A robust high throughput platform to generate functional recombinant monoclonal antibodies using rabbit B cells from peripheral blood

PLoS ONE

Volumn 9, Issue 2, 2014, Pages

  Seeber, Stefan ^a   Ros, Francesca ^a   Thorey, Irmgard ^a   Tiefenthaler, Georg ^a   Kaluza, Klaus ^a   Lifke, Valeria ^a   André, Jens ^a   Fischer, Alexander ^a   Klostermann, Stefan ^a   Endl, Josef ^a   Kopetzki, Erhard ^a   Pashine, Achal ^a,b   Siewe, Basile ^a,c   Kaluza, Brigitte ^a   Platzer, Josef ^a   Offner, Sonja ^a  

^a ROCHE DIAGNOSTICS GMBH   (Germany)

^b BRISTOL MYERS SQUIBB   (United States)

^c RUSH UNIVERSITY MEDICAL CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

IMMUNOGLOBULIN G ANTIBODY; MONOCLONAL ANTIBODY; RECOMBINANT ANTIBODY; CELL SURFACE RECEPTOR; EPITOPE; IL1RL1 PROTEIN, HUMAN; IL33 PROTEIN, HUMAN; IMMUNOGLOBULIN HEAVY CHAIN; IMMUNOGLOBULIN LIGHT CHAIN; IMMUNOGLOBULIN VARIABLE REGION; INTERLEUKIN DERIVATIVE; PROTEIN BINDING; RECOMBINANT PROTEIN;

ANIMAL CELL; ANIMAL EXPERIMENT; ANTIBODY ISOLATION; ANTIBODY PRODUCTION; ANTIBODY STRUCTURE; ANTIGEN SPECIFICITY; ARTICLE; B LYMPHOCYTE; CELL CLONING; CELL ISOLATION; CELL SELECTION; CONTROLLED STUDY; LYMPHOCYTE CULTURE; NONHUMAN; POLYMERASE CHAIN REACTION; PROCESS DEVELOPMENT; RABBIT; SEQUENCE ANALYSIS; AMINO ACID SEQUENCE; ANIMAL; ANTIBODY SPECIFICITY; BIOSYNTHESIS; BLOOD; CELL CLONE; CELL CULTURE; CELL SEPARATION; HEK293 CELL LINE; HIGH THROUGHPUT SCREENING; HUMAN; IMMUNIZATION; IMMUNOGLOBULIN VARIABLE REGION; IMMUNOLOGY; METABOLISM; PROCEDURES;

AMINO ACID SEQUENCE; ANIMALS; ANTIBODIES, MONOCLONAL; ANTIBODY SPECIFICITY; B-LYMPHOCYTES; CELL SEPARATION; CELLS, CULTURED; CLONE CELLS; EPITOPES; HEK293 CELLS; HIGH-THROUGHPUT SCREENING ASSAYS; HUMANS; IMMUNIZATION; IMMUNOGLOBULIN HEAVY CHAINS; IMMUNOGLOBULIN LIGHT CHAINS; IMMUNOGLOBULIN VARIABLE REGION; INTERLEUKINS; PROTEIN BINDING; RABBITS; RECEPTORS, CELL SURFACE; RECOMBINANT PROTEINS;

EID: 84896875573     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0086184     Document Type: Article

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