Distinct BimBH3 (BimSAHB) stapled peptides for structural and cellular studies

ACS Chemical Biology

Volumn 9, Issue 3, 2014, Pages 831-837

  Bird, Greg H ^a   Gavathiotis, Evripidis ^b   Labelle, James L ^c   Katz, Samuel G ^d   Walensky, Loren D ^a  

^a DANA FARBER CANCER INSTITUTE   (United States)

^b ALBERT EINSTEIN COLLEGE OF MEDICINE   (United States)

^c UNIVERSITY OF CHICAGO   (United States)

^d YALE UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BIM PROTEIN; HYDROCARBON; HYDROCARBON STAPLED BIM BH3 PEPTIDE; UNCLASSIFIED DRUG; ONCOPROTEIN; PEPTIDE FRAGMENT;

AMINO ACID SEQUENCE; ARTICLE; BINDING AFFINITY; BINDING SITE; BIOLOGY; CELL ACTIVITY; CHEMISTRY; MEDICAL RESEARCH; NONHUMAN; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; PRIORITY JOURNAL; PROTEIN INTERACTION; PROTEIN STRUCTURE; WILD TYPE; ANIMAL; APOPTOSIS; HUMAN; METABOLISM;

ANIMALS; APOPTOSIS; HUMANS; PEPTIDE FRAGMENTS; PROTO-ONCOGENE PROTEINS;

EID: 84896923199     PISSN: 15548929     EISSN: 15548937     Source Type: Journal    
DOI: 10.1021/cb4003305     Document Type: Article

References (22)

1. Schafmeister, C., Po, J., and Verdine, G. (2000) An all-hydrocarbon cross-linking system for enhancing the helicity and metabolic stability of peptides J. Am. Chem. Soc. 122, 5891-5892
2. Bird, G. H., Bernal, F., Pitter, K., and Walensky, L. D. (2008) Synthesis and biophysical characterization of stabilized alpha-helices of BCL-2 domains Methods Enzymol. 446, 369-386
3. Bird, G. H., Crannell, C. W., and Walensky, L. D. (2011) Chemical synthesis of hydrocarbon-stapled peptides for protein interaction research and therapeutic targeting Curr. Protoc. Chem. Biol. 3, 99-117
4. Okamoto, T., Zobel, K., Fedorova, A., Quan, C., Yang, H., Fairbrother, W. J., Huang, D. C., Smith, B. J., Deshayes, K., and Czabotar, P. E. (2013) Stabilizing the pro-apoptotic BimBH3 helix (BimSAHB) does not necessarily enhance affinity or biological activity ACS Chem. Biol. 8, 297-302
5. Gavathiotis, E., Suzuki, M., Davis, M. L., Pitter, K., Bird, G. H., Katz, S. G., Tu, H. C., Kim, H., Cheng, E. H., Tjandra, N., and Walensky, L. D. (2008) BAX activation is initiated at a novel interaction site Nature 455, 1076-1081
6. LaBelle, J. L., Katz, S. G., Bird, G. H., Gavathiotis, E., Stewart, M. L., Lawrence, C., Fisher, J. K., Godes, M., Pitter, K., Kung, A. L., and Walensky, L. D. (2012) A stapled BIM peptide overcomes apoptotic resistance in hematologic cancers J. Clin. Invest. 122, 2018-2031
7. Bernal, F., Tyler, A. F., Korsmeyer, S. J., Walensky, L. D., and Verdine, G. L. (2007) Reactivation of the p53 tumor suppressor pathway by a stapled p53 peptide J. Am. Chem. Soc. 129, 2456-2457
8. Stewart, M. L., Fire, E., Keating, A. E., and Walensky, L. D. (2010) The MCL-1 BH3 helix is an exclusive MCL-1 inhibitor and apoptosis sensitizer Nat. Chem. Biol. 6, 595-601
9. Walensky, L. D., Kung, A. L., Escher, I., Malia, T. J., Barbuto, S., Wright, R. D., Wagner, G., Verdine, G. L., and Korsmeyer, S. J. (2004) Activation of apoptosis in vivo by a hydrocarbon-stapled BH3 helix Science 305, 1466-1470
10. Walensky, L. D., Pitter, K., Morash, J., Oh, K. J., Barbuto, S., Fisher, J., Smith, E., Verdine, G. L., and Korsmeyer, S. J. (2006) A stapled BID BH3 helix directly binds and activates BAX Mol. Cell 24, 199-210
11. Suzuki, M., Youle, R. J., and Tjandra, N. (2000) Structure of Bax: coregulation of dimer formation and intracellular localization Cell 103, 645-654
12. Gavathiotis, E., Reyna, D. E., Bellairs, J. A., Leshchiner, E. S., and Walensky, L. D. (2012) Direct and selective small-molecule activation of proapoptotic BAX Nat. Chem. Biol. 8, 639-645
13. Gavathiotis, E., Reyna, D. E., Davis, M. L., Bird, G. H., and Walensky, L. D. (2010) BH3-triggered structural reorganization drives the activation of proapoptotic BAX Mol. Cell 40, 481-492
14. Walensky, L. D. and Gavathiotis, E. (2011) BAX unleashed: the biochemical transformation of an inactive cytosolic monomer into a toxic mitochondrial pore Trends Biochem. Sci. 36, 642-652
15. Adams, J. M. (2012) Therapeutic potential of a peptide targeting BCL-2 cell guardians in cancer J. Clin. Invest. 122, 1965-1967
16. Willis, S. N., Fletcher, J. I., Kaufmann, T., van Delft, M. F., Chen, L., Czabotar, P. E., Ierino, H., Lee, E. F., Fairlie, W. D., Bouillet, P., Strasser, A., Kluck, R. M., Adams, J. M., and Huang, D. C. (2007) Apoptosis initiated when BH3 ligands engage multiple Bcl-2 homologs, not Bax or Bak Science 315, 856-859
17. Czabotar, P. E., Westphal, D., Dewson, G., Ma, S., Hockings, C., Fairlie, W. D., Lee, E. F., Yao, S., Robin, A. Y., Smith, B. J., Huang, D. C., Kluck, R. M., Adams, J. M., and Colman, P. M. (2013) Bax crystal structures reveal how BH3 domains activate Bax and nucleate its oligomerization to induce apoptosis Cell 152, 519-531
18. Braun, C. R., Mintseris, J., Gavathiotis, E., Bird, G. H., Gygi, S. P., and Walensky, L. D. (2010) Photoreactive stapled BH3 peptides to dissect the BCL-2 family interactome Chem. Biol. 17, 1325-1333
19. Edwards, A. L., Gavathiotis, E., LaBelle, J. L., Braun, C. R., Opoku-Nsiah, K. A., Bird, G. H., and Walensky, L. D. (2013) Multimodal interaction with BCL-2 family proteins underlies the proapoptotic activity of PUMA BH3 Chem. Biol. 20, 888-902
20. Leshchiner, E. S., Braun, C. R., Bird, G. H., and Walensky, L. D. (2013) Direct activation of full-length proapoptotic BAK Proc. Natl. Acad. Sci. U.S.A. 10.1073/pnas.1214313110
21. Kim, H., Tu, H. C., Ren, D., Takeuchi, O., Jeffers, J. R., Zambetti, G. P., Hsieh, J. J., and Cheng, E. H. (2009) Stepwise activation of BAX and BAK by tBID, BIM, and PUMA initiates mitochondrial apoptosis Mol. Cell 36, 487-499
22. Pitter, K., Bernal, F., Labelle, J., and Walensky, L. D. (2008) Dissection of the BCL-2 family signaling network with stabilized alpha-helices of BCL-2 domains Methods Enzymol. 446, 387-408