Activation of apoptosis in vivo by a hydrocarbon-stapled BH3 helix

Science

Volumn 305, Issue 5689, 2004, Pages 1466-1470

  Walensky, Loren D ^a,b   Kung, Andrew L ^b,c   Escher, Iris ^d   Malia, Thomas J ^e,f   Barbuto, Scott ^a   Wright, Renee D ^c   Wagner, Gerhard ^e   Verdine, Gregory L ^d   Korsmeyer, Stanley J ^a  

^a HOWARD HUGHES MEDICAL INSTITUTE   (United States)

^b BOSTON CHILDREN S HOSPITAL   (United States)

^c DANA FARBER CANCER INSTITUTE   (United States)

^d HARVARD UNIVERSITY   (United States)

^e HARVARD MEDICAL SCHOOL   (United States)

^f MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

APOPTOSIS; HYDROCARBON STAPLING;

CELLS; HYDROCARBONS; MODULATION;

PROTEINS;

CYTOCHROME C; FLUORESCEIN ISOTHIOCYANATE; HYDROCARBON; PROTEIN BAK; PROTEIN BAX; PROTEIN BCL 2; PROTEIN BCL X;

BIOLOGY; PROTEIN;

ANIMAL TISSUE; APOPTOSIS; ARTICLE; CIRCULAR DICHROISM; CONFOCAL MICROSCOPY; ENZYME LINKED IMMUNOSORBENT ASSAY; FLUORESCENCE POLARIZATION; HUMAN; HUMAN CELL; IN VIVO STUDY; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN PROTEIN INTERACTION; SEQUENCE HOMOLOGY;

ALKENES; ANIMALS; APOPTOSIS; BH3 INTERACTING DOMAIN DEATH AGONIST PROTEIN; BRIDGED COMPOUNDS; CARRIER PROTEINS; CELL DIVISION; CELL LINE, TUMOR; CELL MEMBRANE; CYTOCHROMES C; DOSE-RESPONSE RELATIONSHIP, DRUG; ENDOSOMES; HUMANS; JURKAT CELLS; LEUKEMIA, EXPERIMENTAL; LEUKEMIC INFILTRATION; MICE; MICE, SCID; MITOCHONDRIA, LIVER; MOLECULAR MIMICRY; NEOPLASM TRANSPLANTATION; PEPTIDE FRAGMENTS; PEPTIDES; PROTEIN BINDING; PROTEIN ENGINEERING; PROTEIN STRUCTURE, SECONDARY; PROTEIN STRUCTURE, TERTIARY; PROTO-ONCOGENE PROTEINS; PROTO-ONCOGENE PROTEINS C-BCL-2; TRANSPLANTATION, HETEROLOGOUS;

ANIMALIA;

EID: 4444291734     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.1099191     Document Type: Article

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33. We thank D. Brown and M. Salanga for assistance with confocal microscopy; D. Neuberg and M. Goldwasser for biostatistics support; E. Gillespie and W. Beavers for assistance with automated peptide synthesis and amino acid analysis; S. Armstrong for providing leukemia cell lines; R. Bronson for rodent necropsy evaluation; Q. Liao for assistance with mass spectrometry; S. Lux, B. Malynn, and F. Bernal for helpful discussions; and E. Smith for editorial and computer graphics assistance. L.D.W. is a Lymphoma Research Foundation Fellow and is also supported by National Heart, Lung, and Blood Institute grant no. K08HL074049, an American Society of Hematology Scholar Award, and the Lauri Strauss Leukemia Foundation. This work is supported in part by NIH grant no. R37CA50239, a Leukemia and Lymphoma Society deVilliers International Achievement Award to S.J.K., a grant from the Virginia and D.K. Ludwig Fund for Cancer Research to G.W., and a gift from Enanta Pharmaceuticals to G.L.V. Molecular interaction data have been deposited in the Biomolecular Interaction Network Database with accession codes 151034 and 151035.