Germline transgenesis in rabbits by pronuclear microinjection of Sleeping Beauty transposons

Nature Protocols

Volumn 9, Issue 4, 2014, Pages 794-809

  Ivics, Zoltán ^a   Hiripi, László ^b   Hoffmann, Orsolya I ^b   Mátés, Lajos ^c   Yau, Tien Yin ^d   Bashir, Sanum ^e   Zidek, Vaclav ^f   Landa, Vladimír ^f   Geurts, Aron ^g   Pravenec, Michal ^f   Rülicke, Thomas ^d   Bösze, Zsuzsanna ^b   Izsvák, Zsuzsanna ^e  

^a PAUL EHRLICH INSTITUT   (Germany)

^b SZENT ISTVÁN UNIVERSITY   (Hungary)

^c INSTITUTE OF EXPERIMENTAL MEDICINE   (Hungary)

^d UNIVERSITY OF VETERINARY MEDICINE   (Austria)

^e MAX DELBRÜCK CENTER FOR MOLECULAR MEDICINE   (Germany)

^f INSTITUTE OF PHYSIOLOGY   (Czech Republic)

^g MEDICAL COLLEGE OF WISCONSIN   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

GENOMIC DNA; MESSENGER RNA; SYNTHETIC MRNA; TRANSPOSASE MRNA; UNCLASSIFIED DRUG; PLASMID DNA; TRANSPOSASE; TRANSPOSON;

ANIMAL EXPERIMENT; ARTICLE; BINDING SITE; CLINICAL PROTOCOL; CONTROLLED STUDY; EMBRYO; GENE CASSETTE; GENE EXPRESSION SYSTEM; GENE INSERTION; GENE SILENCING; GERM LINE; MICROINJECTION; NONHUMAN; OOCYTE; PLASMID; PRIORITY JOURNAL; RABBIT; RNA TRANSLATION; SLEEPING BEAUTY TRANSPOSON SYSTEM; TRANSGENICS; TRANSPOSON; GENE EXPRESSION; GERMLINE TRANSGENESIS; PRONUCLEAR MICROINJECTION; PRONUCLEUS; SLEEPING BEAUTY TRANSPOSON; TRANSGENE; TRANSGENIC ANIMAL; ANIMAL; FEMALE; GENE TRANSFER; GENETICS; GERM CELL; MALE; TIME;

ANIMALS; ANIMALS, GENETICALLY MODIFIED; DNA TRANSPOSABLE ELEMENTS; FEMALE; GENE TRANSFER TECHNIQUES; GERM CELLS; MALE; MICROINJECTIONS; RABBITS; TIME FACTORS; TRANSPOSASES;

EID: 84897143633     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2014.009     Document Type: Article

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