Accelerated cell line development using two-color fluorescence activated cell sorting to select highly expressing antibody-producing clones

Biotechnology and Bioengineering

Volumn 99, Issue 3, 2008, Pages 578-587

  Sleiman, Robert J ^a   Gray, Peter P ^a   McCall, Martin N ^a   Codamo, Joe ^a   Sunstrom, Noelle Ann S ^a  

^a UNIVERSITY OF NEW SOUTH WALES   (Australia)

Author keywords

CHO; Fluorescence activated cell sorting; GFP; Recombinant antibody; Selection

Indexed keywords

CLONING; FLUORESCENCE SPECTROSCOPY; MONOCLONAL ANTIBODIES; PROTEINS;

BIOPHARMACEUTICALS; CELL LINE DEVELOPMENT; RECOMBINANT ANTIBODY;

CELLS;

RECOMBINANT ANTIBODY; MONOCLONAL ANTIBODY;

ANIMAL CELL; ANTIBODY PRODUCTION; ANTIGEN EXPRESSION; ARTICLE; CELL CLONE; CELL ISOLATION; CELL LINE; CELL SELECTION; CELL VIABILITY; CHINESE HAMSTER; CHO CELL; FLUORESCENCE ACTIVATED CELL SORTING; FLUORESCENCE ANALYSIS; GENETIC TRANSFECTION; NONHUMAN; ANIMAL; CELL SEPARATION; CRICETULUS; CULTURE TECHNIQUE; FLOW CYTOMETRY; HAMSTER; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; MOLECULAR CLONING; MULTIPHOTON MICROSCOPY;

CRICETULUS GRISEUS; MAMMALIA;

ANIMALS; ANTIBODIES, MONOCLONAL; CELL CULTURE TECHNIQUES; CELL SEPARATION; CHO CELLS; CLONING, MOLECULAR; CRICETINAE; CRICETULUS; FLOW CYTOMETRY; MICROSCOPY, FLUORESCENCE, MULTIPHOTON;

EID: 38449084043     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.21612     Document Type: Article

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