A combinatorial relative mass value evaluation of endogenous bioactive proteins in three-dimensional cultured nucleus pulposus cells of herniated intervertebral discs: Identification of potential target proteins for gene therapeutic approaches

PLoS ONE

Volumn 8, Issue 11, 2013, Pages

  Mern, Demissew S ^a,b   Fontana, Johann ^b   Beierfuß, Anja ^b   Thomé, Claudius ^a   Hegewald, Aldemar A ^a,b  

^a INNSBRUCK MEDICAL UNIVERSITY   (Austria)

^b UNIVERSITY OF HEIDELBERG   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

AGGRECAN; AGGRECANASE 1; AGGRECANASE 2; BONE MORPHOGENETIC PROTEIN 2; BONE MORPHOGENETIC PROTEIN 4; BONE MORPHOGENETIC PROTEIN 6; COLLAGEN TYPE 1; COLLAGEN TYPE 2; COLLAGENASE 3; GELATINASE A; GELATINASE B; INTERLEUKIN 1 RECEPTOR; INTERLEUKIN 1BETA; INTERSTITIAL COLLAGENASE; MATRILYSIN; NEUTROPHIL COLLAGENASE; OSTEOGENIC PROTEIN 1; SOMATOMEDIN C; STROMELYSIN; STROMELYSIN 2; TISSUE INHIBITOR OF METALLOPROTEINASE 1; TISSUE INHIBITOR OF METALLOPROTEINASE 2; TISSUE INHIBITOR OF METALLOPROTEINASE 3; TISSUE INHIBITOR OF METALLOPROTEINASE 4; TRANSFORMING GROWTH FACTOR BETA1; TRANSFORMING GROWTH FACTOR BETA3; TUMOR NECROSIS FACTOR ALPHA; ADAM PROTEIN; ADAMTS5 PROTEIN, HUMAN; AGGRECANASE-1; MATRIX METALLOPROTEINASE; PROCOLLAGEN N PROTEINASE; PROTEOME; SCLEROPROTEIN; TISSUE INHIBITOR OF METALLOPROTEINASE;

ADULT; AGE; AGED; ARTICLE; CELL CULTURE; CELL PROLIFERATION; FEMALE; GENDER; HUMAN; HUMAN CELL; HUMAN TISSUE; INTERVERTEBRAL DISK DEGENERATION; LUMBAR DISK HERNIA; MAJOR CLINICAL STUDY; MALE; MIDDLE AGED; NUCLEUS PULPOSUS; PROTEIN EXPRESSION; CULTURE TECHNIQUE; CYTOLOGY; EXTRACELLULAR MATRIX; GENE EXPRESSION; GENETICS; INTERVERTEBRAL DISC DEGENERATION; INTERVERTEBRAL DISK; METABOLISM; NUCLEAR MAGNETIC RESONANCE IMAGING; VERY ELDERLY;

ADAM PROTEINS; ADULT; AGED; AGED, 80 AND OVER; CELL CULTURE TECHNIQUES; CELL PROLIFERATION; EXTRACELLULAR MATRIX; EXTRACELLULAR MATRIX PROTEINS; FEMALE; GENE EXPRESSION; HUMANS; INTERVERTEBRAL DISC; INTERVERTEBRAL DISC DEGENERATION; MAGNETIC RESONANCE IMAGING; MALE; MATRIX METALLOPROTEINASES, SECRETED; MIDDLE AGED; PROCOLLAGEN N-ENDOPEPTIDASE; PROTEOME; TISSUE INHIBITOR OF METALLOPROTEINASES;

EID: 84896697934     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0081467     Document Type: Article

References (47)

1. Hegewald AA, Ringe J, Sittinger M, Thome C (2008) Regenerative treatment strategies in spinal surgery. Front Biosci 13: 1507-1525. doi:10.2741/2777. PubMed: 17981645. (Pubitemid 351594722)
2. Mern DS, Beierfuß A, Thomé C, Hegewald AA (2012) Enhancing human nucleus pulposus cells for biological treatment approaches of degenerative intervertebral disc diseases: a systematic review. J Tissue Eng Regen Med doi: 10.1002/term 1583
3. Zhao CQ, Wang LM, Jiang LS, Dai LY (2007) The cell biology of intervertebral disc aging and degeneration. Ageing Res Rev 6: 247-261. doi:10.1016/j.arr.2007.08.001. PubMed: 17870673.
4. Kalb S, Martirosyan NL, Kalani MYS, Broc GG, Theodore N (2012) Genetics of the Degenerated Intervertebral Disc. World Neurosurg 77: 491-501. doi:10.1016/j.wneu.2011.07.014. PubMed: 22120330.
5. Nomura T, Mochida J, Okuma M, Nishimura K, Sakabe K (2001) Nucleus pulposus allograft retards intervertebral disc degeneration. Clin Orthop Relat Res 389: 94-101. doi:10.1097/00003086-200108000-00015. PubMed: 11501830. (Pubitemid 32729644)
6. Meisel HJ, Siodla V, Ganey T, Minkus Y, Hutton WC et al. (2007) Clinical experience in cell based therapeutics: disc chondrocyte transplantation a treatment for degenerated or damaged intervertebral disc. Biomol Eng 24: 5-21. doi:10.1016/j.bioeng.2006.07.002. PubMed: 16963315. (Pubitemid 46216157)
7. Sakai D, Mochida J, Yamamoto Y, Nomura T, Okuma M et al. (2003) Transplantation of mesenchymal stem cells embedded in Atelocollagen gel to the intervertebral disc: a potential therapeutic model for disc degeneration. Biomaterials 24: 3531-3541. doi:10.1016/S0142-9612(03)00222-9. PubMed: 12809782. (Pubitemid 36703127)
8. Revell PA, Damien E, Di Silvio L, Gurav N, Longinotti C et al. (2007) Tissue engineered intervertebral disc repair in the pig using injectable polymers. J Mater Sci Mater Med 18: 303-308. doi:10.1007/s10856-006-0693-6. PubMed: 17323162. (Pubitemid 46332852)
9. Nishida K, Kang JD, Gilbertson LG, Moon SH, Suh JK et al. (1999) Modulation of the biologic activity of the rabbit intervertebral disc by gene therapy: an in vivo study of adenovirusmediated transfer of the human transforming growth factor-β1 encoding gene. Spine 24: 2419-2425. doi:10.1097/00007632-199912010-00002. PubMed: 10626303. (Pubitemid 30002977)
10. Nishida K, Doita M, Takada T, Kakutani K, Miyamoto H et al. (2006) Sustained transgene expression in intervertebral disc cells in vivo mediated by microbubbleenhanced ultrasound gene therapy. Spine 31: 1415-1419. doi:10.1097/01.brs.0000219945.70675.dd. PubMed: 16741448. (Pubitemid 43880186)
11. Okuma M, Mochida J, Nishimura K, Sakabe K, Seiki K (2000) Reinsertion of stimulated nucleus pulposus cells retards intervertebral disc degeneration: an in vitro and in vivo experimental study. J Orthop Res 18: 988-997. doi:10.1002/jor.1100180620. PubMed: 11192261. (Pubitemid 32055771)
12. Sive JI, Baird P, Jeziorsk M, Watkins A, Hoyland JA et al. (2002) Expression of chondrocyte markers by cells of normal and degenerate intervertebral discs. Mol Pathol 55: 91-97. doi:10.1136/mp.55.2.91. PubMed: 11950957. (Pubitemid 34293410)
13. Millward-Sadler SJ, Costello PW, Freemont AJ, Hoyland JA (2009) Regulation of catabolic gene expression in normal and degenerate human intervertebral disc cells: implications for the pathogenesis of intervertebral disc degeneration. Arthritis Res Ther 11(3): R65. doi:10.1186/ar2693. PubMed: 19435506.
14. Le Maitre CL, Freemont AJ, Hoyland JA (2004) Localization of degradative enzymes and their inhibitors in the degenerate human intervertebral disc. J Pathol 204: 47-54. doi:10.1002/path.1608. PubMed: 15307137. (Pubitemid 39214481)
15. Bachmeier BE, Nerlich A, Mittermaier N, Weiler C, Lumenta C et al. (2009) Matrix metalloproteinase expression levels suggest distinct enzyme roles during lumbar disc herniation and degeneration. Eur Spine J 18: 1573-1586. doi:10.1007/s00586-009-1031-8. PubMed: 19466462.
16. Le Maitre CL, Hoyland JA, Freemont AJ (2007) Catabolic cytokine expression in degenerate and herniated human intervertebral discs: IL-1beta and TNF-alpha expression profile. Arthritis Res Ther 9: R77. doi:10.1186/ar2275. PubMed: 17688691.
17. Le Maitre CL, Richardson SM, Baird P, Freemont AJ, Hoyland JA (2005) Expression of receptors for putative anabolic growth factors in human intervertebral disc: implications for repair and regeneration of the disc. J Pathol 207: 445-452. doi:10.1002/path.1862. PubMed: 16278806. (Pubitemid 43025693)
18. Pfirrmann CW, Metzdorf A, Zanetti M, Hodler J, Boos N (2001) Magnetic resonance classification of lumbar intervertebral disc degeneration. Spine 26: 1873-1878. doi:10.1097/00007632-200109010-00011. PubMed: 11568697. (Pubitemid 32844681)
19. Landis RJ, Koch GG (1977) The measurement of observer agreement for categorical data. Biometrics 33: 159-174. doi:10.2307/2529310. PubMed: 843571.
20. Liebscher T, Haefeli M, Wuertz K, Nerlich AG, Boos N (2011) Age-related variation in cell density of human lumbar intervertebral discs. Spine 36: 153-159. doi:10.1097/BRS.0b013e3181cd588c. PubMed: 20671592.
21. Gruber HE, Hanley EN Jr (2002) Ultrastructure of the human intervertebral disc during aging and degeneration: comparison of surgical and control specimens. Spine 27: 798-805. doi:10.1097/00007632-200204150-00004. PubMed: 11935100. (Pubitemid 34522243)
22. Antoniou J, Steffen T, Nelson F, Winterbottom N, Hollander AP et al. (1996) The human lumbar intervertebral disc: evidence for changes in the biosynthesis and denaturation of the extracellular matrix with growth, maturation, ageing, and degeneration. J Clin Invest 98: 996-1003. doi:10.1172/JCI118884. PubMed: 8770872. (Pubitemid 26293318)
23. Antoniou J, Goudsouzian NM, Heathfield TF, Winterbottom N, Steffen T et al. (1996) The human lumbar endplate. Evidence of changes in biosynthesis and denaturation of the extracellular matrix with growth, maturation, aging, and degeneration. Spine 21: 1153-1163. doi:10.1097/00007632-199605150-00006. PubMed: 8727189.
24. Gruber HE, Hanley EN Jr (2003) Recent advances in disc cell biology. Spine 28: 186-193. PubMed: 12544938. (Pubitemid 36110088)
25. Nerlich AG, Schaaf R, Wächli B, Boos N (2007) Temporospatial distribution of blood vessels in human lumbar intervertebral discs. Eur Spine J 16: 547-555. doi:10.1007/s00586-006-0213-x. PubMed: 16947015. (Pubitemid 46626250)
26. Rajasekaran S, Babu JN, Arun R, Armstrong BR, Shetty AP et al. (2004) ISSLS prize winner: a study of diffusion in human lumbar discs: a serial magnetic resonance imaging study documenting the influence of the endplate on diffusion in normal and degenerate discs. Spine 29: 2654-2667. doi:10.1097/01.brs.0000148014.15210.64. PubMed: 15564914. (Pubitemid 40353251)
27. Bibby SR, Urban JP (2004) Effect of nutrient deprivation on the viability of intervertebral disc cells. Eur Spine J 13: 695-701. doi:10.1007/s00586-003- 0616-x. PubMed: 15048560. (Pubitemid 40416836)
28. Selard E, Shirazi-Adl A, Urban JP (2003) Finite element Study of nutrient diffusion in the human intervertebral disc Spine 28: 1945-1953 (Pubitemid 37082658)
29. Soukane DM, Shirazi-Adl A, Urban JP (2007) Computation of coupled diffusion of oxygen, glucose and lactic acid in an intervertebral disc. J Biomech 40: 2645-2654 (Pubitemid 47238090)
30. Hastreiter D, Ozuna RM, Spector M (2001) Regional variations in certain cellular characteristics in human lumbar intervertebral discs, including the presence of a-smooth muscle actin. J Orthop Res 19: 597-604. doi:10.1016/S0736-0266(00)00069-3. PubMed: 11518268. (Pubitemid 32700974)
31. Roberts S, Evans EH, Kletsas D, Jaffray DC, Eisenstein SM (2006) Senescence in human intervertebral discs. Eur Spine J 15 (suppl 3): 312-316. doi:10.1007/s00586-006-0126-8.
32. Maroudas A, Stockwell RA, Nachemson A, Urban J (1975) Factors involved in the nutrition of the human lumbar intervertebral disc: cellularity and diffusion of glucose in vitro. J Anat 120: 113-130. PubMed: 1184452.
33. Zhao CQ, Zhang YH, Jiang SD, Li H, Jiang LS et al. (2011) ADAMTS-5 and intervertebral disc degeneration: the results of tissue immunohistochemistry and in vitro cell culture. J Orthop Res 29: 718-725. doi:10.1002/jor.21285. PubMed: 21437951.
34. Malfait AM, Liu RQ, Ijiri K, Komiya S, Tortorella MD (2002) Inhibition of ADAM-TS4 and ADAM-TS5 prevents aggrecan degradation in osteoarthritic cartilage. J Biol Chem 277: 22201-22208. doi:10.1074/jbc.M200431200. PubMed: 11956193. (Pubitemid 34967185)
35. Naito S, Shiomi T, Okada A, Kimura T, Chijiiwa M et al. (2007) Expression of ADAMTS4 (aggrecanase-1) in human osteoarthritic cartilage. Pathol Int 57: 703-711. doi:10.1111/j.1440-1827.2007.02167.x. PubMed: 17922681. (Pubitemid 47537631)
36. Wang ZH, Yang ZQ, He XJ, Wang L, Li LX et al. (2008) Effects of RNAi-mediated inhibition of aggrecanase-1 and aggrecanase-2 on rat costochondral chondrocytes in vitro. Acta Pharmacol Sin 29: 1215-1226. doi:10.1111/j.1745- 7254.2008.00856.x. PubMed: 18817627.
37. Wang ZH, Yang ZQ, He XJ, Kamal BE, Xing Z (2010) Lentivirus mediated knockdown of aggrecanase-1 and -2 promotes chondrocyte-engineered cartilage formation in vitro. Biotechnol Bioeng 107: 730-736. doi:10.1002/bit.22862. PubMed: 20632367.
38. Seki S, Asanuma-Abe Y, Masuda K, Kawaguchi Y, Asanuma K et al. (2009) Effect of small interference RNA (siRNA) for ADAMTS5 on intervertebral disc degeneration in the rabbit anular needle-puncture model. Arthritis Res Ther 11: R166. doi:10.1186/ar2851. PubMed: 19889209.
39. Roberts S, Caterson B, Menage J, Evans EH, Jaffray DC et al. (2000) Matrix metalloproteinases and aggrecanase: their role in disorders of the human intervertebral disc. Spine 25: 3005-3013. doi:10.1097/00007632-200012010-00007. PubMed: 11145811.
40. Kanemoto M, Hukuda S, Komiya Y, Katsuura A, Nishioka J (1996) Immunohistochemical study of matrix metalloproteinase-3 and tissue inhibitor of metalloproteinase-1 human intervertebral discs. Spine 21: 1-8. doi:10.1097/00007632-199601010-00001. PubMed: 9122749. (Pubitemid 26031825)
41. Wallach CJ, Sobajima S, Watanabe Y, Kim JS, Georgescu HI et al. (2003) Gene transfer of the catabolic inhibitor TIMP-1 increases measured proteoglycans in cells from degenerated human intervertebral discs. Spine 28: 2331-2337. doi:10.1097/01.BRS.0000085303.67942.94. PubMed: 14560079. (Pubitemid 37271328)
42. Le Maitre CL, Freemont AJ, Hoyland JA (2005) The role of interleukin-1 in the pathogenesis of human intervertebral disc degeneration. Arthritis Res Ther 7: R732-R745. doi:10.1186/ar1732. PubMed: 15987475.
43. Wang J, Markova D, Anderson DG, Zheng Z, Shapiro IM et al. (2011) TNF-α and IL-1β promote a disintegrin-like and metalloprotease with thrombospondin type I motif-5-mediated aggrecan degradation through syndecan-4 in intervertebral disc. J Biol Chem 286: 39738-39749. doi:10.1074/jbc.M111. 264549. PubMed: 21949132.
44. Singh K, Masuda K, Thonar EJ, An HS, Cs-Szabo G (2009) Age-related changes in the extracellular matrix of nucleus pulposus and anulus fibrosus of human intervertebral disc. Spine 34: 10-16
45. Nerlich AG, Schleicher ED, Boos N (1997) 1997 Volvo Award winner in basic science studies. Immunohistologic markers for age-related changes of human lumbar intervertebral discs. Spine 22: 2781-2795. doi:10.1097/00007632- 199712150-00001. PubMed: 9431614. (Pubitemid 28070623)
46. Masuda K, Oegema TR Jr, An HS (2004) Growth factors and treatment of intervertebral disc degeneration. Spine 29: 2757-2769. Available online at: doi:10.1097/01.brs.0000146048.14946.af. PubMed: 15564925.
47. Tolonen J, Grönblad M, Vanharanta H, Virri J, Guyer RD et al. (2006) Growth factor expression in degenerated intervertebral disc tissue. An immunohistochemical analysis of transforming growth factor beta, fibroblast growth factor and platelet-derived growth factor. Eur Spine J 15: 588-596. doi:10.1007/s00586-005-0930-6. PubMed: 15980999.