High-throughput ClonePix FL analysis of mAb-expressing clones using the UCOE expression system

New Biotechnology

Volumn 31, Issue 3, 2014, Pages 214-220

  Hou, Jeff Jia Cheng ^a   Hughes, Ben S ^a,d   Smede, Matthew ^a   Leung, Kar Man ^a   Levine, Kara ^b   Rigby, Susan ^b   Gray, Peter P ^a   Munro, Trent P ^a,c  

^a UNIVERSITY OF QUEENSLAND   (Australia)

^b Pharm Chemical Solution (PCS) EMD Millipore   (United States)

^c AMGEN INC   (United States)

^d DSM RESOLVE   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

CELL CULTURE; CHROMOSOMES; CLONING; GENE EXPRESSION; MAMMALS; MONOCLONAL ANTIBODIES; PRODUCTIVITY; ROBOTICS;

CHINESE HAMSTER OVARY CELLS; CHROMOSOMAL INTEGRATION; EXPRESSION SYSTEM; EXPRESSION VECTORS; GENE AMPLIFICATION; GENETIC INSTABILITY; RECOMBINANT MONOCLONAL ANTIBODY; SPECIFIC PRODUCTIVITY;

CLONE CELLS;

IMMUNOGLOBULIN G; MONOCLONAL ANTIBODY; CHROMATIN;

ANALYTIC METHOD; ANIMAL CELL; ANIMAL EXPERIMENT; ANTIBODY PRODUCTION; ARTICLE; CELL CLONE; CELL ISOLATION; CELL LINE; CELL MATURATION; CELLULAR PARAMETERS; CHO CELL; CHROMATIN; EXPRESSION VECTOR; GENE AMPLIFICATION; GENE EXPRESSION; GENE EXPRESSION SYSTEM; GENETIC TRANSFECTION; GENETIC VARIABILITY; HIGH THROUGHPUT CLONEPIX FL; IMAGE ANALYSIS; MAMMAL CELL; NONHUMAN; PRIORITY JOURNAL; ROBOTICS; TRANSGENE; UBIQUITOUS CHROMATIN OPENING ELEMENT; ANIMAL; BATCH CELL CULTURE; CHO CELL LINE; CRICETULUS; GENE VECTOR; GENETICS; GUINEA PIG; HAMSTER; HIGH THROUGHPUT SCREENING; HUMAN; METABOLISM; NUCLEOTIDE SEQUENCE; PROCEDURES; PROMOTER REGION;

ANIMALS; ANTIBODIES, MONOCLONAL; BASE SEQUENCE; BATCH CELL CULTURE TECHNIQUES; CHO CELLS; CHROMATIN; CLONE CELLS; CRICETINAE; CRICETULUS; GENETIC VECTORS; GUINEA PIGS; HIGH-THROUGHPUT SCREENING ASSAYS; HUMANS; IMMUNOGLOBULIN G; PROMOTER REGIONS, GENETIC; TRANSFECTION;

EID: 84896078504     PISSN: 18716784     EISSN: 18764347     Source Type: Journal    
DOI: 10.1016/j.nbt.2014.02.002     Document Type: Article

References (37)

1. Intelligence L.M.B. Top 30 biologics 2010. R&D Pipeline News 2011.
2. Reichert J.M. Metrics for antibody therapeutics development. mAbs 2010, 2:695-700.
3. Aggarwal S. What's fueling the biotech engine - 2009-2010. Nature Biotechnology 2010, 28:1165-1171.
4. Walsh G. Biopharmaceutical benchmarks 2010. Nature Biotechnology 2010, 28:917-924.
5. Barnes L.M., Dickson A.J. Mammalian cell factories for efficient and stable protein expression. Current Opinion in Biotechnology 2006, 17:381-386.
6. Wurm F.M. Production of recombinant protein therapeutics in cultivated mammalian cells. Nature Biotechnology 2004, 22:1393-1398.
7. Kelley B. Industrialization of mAb production technology: the bioprocessing industry at a crossroads. mAbs 2009, 1:443-452.
8. Cockett M.I., Bebbington C.R., Yarranton G.T. High level expression of tissue inhibitor of metalloproteinases in Chinese hamster ovary cells using glutamine synthetase gene amplification. Biotechnology 1990, 8:662-667.
9. Kaufman R.J., Schimke R.T. Amplification and loss of dihydrofolate reductase genes in a Chinese hamster ovary cell line. Molecular and Cellular Biology 1981, 1:1069-1076.
10. Kaufman R.J. Selection and coamplification of heterologous genes in mammalian cells. Methods in Enzymology 1990, 185:537-566.
11. Lee G.M., Kim N.S., Kim S.J. Clonal variability within dihydrofolate reductase-mediated gene amplified Chinese hamster ovary cells: stability in the absence of selective pressure. Biotechnology and Bioengineering 1998, 60:679-688.
12. Pallavicini M.G., DeTeresa P.S., Rosette C., Gray J.W., Wurm F.M. Effects of methotrexate on transfected DNA stability in mammalian cells. Molecular and Cellular Biology 1990, 10:401-404.
13. Barnes L.M., Bentley C.M., Dickson A.J. Stability of protein production from recombinant mammalian cells. Biotechnology and Bioengineering 2003, 81:631-639.
14. Pilbrough W., Munro T.P., Gray P. Intraclonal protein expression heterogeneity in recombinant CHO cells. PLoS ONE 2009, 4:e8432.
15. Lee G.M., Kim S.J., Kim N.S., Ryu C.J., Hong H.J. Characterization of chimeric antibody producing CHO cells in the course of dihydrofolate reductase-mediated gene amplification and their stability in the absence of selective pressure. Biotechnology and Bioengineering 1998, 58:73-84.
16. Browne S.M., Al-Rubeai M. Selection methods for high-producing mammalian cell lines. Trends in Biotechnology 2007, 25:425-432.
17. Hacker D.L., De Jesus M., Wurm F.M. 25 years of recombinant proteins from reactor-grown cells - where do we go from here?. Biotechnology Advances 2009, 27:1023-1027.
18. Lindgren K., Salmen A., Lundgren M., Bylund L., Ebler A., Faldt E., et al. Automation of cell line development. Cytotechnology 2009, 59:1-10.
19. Caron A.W., Nicolas C., Gaillet B., Ba I., Pinard M., Garnier A., et al. Fluorescent labeling in semi-solid medium for selection of mammalian cells secreting high-levels of recombinant proteins. BMC Biotechnology 2009, 9:42.
20. Clarke J., Porter A., Davis J.M. Cloning. Animal cell culture 2011, 231-254. John Wiley & Sons, Ltd.
21. McKeehan W.L., Barnes D., Reid L., Stanbridge E., Murakami H., Sato G.H. Frontiers in mammalian cell culture. In Vitro Cellular and Developmental Biology 1990, 26:9-23.
22. Brezinsky S.C., Chiang G.G., Szilvasi A., Mohan S., Shapiro R.I., MacLean A., et al. A simple method for enriching populations of transfected CHO cells for cells of higher specific productivity. Journal of Immunological Methods 2003, 277:141-155.
23. Sleiman R.J., Gray P.P., McCall M.N., Codamo J., Sunstrom N.A. Accelerated cell line development using two-color fluorescence activated cell sorting to select highly expressing antibody-producing clones. Biotechnology and Bioengineering 2008, 99:578-587.
24. Serpieri F., Inocencio A., de Oliveira J.M., Pimenta A.A., Garbuio A., Kalil J., et al. Comparison of humanized IgG and FvFc anti-CD3 monoclonal antibodies expressed in CHO cells. Molecular Biotechnology 2010, 45:218-225.
25. Estes S., Melville M. Mammalian cell line developments in speed and efficiency. Advances in biochemical engineering/biotechnology 2014, 139:11-13.
26. Jostock T., Knopf H.P. Mammalian stable expression of biotherapeutics. Methods in Molecular Biology 2012, 899:227-238.
27. Crawford Y., Zhou M., Hu Z., Joly J., Snedecor B., Shen A., et al. Fast identification of reliable hosts for targeted cell line development from a limited-genome screening using combined phiC31 integrase and CRE-Lox technologies. Biotechnology Progress 2013, 29:1307-1315.
28. Turan S., Zehe C., Kuehle J., Qiao J., Bode J. Recombinase-mediated cassette exchange (RMCE) - a rapidly-expanding toolbox for targeted genomic modifications. Gene 2013, 515:1-27.
29. Kwaks T.H., Otte A.P. Employing epigenetics to augment the expression of therapeutic proteins in mammalian cells. Trends in Biotechnology 2006, 24:137-142.
30. Aldrich T., Thomas J., Morris A. Improved bicistronic mammalian expression vectors using expression augmenting sequence element (EASE). Cytotechnology 1998, 28:9-17.
31. Benton T., Chen T., McEntee M., Fox B., King D., Crombie R., et al. The use of UCOE vectors in combination with a preadapted serum free, suspension cell line allows for rapid production of large quantities of protein. Cytotechnology 2002, 38:43-46.
32. Boscolo S., Mion F., Licciulli M., Macor P., De Maso L., Brce M., et al. Simple scale-up of recombinant antibody production using an UCOE containing vector. New Biotechnology 2012, 29:477-484.
33. Antoniou M., Harland L., Mustoe T., Williams S., Holdstock J., Yague E., et al. Transgenes encompassing dual-promoter CpG islands from the human TBP and HNRPA2B1 loci are resistant to heterochromatin-mediated silencing. Genomics 2003, 82:269-279.
34. Williams S., Mustoe T., Mulcahy T., Griffiths M., Simpson D., Antoniou M., et al. CpG-island fragments from the HNRPA2B1/CBX3 genomic locus reduce silencing and enhance transgene expression from the hCMV promoter/enhancer in mammalian cells. BMC Biotechnology 2005, 5:17.
35. Nair A.R., Jinger X., Hermiston T.W. Effect of different UCOE-promoter combinations in creation of engineered cell lines for the production of Factor VIII. BMC Research Notes 2011, 4:178.
36. Ye J., Alvin K., Latif H., Hsu A., Parikh V., Whitmer T., et al. Rapid protein production using CHO stable transfection pools. Biotechnology Progress 2010, 26:1431-1437.
37. Palazzoli F., Bire S., Bigot Y., Bonnin-Rouleux F. Landscape of chromatin control element patents: positioning effects in pharmaceutical bioproduction. Nature Biotechnology 2011, 29:593-597.