Electrophoretic behavior of streptavidin complexed to a biotinylated probe: A functional screening assay for biotin-binding proteins

Electrophoresis

Volumn 26, Issue 1, 2005, Pages 47-52

  Humbert, Nicolas ^a   Zocchi, Andrea ^a   Ward, Thomas R ^a  

^a UNIVERSITY OF NEUCHÂTEL   (Switzerland)

Author keywords

Biotin 4 fluorescein; Functional screening; Nondenaturing sodium dodecyl sulfate polyacrylamide gel electrophoresis; Streptavidin

Indexed keywords

COENZYMES; ELECTROPHORESIS; SODIUM DODECYL SULFATE; SULFUR COMPOUNDS;

BINDING PROTEINS; BIOTIN BINDING; BIOTIN-4-FLUORESCEIN; ELECTROPHORETIC BEHAVIOR; FUNCTIONAL SCREENING; NONDENATURING SODIUM DODECYL SULPHATE-POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN STREPTAVIDIN; SCREENING ASSAYS; SODIUM DODECYL SULFATE-POLYACRYLAMIDE GEL ELECTROPHORESIS; STREPTAVIDIN;

PROBES;

ANAZOLENE SODIUM; BINDING PROTEIN; BIOTIN; BIOTIN DERIVATIVE; DODECYL SULFATE SODIUM; FLUORESCEIN DERIVATIVE; FLUORESCENT DYE; MUTANT PROTEIN; STREPTAVIDIN;

ARTICLE; BIOTINYLATION; COMPLEX FORMATION; CONTROLLED STUDY; DIAGNOSTIC IMAGING; HIGH TEMPERATURE PROCEDURES; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN DETERMINATION; PROTEIN FUNCTION; PROTEIN STABILITY; SCREENING TEST; ULTRAVIOLET RADIATION;

EID: 13444251466     PISSN: 01730835     EISSN: None     Source Type: Journal    
DOI: 10.1002/elps.200406148     Document Type: Article

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