Construction and diversification of yeast cell surface displayed libraries by yeast mating: Application to the affinity maturation of Fab antibody fragments

Gene

Volumn 342, Issue 2, 2004, Pages 211-218

  Blaise, Lydia ^a   Wehnert, Anita ^a   Steukers, Mieke P G ^a   Van Den Beucken, Twan ^a   Hoogenboom, Hennie R ^a   Hufton, Simon E ^a  

^a Building 22   (Belgium)

Author keywords

Antibody mutagenesis; Chain shuffling; Combinatorial Fab library; Molecular evolution; Selection; Yeast display

Indexed keywords

IMMUNOGLOBULIN F(AB) FRAGMENT; IMMUNOGLOBULIN HEAVY CHAIN; IMMUNOGLOBULIN LIGHT CHAIN;

AMINO ACID SEQUENCE; ANALYTICAL ERROR; ANTIBODY AFFINITY; ANTIBODY LIBRARY; ARTICLE; CELL SURFACE; COMBINATORIAL LIBRARY; CONTROLLED STUDY; FLOW CYTOMETRY; FLUORESCENCE ACTIVATED CELL SORTING; FUNGAL STRAIN; GENETIC VARIABILITY; HAPLOIDY; MATING; MOLECULAR CLONING; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SACCHAROMYCES CEREVISIAE; YEAST CELL;

ANTIBODY AFFINITY; CELL WALL; CLONING, MOLECULAR; DIPLOIDY; FLOW CYTOMETRY; GENETIC VECTORS; HAPLOIDY; HUMANS; IMMUNOGLOBULIN FAB FRAGMENTS; MUTATION; PEPTIDE LIBRARY; SACCHAROMYCES CEREVISIAE; STREPTAVIDIN;

SACCHAROMYCES; SACCHAROMYCES CEREVISIAE;

EID: 7544241938     PISSN: 03781119     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.gene.2004.08.014     Document Type: Article

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