An amino acid depleted cell-free protein synthesis system for the incorporation of non-canonical amino acid analogs into proteins

Journal of Biotechnology

Volumn 178, Issue 1, 2014, Pages 12-22

  Singh Blom, Amrita ^a   Hughes, Randall A ^a   Ellington, Andrew D ^a  

^a UNIVERSITY OF TEXAS AT AUSTIN   (United States)

Author keywords

Cell free protein synthesis; Residue specific incorporation; Streptavidin; Tryptophan; Unnatural amino acids

Indexed keywords

BIOSYNTHESIS; PROTEINS; CELLS; CYTOLOGY;

CELL FREE PROTEIN SYNTHESIS; RESIDUE-SPECIFIC INCORPORATION; STREPTAVIDIN; TRYPTOPHAN; UNNATURAL AMINO ACIDS;

AMINO ACIDS;

5 FLUOROTRYPTOPHAN; 6 FLUOROTRYPTOPHAN; AMINO ACID DERIVATIVE; AMINO ACID TRANSFER RNA LIGASE; BIOTIN; FLUORESCEIN ISOTHIOCYANATE; METHIONINE; RNA POLYMERASE; STREPTAVIDIN; TRYPTOPHAN DERIVATIVE; TRYPTOPHAN TRANSFER RNA LIGASE; UNCLASSIFIED DRUG; 5 METHYLTRYPTOPHAN; AMINO ACID; PROTEIN; TRYPTOPHAN; 5-FLUOROTRYPTOPHAN; 6-FLUOROTRYPTOPHAN;

AMINO ACID METABOLISM; ANIMAL CELL; ARTICLE; AUXOTROPHY; BACTERIAL CELL; CELL FREE SYSTEM; CELL LYSATE; DNA PROTEIN COMPLEX; DNA TEMPLATE; ESCHERICHIA COLI; HIGH THROUGHPUT SCREENING; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN QUATERNARY STRUCTURE; PROTEIN SYNTHESIS; REAL TIME POLYMERASE CHAIN REACTION; RESIDUE SPECIFIC INCORPORATION; WESTERN BLOTTING; AMINO ACID SUBSTITUTION; CONTROLLED STUDY; ECONOMIC ASPECT; IN VITRO STUDY; IN VIVO STUDY; MASS SPECTROMETRY; PRODUCTIVITY; PROTEIN ANALYSIS; PROTEIN BINDING; PROTEIN STRUCTURE; QUANTITATIVE ANALYSIS; ANALOGS AND DERIVATIVES; CHEMICAL STRUCTURE; CHEMISTRY; METABOLISM; PHYSIOLOGY;

BIOTIN; CELL-FREE SYSTEM; ESCHERICHIA COLI; MODELS, MOLECULAR; PROTEIN BIOSYNTHESIS; PROTEINS; STREPTAVIDIN; TRYPTOPHAN;

EID: 84896454374     PISSN: 01681656     EISSN: 18734863     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2014.02.009     Document Type: Article

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