Germline transgenesis in pigs by cytoplasmic microinjection of Sleeping Beauty transposons

Nature Protocols

Volumn 9, Issue 4, 2014, Pages 810-827

  Ivics, Zoltán ^a   Garrels, Wiebke ^b   Mátés, Lajos ^c   Yau, Tien Yin ^d   Bashir, Sanum ^e   Zidek, Vaclav ^f   Landa, Vladimír ^f   Geurts, Aron ^g   Pravenec, Michal ^f   Rülicke, Thomas ^d   Kues, Wilfried A ^b   Izsvák, Zsuzsanna ^e  

^a PAUL EHRLICH INSTITUT   (Germany)

^b FEDERAL RESEARCH INSTITUTE FOR ANIMAL HEALTH   (Germany)

^c INSTITUTE OF EXPERIMENTAL MEDICINE   (Hungary)

^d UNIVERSITY OF VETERINARY MEDICINE   (Austria)

^e MAX DELBRÜCK CENTER FOR MOLECULAR MEDICINE   (Germany)

^f INSTITUTE OF PHYSIOLOGY   (Czech Republic)

^g MEDICAL COLLEGE OF WISCONSIN   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

GENOMIC DNA; LENTIVIRUS VECTOR; PLASMID VECTOR; SB100X TRANSPOSASE; TRANSPOSASE; UNCLASSIFIED DRUG; TRANSPOSON;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; BINDING SITE; CELL NUCLEUS TRANSPLANTATION; CLINICAL PROTOCOL; CROSS BREEDING; CYTOPLASMIC MICROINJECTION; EMBRYO; EMBRYO TRANSFER; EXPERIMENTAL DESIGN; GENE CASSETTE; GENE EXPRESSION; GENE INSERTION; GENOME; GENOTYPE; GERMLINE TRANSGENESIS; INTERMETHOD COMPARISON; MICROINJECTION; MOLECULAR CLONING; NONHUMAN; PIGLET; PRIORITY JOURNAL; PRONUCLEAR MICROINJECTION; SLEEPING BEAUTY TRANSPOSON; SOMATIC CELL NUCLEAR TRANSFER; SWINE; TRANSGENE; TRANSGENIC ANIMAL; TRANSGENICS; TRANSPOSON; ZYGOTE; ANIMAL; FEMALE; GENE TRANSFER; GENE VECTOR; GENETICS; GERM CELL; MALE; PIG; ANIMAL EXPERIMENT; BIOSAFETY; CONTROLLED STUDY; CYTOPLASM; GENE SILENCING; PLASMID; SOMATIC CELL;

ANIMALS; ANIMALS, GENETICALLY MODIFIED; DNA TRANSPOSABLE ELEMENTS; FEMALE; GENE TRANSFER TECHNIQUES; GENETIC VECTORS; GENOME; GERM CELLS; MALE; MICROINJECTIONS; SWINE; TRANSPOSASES;

EID: 84897143787     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2014.010     Document Type: Article

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