Antibody generation through B cell panning on antigen followed by in situ culture and direct RT-PCR on cells harvested en masse from antigen-positive wells

Journal of Immunological Methods

Volumn 316, Issue 1-2, 2006, Pages 133-143

  Lightwood, Daniel J ^a   Carrington, Bruce ^b   Henry, Alistair J ^b   McKnight, Andrew J ^b   Crook, Kenneth ^b   Cromie, Karen ^b   Lawson, Alastair D G ^b  

^a UCB PHARMA   (Belgium)

^b NONE

Author keywords

B cell; B cell enrichment; B cell panning; B cell selection; Hybridoma; Monoclonal antibody; Non clonal; Phage display; Plasma cell; RT PCR; SLAM

Indexed keywords

ANTIGEN; IMMUNOGLOBULIN; MONOCLONAL ANTIBODY; RECOMBINANT ANTIBODY;

ANIMAL CELL; ANTIBODY AFFINITY; ANTIBODY SPECIFICITY; ARTICLE; B LYMPHOCYTE; BINDING AFFINITY; CELL ACTIVITY; CELL CULTURE; CELL ISOLATION; CELL LINE; CELL PROLIFERATION; CONTROLLED STUDY; ENZYME LINKED IMMUNOSORBENT ASSAY; GENE EXPRESSION; IMMUNE RESPONSE; IMMUNOGLOBULIN PRODUCTION; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; RABBIT; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SCREENING; SPLEEN CELL; SUPERNATANT; THYMOMA;

AMINO ACID SEQUENCE; ANIMALS; ANTIBODIES, MONOCLONAL; B-LYMPHOCYTES; CHO CELLS; CLONE CELLS; CLONING, MOLECULAR; CRICETINAE; ENZYME-LINKED IMMUNOSORBENT ASSAY; IMMUNOGLOBULIN HEAVY CHAINS; IMMUNOGLOBULIN LIGHT CHAINS; IMMUNOGLOBULIN VARIABLE REGION; MOLECULAR SEQUENCE DATA; OX40 LIGAND; RABBITS; RECOMBINANT PROTEINS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA; SURFACE PLASMON RESONANCE;

ORYCTOLAGUS CUNICULUS;

EID: 33749983676     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jim.2006.08.010     Document Type: Article

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