiology

Volumn 76, Issue 24, 2010, Pages 8184-8191

  Singh, Pawan Kumar ^a   Agrawal, Ranu ^a   Kamboj, Dev Vrat ^a   Gupta, Garima ^a   Boopathi, M ^a   Goel, Ajay Kumar ^a   Singh, Lokendra ^a,b  

^a DEFENCE RESEARCH AND DEVELOPMENT ESTABLISHMENT   (India)

^b Defence R D Organization   (India)

Author keywords

[No Author keywords available]

Indexed keywords

AFFINITY CONSTANTS; ANTIBODY GENES; BACTERIAL SYSTEMS; BIOLOGICAL WARFARE AGENTS; BIOPANNING; CLONE ENCODING; ENZYME LINKED IMMUNOSORBENT ASSAY; FOOD POISONING; FOOD-BORNE ILLNESS; HIGH AFFINITY; IMMUNODETECTION; PHAGE DISPLAY LIBRARIES; PHAGE DISPLAY TECHNOLOGY; PHAGE PARTICLES; RE-INFECTION; RECOMBINANT ANTIBODY; SCFV ANTIBODY FRAGMENTS; STAPHYLOCOCCAL ENTEROTOXIN B; STAPHYLOCOCCUS AUREUS; SUPERANTIGEN;

ALLERGIES; CLONING; GENES; MERGERS AND ACQUISITIONS; MONOCLONAL ANTIBODIES; SURFACE PLASMON RESONANCE;

CHEMICAL DETECTION;

DIAGNOSTIC AGENT; ENTEROTOXIN; ENTEROTOXIN B, STAPHYLOCOCCAL; PEPTIDE LIBRARY; RECOMBINANT PROTEIN; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY; SUPERANTIGEN;

ANTIBODY; BACTERIUM; CLONE; GENETIC ANALYSIS; IMMUNOASSAY; RECOMBINATION; TOXIN;

ANIMAL; ANTIBODY AFFINITY; ARTICLE; DNA SEQUENCE; GENETICS; IMMUNOLOGY; MOLECULAR GENETICS; MOUSE; NUCLEOTIDE SEQUENCE;

ANIMALS; ANTIBODY AFFINITY; ENTEROTOXINS; MICE; MOLECULAR SEQUENCE DATA; PEPTIDE LIBRARY; RECOMBINANT PROTEINS; SEQUENCE ANALYSIS, DNA; SINGLE-CHAIN ANTIBODIES; SUPERANTIGENS;

BACTERIA (MICROORGANISMS); MURINAE; STAPHYLOCOCCUS AUREUS;

EID: 78650373185     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.01441-10     Document Type: Article

References (43)

1. Altschul, S. F., W. Gish, W. Miller, E. W. Mayers, and D. J. Lipman. 1990. Basic local alignment search tool. J. Mol. Biol. 215:403-410.
2. Azzazy, H. M. E., and W. E. Highsmith, Jr. 2002. Phage display technology: Clinical applications and recent innovations. Clin. Biochem. 35:425-445.
3. Baker, M. D., A. C. Papageorgiou, R. W. Titball, J. Miller, S. White, B. Lingard, J. J. Lee, D. Cavanagh, M. A. Kehoe, J. H. Robinson, and K. R. Acharya. 2002. Structural and functional role of threonine 112 in a superantigen Staphylococcus aureus enterotoxin B. J. Biol. Chem. 277:2756-2762.
4. Balaban, N., and A. Rasooly. 2000. Staphylococcal enterotoxins. Int. J. Food Microbiol. 61:1-10.
5. Bergdoll, M. S. 1983. Enterotoxins, p. 559-598. In C. S. F. Easmon and C. Adlam (ed.), Staphylococci and staphylococcal infections. Academic Press, New York, NY.
6. Casman, E. P., and R. W. Bennett. 1964. Production of antiserum for staphylococcal enterotoxin. Appl. Microbiol. 12:363-367.
7. Chothia, C., and A. M. Lesk. 1987. Canonical structures for the hypervariable loops of immunoglobulins. J. Mol. Biol. 196:901-917.
8. Coia, G., P. J. Hudson, and R. A. Irving. 2001. Protein affinity maturation in vivo using E. coli mutator cells. J. Immunol. Methods 251:187-193.
9. Emanuel, P. A., J. Dang, J. S. Gebhardt, J. Aldrich, E. A. Garber, H. Kulaga, P. Stopa, J. J. Valdes, and A. Dion-Schultz. 2000. Recombinant antibodies: A new reagent for biological agent detection. Biosens. Bioelectron. 14:751-759.
10. Emanuel, P., T. O'Brien, J. Burans, B. R. DasGupta, J. J. Valdes, and M. Eldefrawi. 1996. Directing antigen specificity towards botulinum neurotoxin with combinatorial phage display libraries. J. Immunol. Methods 193:189-197.
11. Frame, K. K., and W. S. Hu. 1990. The loss of antibody productivity in continuous culture of hybridoma cells. Biotechnol. Bioeng. 35:469-476.
12. Giudicelli, V., D. Chaume, and M. P. Lefranc. 2004. IMGT/V-QUEST, an integrated software program for immunoglobulin and T cell receptor V-J and V-D-J rearrangement analysis. Nucleic Acids Res. 32:435-440.
13. Harlow, E., and D. Lane. 1988. Antibodies: A laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
14. Hayhurst, A., S. Happe, R. Mabry, Z. Koch, B. L. Iverson, and G. Georgiou. 2003. Isolation and expression of recombinant antibody fragments to the biological warfare pathogen Brucella melitensis. J. Immunol. Methods 276: 185-196.
15. Heng, C. K., T. C. Seng, N. Khalid, J. A. Harikrishna, and R. Y. Othman. 2003. Synthesis of a soluble flag-tagged single chain variable fragment (scFv) antibody targeting cucumber mosaic virus (CMV) coat protein. Asia Pac. J. Mol. Biol. Biotechnol. 11:93-100.
16. Hoogenboom, H. R., A. D. Griffiths, K. S. Johnson, D. J. Chriswell, P. Hudson, and G. Winter. 1991. Multi-subunit proteins on the surface of filamentous phage: Methodologies for displaying antibody (Fab) heavy and light chains. Nucleic Acids Res. 19:4133-4137.
17. Hoogenboom, H. R., A. P. de Bruin, S. E. Hufton, R. M. Hoet, J. W. Arends, and R. C. Roovers. 1998. Antibody phage display technology and its applications. Immunotechnology 4:1-20.
18. Ikematsu, H., Y. Ichiyoshi, E. W. Schettino, M. Nakamura, and P. Casali. 1994. VH and V kappa segment structure of anti-insulin IgG autoantibodies in patients with insulin-dependent diabetes mellitus: Evidence for somatic selection. J. Immunol. 152:1430-1441.
19. Jurado, P., D. Ritz, J. Beckwith, V. de Lorenzo, and L. A. Fernandez. 2002. Production of functional single-chain Fv antibodies in the cytoplasm of Escherichia coli. J. Mol. Biol. 320:1-10.
20. Kabat, E. A., T. T. Wu, H. M. Perry, K. S. Gottesman, and C. Foeller. 1991. Sequences of proteins of immunological interest, 5th ed. National Institutes of Health, U.S. Department of Health and Human Services, Bethesda, MD.
21. Kamboj, D. V., A. K. Goel, and L. Singh. 2006. Biological warfare agents. Def. Sci. J. 56:495-506.
22. Kamboj, D. V., V. Nema, A. K. Pandey, A. K. Goel, and L. Singh. 2006. Heterologous expression of staphylococcal enterotoxin B (seb) gene for antibody production. Electron. J. Biotechnol. www.ejbiotechnology.info /content/vol9/issue5/full/3/index.html.
23. Kessler, N., M. Aymard, and S. Bertrand. 1993. Stability of a murine hybridoma is dependent on the clone line and culture media. In Vitro Cell. Dev. Biol. 29A:203-207.
24. Kortt, A. A., M. Lah, G. W. Oddie, C. L. Gruen, J. E. Burns, L. A. Pearce, J. L. Atwell, A. J. McCoy, G. J. Howlett, D. W. Metzger, R. G. Webster, and P. J. Hudson. 1997. Single-chain Fv fragments of anti-neuraminidase antibody NC10 containing five- and ten-residue linkers form dimers and with zero-residue linker a trimer. Protein Eng. 10:423-433.
25. Lee, M. H., and J. W. Kwak. 2003. Expression and functional reconstitution of a recombinant antibody (Fab') specific for human apolipoprotein B-100. J. Biotechnol. 101:189-198.
26. Llewelyn, M., and J. Cohen. 2002. Superantigens: Microbial agents that corrupt immunity. Lancet Infect. Dis. 2:156-162.
27. Mantis, N. J. 2005. Vaccines against the category B toxins: Staphylococcal enterotoxin B, epsilon toxin and ricin. Adv. Drug Deliv. Rev. 57:1424-1439.
28. Mattix, M. E., R. E. Hunt, C. L. Wilhelmson, A. J. Johnson, and W. B. Baze. 1995. Aerosolized staphylococcal enterotoxin B-induced pulmonary lesions in rhesus monkeys (Macaca mulatta). Toxicol. Pathol. 23:262-268.
29. Meyer, T., J. Stratmann-Selke, J. Meens, T. Schirmann, G. F. Gerlach, R. Frank, S. Dübel, K. Strutzberg-Minder, and M. Hust. 2010. Isolation of scFv fragment specific to OmpD of Salmonella typhimurium. Vet. Microbiol., in press. doi:10.1016/j.vetmic.2010.06.023.
30. McCormick, J. K., J. M. Yarwood, and P. M. Schlievert. 2001. Toxic shock syndrome and bacterial superantigens: An update. Annu. Rev. Microbiol. 55:77-104.
31. Mechaly, A., E. Zahavy, and M. Fisher. 2008. Development and implementation of a single-chain Fv antibody for specific detection of Bacillus anthracis spores. Appl. Environ. Microbiol. 74:818-822.
32. Nema, V., R. Agrawal, D. V. Kamboj, A. K. Goel, and L. Singh. 2007. Isolation and characterization of heat resistant enterotoxigenic Staphylococcus aureus from a food poisoning outbreak in Indian subcontinent. Int. J. Food Microbiol. 117:29-35.
33. Ono, H. K., K. Omoe, K. Imanishi, Y. Iwakabe, D. L. Hu, H. Kato, N. Saito, A. Nakane, T. Uchiyama, and K. Shinagawa. 2008. Identification and characterization of two novel staphylococcal enterotoxins, types S and T. Infect. Immun. 76:4999-5005.
34. Paoli, G. C., L. G. Kleina, and J. D. Brewster. 2007. Development of Listeria monocytogenes-specific immunomagnetic beads using a single-chain antibody fragment. Foodborne Pathog. Dis. 4:74-83.
35. Papageorgiou, A. C., H. S. Tranter, and K. R. Acharya. 1998. Crystal structure of microbial superantigen staphylococcal enterotoxin B at 1.5 Å resolution: Implications for superantigen recognition by MHC class II molecules and T-cell receptors. J. Mol. Biol. 277:61-79.
36. Pelat, T., M. Hust, M. Hale, M. P. Lefranc, S. Dübel, and P. Thullier. 2009. Isolation of a human-like antibody fragment (scFv) that neutralizes ricin biological activity. BMC Biotechnol. 9:60. http://www.biomedcentral.com /1472-6750/9/60.
37. Rusnak, J. M., M. Kortepeter, R. Ulrich, M. Poli, and E. Boudreau. 2004. Laboratory exposure to staphylococcal enterotoxin B. Emerg. Infect. Dis. 10:1544-1549.
38. Sambrook, J., and D. W. Russel. Molecular cloning: A laboratory manual, 3rd ed. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
39. Schlievert, P. M. 1993. Role of superantigens in human disease. J. Infect. Dis. 167:997-1002.
40. Thomas, D. Y., S. Jarraud, B. Lemercier, G. Cozon, K. Echasserieau, J. Etienne, M. L. Gougeon, G. Lina, and F. Vandenesch. 2006. Staphylococcal enterotoxins like toxins U2 and V, two new staphylococcal superantigens arising from recombination within the enterotoxin gene cluster. Infect. Immun. 74:4724-4734.
41. Turner, D. J., M. A. Ritter, and A. J. George. 1997. Importance of the linker in expression of single-chain Fv antibody fragments: Optimization of peptide sequence using phage display technology. J. Immunol. Methods 205:43-54.
42. Ulrich, R. B., S. Sidell, T. J. Taylor, C. L. Wilhelmsen, and D. R. Franz. 1997. Staphylococcal enterotoxin B and related pyrogenic toxins, p. 621-630. In F. R. Sidell, E. T. Takafuji, and D. R. Franz (ed.), Textbook of military medicine, warfare, weaponry, and the casualty. Medical aspects of chemical and biological warfare. Office of the Surgeon General, Department of the Army, Falls Church, VA.
43. Wang, S. H., J. B. Zhang, Z. P. Zhang, Y. F. Zhou, R. F. Yang, J. Chen, Y. C. Guo, F. You, and X. E. Zhang. 2006. Construction of single chain variable fragment (scFv) and biscFv-alkaline phosphatase fusion protein for detection of Bacillus anthracis. Anal. Chem. 78:997-1004.