Construction of single chain variable fragment (ScFv) and BiscFv-alkaline phosphatase fusion protein for detection of Bacillus Anthracis

Analytical Chemistry

Volumn 78, Issue 4, 2006, Pages 997-1004

  Wang, Shi Hua ^a,c   Zhang, Ji Bin ^a,b   Zhang, Zhi Ping ^a   Zhou, Ya Feng ^a   Yang, Rui Fu ^d   Chen, Jia ^a   Guo, Yong Chao ^a   You, Fan ^a   Zhang, Xian En ^a  

^a WUHAN INSTITUTE OF VIROLOGY   (China)

^b HUAZHONG AGRICULTURAL UNIVERSITY   (China)

^c FUJIAN AGRICULTURE AND FORESTRY UNIVERSITY   (China)

^d BEIJING INSTITUTE OF MICROBIOLOGY AND EPIDEMIOLOGY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

BACILLUS ANTHRACIS; BACTERIAL CELLS; BISCFV-ALKALINE PHOSPHATASE; SINGLE CHAIN VARIABLE FRAGMENT;

ANTIGENS; BACTERIA; CELL CULTURE; DNA SEQUENCES; ESCHERICHIA COLI; GENETIC ENGINEERING;

PROTEINS;

ALKALINE PHOSPHATASE; BACTERIAL ANTIGEN; HYBRID PROTEIN; MESSENGER RNA; RECOMBINANT ANTIBODY; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY;

ANIMAL CELL; ANIMAL EXPERIMENT; ANTIGEN BINDING; ANTIGEN SPECIFICITY; ARTICLE; BACILLUS ANTHRACIS; BACTERIAL CELL; BACTERIUM DETECTION; BINDING AFFINITY; CLONE; CONTROLLED STUDY; DNA SEQUENCE; ENZYME ACTIVITY; ENZYME LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; FEMALE; GENE AMPLIFICATION; GENE INSERTION; GENE VECTOR; IMMUNIZATION; MOUSE; NONHUMAN; PHAGE DISPLAY; PHAGEMID; PROTEIN EXPRESSION; PROTEIN MICROARRAY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY;

ALKALINE PHOSPHATASE; AMINO ACID SEQUENCE; ANIMALS; BACILLUS ANTHRACIS; BASE SEQUENCE; BLOTTING, WESTERN; DNA, RECOMBINANT; ELECTROPHORESIS, POLYACRYLAMIDE GEL; IMMUNOGLOBULIN FRAGMENTS; MICE; MOLECULAR SEQUENCE DATA; RECOMBINANT FUSION PROTEINS;

EID: 33144455961     PISSN: 00032700     EISSN: None     Source Type: Journal    
DOI: 10.1021/ac0512352     Document Type: Article

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