Brief report: Chimeric pigs produced from induced pluripotent stem cells demonstrate germline transmission and no evidence of tumor formation in young pigs

Stem Cells

Volumn 29, Issue 10, 2011, Pages 1640-1643

  West, Franklin D ^a   Uhl, Elizabeth W ^b   Liu, Yubing ^a,c   Stowe, Heather ^d   Lu, Yangqing ^a,c   Yu, Ping ^a   Gallegos Cardenas, Amalia ^a   Pratt, Scott L ^d   Stice, Steven L ^a,b  

^a UNIVERSITY OF GEORGIA   (United States)

^b UNIVERSITY OF GEORGIA   (United States)

^c GUANGXI UNIVERSITY   (China)

^d CLEMSON UNIVERSITY   (United States)

Author keywords

Cellular therapy; Chimeric; Germline; iPS; Pig model

Indexed keywords

OCTAMER TRANSCRIPTION FACTOR 4; TRANSCRIPTION FACTOR NANOG;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; CARCINOGENESIS; CARCINOGENICITY; EXPERIMENTAL PIG; FEMALE; GENE EXPRESSION; GENETIC MANIPULATION; LIVE BIRTH; MALE; NONHUMAN; ONCOGENE C MYC; PIGLET; PLURIPOTENT STEM CELL; POLYMERASE CHAIN REACTION; STEM CELL TRANSPLANTATION;

ANIMALS; ANIMALS, GENETICALLY MODIFIED; CELL TRANSFORMATION, NEOPLASTIC; CHIMERA; FEMALE; GENE EXPRESSION REGULATION, NEOPLASTIC; GERM CELLS; HOMEODOMAIN PROTEINS; HUMANS; INDUCED PLURIPOTENT STEM CELLS; MALE; OCTAMER TRANSCRIPTION FACTOR-3; PROTO-ONCOGENE PROTEINS C-MYC; SEQUENCE ANALYSIS, DNA; SWINE;

ANIMALIA; IPS; RODENTIA; SUIDAE; SUS;

EID: 80053221986     PISSN: 10665099     EISSN: None     Source Type: Journal    
DOI: 10.1002/stem.713     Document Type: Article

References (29)

1. Esteban MA, Xu J, Yang J et al. Generation of induced pluripotent stem cell lines from Tibetan miniature pig. J Biol Chem 2009;284:17634-17640.
2. Ezashi T, Telugu BP, Alexenko AP et al. Derivation of induced pluripotent stem cells from pig somatic cells. Proc Natl Acad Sci USA 2009;106:10993-10998.
3. West FD, Terlouw SL, Kwon DJ et al. Porcine induced pluripotent stem cells produce chimeric offspring. Stem Cells Dev 2010;19:1211-1220.
4. Wu Z, Chen J, Ren J et al. Generation of pig-induced pluripotent stem cells with a drug-inducible system. J Mol Cell Biol 2009;1:46-54.
5. Okita K, Ichisaka T, Yamanaka S. Generation of germline-competent induced pluripotent stem cells. Nature 2007;448:313-317. (Pubitemid 47080336)
6. Wernig M, Meissner A, Foreman R et al. In vitro reprogramming of fibroblasts into a pluripotent ES-cell-like state. Nature 2007;448:318-324. (Pubitemid 47080337)
7. Okita K, Nakagawa M, Hyenjong H et al. Generation of mouse induced pluripotent stem cells without viral vectors. Science 2008;322:949-953.
8. Miura K, Okada Y, Aoi T et al. Variation in the safety of induced pluripotent stem cell lines. Nat Biotechnol 2009;27:743-745.
9. Nakagawa M, Koyanagi M, Tanabe K et al. Generation of induced pluripotent stem cells without Myc from mouse and human fibroblasts. Nat Biotechnol 2008;26:101-106.
10. Gragerov A, Horie K, Pavlova M et al. Large-scale, saturating insertional mutagenesis of the mouse genome. Proc Natl Acad Sci USA 2007;104:14406-14411. (Pubitemid 350003274)
11. Pettitt SJ, Liang Q, Rairdan XY et al. Agouti C57BL/6N embryonic stem cells for mouse genetic resources. Nat Methods 2009;6:493-495.
12. Strojek RM, Reed MA, Hoover JL et al. A method for cultivating morphologically undifferentiated embryonic stem cells from porcine blastocysts. Theriogenology 1990;33:901-913.
13. Li M, Zhang D, Hou Y et al. Isolation and culture of embryonic stem cells from porcine blastocysts. Mol Reprod Dev 2003;65:429-434. (Pubitemid 36841893)
14. Piedrahita JA, Anderson GB, Bondurant RH. On the isolation of embryonic stem cells: Comparative behavior of murine, porcine and ovine embryos. Theriogenology 1990;34:879-901.
15. Blomberg LA, Schreier LL, Talbot NC. Expression analysis of pluripotency factors in the undifferentiated porcine inner cell mass and epiblast during in vitro culture. Mol Reprod Dev 2008;75:450-463. (Pubitemid 351214146)
16. Mitalipova M, Beyhan Z, First NL. Pluripotency of bovine embryonic cell line derived from precompacting embryos. Cloning 2001;3:59-67. (Pubitemid 32735029)
17. Li X, Zhou SG, Imreh MP et al. Horse embryonic stem cell lines from the proliferation of inner cell mass cells. Stem Cells Dev 2006;15:523-531. (Pubitemid 44701172)
18. Rapp UR, Korn C, Ceteci F et al. MYC is a metastasis gene for non-small-cell lung cancer. PLoS ONE 2009;4:e6029.
19. Duensing S, Darr S, Cuevas R et al. Tripeptidyl peptidase II is required for c-MYC-induced centriole overduplication and a novel therapeutic target in c-MYC-associated neoplasms. Genes Cancer 2010;1:883-892.
20. Jain M, Arvanitis C, Chu K et al. Sustained loss of a neoplastic phenotype by brief inactivation of MYC. Science 2002;297:102-104. (Pubitemid 34743102)
21. Bieche I, Laurendeau I, Tozlu S et al. Quantitation of MYC gene expression in sporadic breast tumors with a real-time reverse transcription-PCR assay. Cancer Res 1999;59:2759-2765. (Pubitemid 29283103)
22. Berns K, Hijmans EM, Bernards R. Repression of c-Myc responsive genes in cycling cells causes G1 arrest through reduction of cyclin E/CDK2 kinase activity. Oncogene 1997;15:1347-1356. (Pubitemid 27429751)
23. Palmer SJ, Burgoyne PS. In situ analysis of fetal, prepuberal and adult XX - XY chimaeric mouse testes: Sertoli cells are predominantly, but not exclusively, Xy. Development 1991;112:265-268.
24. Burgoyne PS, Buehr M, Koopman P et al. Cell-autonomous action of the testis-determining gene: Sertoli cells are exclusively XY in XX - XY chimaeric mouse testes. Development 1988;102:443-450. (Pubitemid 18086028)
25. Mazeyrat S, Saut N, Grigoriev V et al. A Y-encoded subunit of the translation initiation factor Eif2 is essential for mouse spermatogenesis. Nat Genet 2001;29:49-53. (Pubitemid 32801807)
26. Toure A, Szot M, Mahadevaiah SK et al. A new deletion of the mouse Y chromosome long arm associated with the loss of Ssty expression, abnormal sperm development and sterility. Genetics 2004;166:901-912. (Pubitemid 38396776)
27. Sun C, Skaletsky H, Birren B et al. An azoospermic man with a de novo point mutation in the Y-chromosomal gene USP9Y. Nat Genet 1999;23:429-432.
28. Foresta C, Ferlin A, Moro E. Deletion and expression analysis of AZFa genes on the human Y chromosome revealed a major role for DBY in male infertility. Hum Mol Genet 2000;9:1161-1169. (Pubitemid 30248601)
29. Burgoyne PS, Mahadevaiah SK, Sutcliffe MJ et al. Fertility in mice requires X-Y pairing and a Y-chromosomal "spermiogenesis" gene mapping to the long arm. Cell 1992;71:391-398.