Fluorescent labeling in semi-solid medium for selection of mammalian cells secreting high-levels of recombinant proteins

BMC Biotechnology

Volumn 9, Issue , 2009, Pages

  Caron, Antoine W ^a   Nicolas, Claire ^a   Gaillet, Bruno ^a,b   Ba, Ismaïla ^a   Pinard, Maxime ^a   Garnier, Alain ^b   Massie, Bernard ^a,b,c   Gilbert, Rénald ^a,d  

^a NATIONAL RESEARCH COUNCIL CANADA   (Canada)

^b UNIVERSITÉ LAVAL   (Canada)

^c UNIVERSITÉ DE MONTRÉAL   (Canada)

^d MCGILL UNIVERSITY   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

BATCH CULTURE; CELL COLONIES; CELL SELECTION; CHINESE HAMSTER OVARY CELLS; CHO CELL; DILUTION METHOD; EFFICIENT METHOD; FLUORESCENT LABELING; FLUORESCENT SIGNALS; GREEN FLUORESCENT PROTEIN; HIGH THROUGHPUT; INSULIN-LIKE GROWTH FACTOR; LYMPHOID CELLS; MAMMALIAN CELLS; METHYLCELLULOSE; PLASMINOGEN ACTIVATORS; RECOMBINANT ANTIBODY; RECOMBINANT PROTEIN; SECONDARY ANTIBODIES; SECRETORS; SEMI-SOLID; TRANSFECTANTS; VERSATILE METHODS;

ANIMAL CELL CULTURE; BATCH CELL CULTURE; BIOACTIVITY; CELL CULTURE; CELLS; FLUORESCENCE; GENE EXPRESSION; INDUSTRIAL RESEARCH; LABELING; MAMMALS;

ANTIBODIES;

RECOMBINANT PROTEIN; SOMATOMEDIN; SOMATOMEDIN C; SOMATOMEDIN E5; UNCLASSIFIED DRUG; FLUORESCENT DYE; TISSUE PLASMINOGEN ACTIVATOR;

ANIMAL CELL; ARTICLE; CELL CLONE; CELL ISOLATION; CELL SECRETION; CELL SELECTION; CHO CELL; CONTROLLED STUDY; CULTURE MEDIUM; DILUTION; FLUORESCENCE ANALYSIS; FLUORESCENT LABELING IN SEMI SOLID MEDIUM; GENE EXPRESSION; INTERMETHOD COMPARISON; LYMPHOID CELL; MAMMAL CELL; MICROMANIPULATION; NONHUMAN; PROTEIN ANALYSIS; SCREENING TEST; ANIMAL; BIOSYNTHESIS; CELL SEPARATION; CRICETULUS; CULTURE TECHNIQUE; HAMSTER; METHODOLOGY;

CRICETULUS GRISEUS; MAMMALIA;

ANIMALS; CELL CULTURE TECHNIQUES; CELL SEPARATION; CHO CELLS; CRICETINAE; CRICETULUS; CULTURE MEDIA; FLUORESCENT DYES; INSULIN-LIKE GROWTH FACTOR I; RECOMBINANT PROTEINS; TISSUE PLASMINOGEN ACTIVATOR;

EID: 66749175713     PISSN: None     EISSN: 14726750     Source Type: Journal    
DOI: 10.1186/1472-6750-9-42     Document Type: Article

References (35)

1. Wurm FM Production of recombinant protein therapeutics in cultivated mammalian cells Nat Biotechnol 2004, 22:1393-1398. 10.1038/nbt1026 15529164
2. Butler M Animal cell cultures: Recent achievements and perspectives in the production of biopharmaceuticals Appl Microbiol Biotechnol 2005, 68:283-291. 10.1007/s00253-005-1980-8 15834715
3. Dingermann T Recombinant therapeutic proteins: Production platforms and challenges Biotechnol J 2008, 3:90-97. 10.1002/biot.200700214 18041103
4. Dinnis DM James DC Engineering mammalian cell factories for improved recombinant monoclonal antibody production: Lessons from nature? Biotechnol Bioeng 2005, 91:180-189. 10.1002/bit.20499 15880827
5. Browne SM Al-Rubeai M Selection methods for high-producing mammalian cell lines Trends Biotechnol 2007, 25:425-432. 10.1016/ j.tibtech.2007.07.002 17659798
6. Mosser DD Caron AW Bourget L Jolicoeur P Massie B Use of a dicistronic expression cassette encoding the green fluorescent protein for the screening and selection of cells expressing inducible gene products Biotechniques 1997, 22:150-161. 8994662
7. Meng YG Liang J Wong WL Chisholm V Green fluorescent protein as a second selectable marker for selection of high producing clones from transfected CHO cells Gene 2000, 242:201-207. 10.1016/ S0378-1119(99)00524-7 10721713
8. Mancia F Patel SD Rajala MW Scherer PE Nemes A Schieren I Hendrickson WA Shapiro L Optimization of protein production in mammalian cells with a coexpressed fluorescent marker Structure 2004, 12:1355-1360. 10.1016/ j.str.2004.06.012 15296729
9. Caron AW Massie B Mosser DD Use of a micromanipulator for high-efficiency cloning of cells co- expressing fluorescent proteins Methods Cell Sci 2000. 22:137-145. 10.1023/A:1009871029495 11264945
10. Sleiman RJ Gray PP McCall MN Codamo J Sunstrom NA Accelerated cell line development using two-color fluorescence activated cell sorting to select highly expressing antibody-producing clones Biotechnol Bioeng 2008, 99:578-587. 10.1002/bit.21612 17680677
11. DeMaria CT Cairns V Schwarz C Zhang J Guerin M Zuena E Estes S Karey KP Accelerated clone selection for recombinant CHO CELLS using a FACS-based high-throughput screen Biotechnol Prog 2007. 23:465-472. 10.1021/ bp060298i 17261021
12. Coffino P Scharff MD Rate of somatic mutation in immunoglobulin production by mouse myeloma cells Proc Natl Acad Sci USA 1971, 68:219-223. 391199 5276293 10.1073/pnas.68.1.219
13. Davis JM Pennington JE Kubler AM Conscience JF A simple, single-step technique for selecting and cloning hybridomas for the production of monoclonal antibodies J Immunol Methods 1982, 50:161-171. 10.1016/ 0022-1759(82)90222-8 7086152
14. Weaver JC McGrath P Adams S Gel microdrop technology for rapid isolation of rare and high producer cells Nat Med 1997, 3:583-585. 10.1038/ nm0597-583 9142132
15. Holmes P Al-Rubeai M Improved cell line development by a high throughput affinity capture surface display technique to select for high secretors J Immunol Methods 1999, 230:141-147. 10.1016/S0022-1759(99)00181-7 10594361
16. Borth N Zeyda M Kunert R Katinger H Efficient selection of high-producing subclones during gene amplification of recombinant Chinese hamster ovary cells by flow cytometry and cell sorting Biotechnol Bioeng 2000, 71:266-273. 10.1002/ 1097-0290(2000)71:4<266::AID-BIT1016>3.0.CO;2-2 11291036
17. Hanania EG Fieck A Stevens J Bodzin LJ Palsson BO Koller MR Automated in situ measurement of cell-specific antibody secretion and laser-mediated purification for rapid cloning of highly-secreting producers Biotechnol Bioeng 2005, 91:872-876. 10.1002/bit.20559 15937942
18. Lee C Ly C Sauerwald T Kelly T Moore G High-throughput screening of cell lines expressing monoclonal antibodies Bioprocess International 2006, 4(Suppl 3):32-35
19. Mann CJ Rapid isolation of antigen-specific clones from hybridoma fusions Nat Meth 2007, 4(4):i-ii
20. Massie B Couture F Lamoureux L Mosser DD Guilbault C Jolicoeur P Belanger F Langelier Y Inducible overexpression of a toxic protein by an adenovirus vector with a tetracycline-regulatable expression cassette J Virol 1998, 72:2289-2296. 109527 9499088
21. Kaufmann H Mazur X Fussenegger M Bailey JE Influence of low temperature on productivity, proteome and protein phosphorylation of CHO cells Biotechnol Bioeng 1999, 63:573-582. 10.1002/ (SICI)1097-0290(19990605)63:5<573::AID-BIT7>3.0.CO;2-Y 10397813
22. Fogolin MB Wagner R Etcheverrigaray M Kratje R Impact of temperature reduction and expression of yeast pyruvate carboxylase on hGM-CSF-producing CHO cells J Biotechnol 2004, 109:179-191. 10.1016/ j.jbiotec.2003.10.035 15063626
23. Yoon SK Song JY Lee GM Effect of low culture temperature on specific productivity, transcription level, and heterogeneity of erythropoietin in Chinese hamster ovary cells Biotechnol Bioeng 2003, 82:289-298. 10.1002/bit.10566 12599255
24. Gaillet B Gilbert R Amziani R Guilbault C Gadoury C Caron AW Mullick A Garnier A Massie B High-Level Recombinant Protein Production in CHO Cells Using an Adenoviral Vector and the Cumate Gene-Switch Biotechnol Prog 2007, 23:200-209. 10.1021/bp060187j 17269689
25. Cote J Garnier A Massie B Kamen A Serum-free production of recombinant proteins and adenoviral vectors by 293SF-3F6 cells Biotechnol Bioeng 1998, 59:567-575. 10.1002/ (SICI)1097-0290(19980905)59:5<567::AID-BIT6>3.0.CO;2-8 10099373
26. Newman ENC Whitney D Rapid automated selection of mammalian cell colonies by cell surface protein expression Nat Meth 2007, 4(5):v-vi.
27. Brezinsky SC Chiang GG Szilvasi A Mohan S Shapiro RI MacLean A Sisk W Thill G A simple method for enriching populations of transfected CHO cells for cells of higher specific productivity J Immunol Methods 2003, 277:141-155. 10.1016/S0022-1759(03)00108-X 12799047
28. Dowd JE Kwok KE Piret JM Increased t-PA yields using ultrafiltration of an inhibitory product from CHO fed-batch culture Biotechnol Prog 2000, 16:786-794. 10.1021/bp000079b 11027171
29. Senger RS Karim MN Effect of shear stress on intrinsic CHO culture state and glycosylation of recombinant tissue-type plasminogen activator protein Biotechnol Prog 2003, 19:1199-1209. 10.1021/bp025715f 12892482
30. Urlinger S Baron U Thellmann M Hasan MT Bujard H Hillen W Exploring the sequence space for tetracycline-dependent transcriptional activators: novel mutations yield expanded range and sensitivity Proc Natl Acad Sci USA 2000, 97:7963-7968. 16653 10859354 10.1073/pnas.130192197
31. Mullick A Xu Y Warren R Koutroumanis M Guilbault C Broussau S Malenfant F Bourget L Lamoureux L Lo R Caron AW Pilotte A Massie B The cumate gene-switch: A system for regulated expression in mammalian cells BMC Biotechnol 2006, 6:43. 1654148 17083727 10.1186/1472-6750-6-43
32. Gervais C Paquette D Burns-Tardis A Martin L Massie B Development of high output expression vectors for antibody production in mammalian cells Animal Cell Technology: Basic & Applied Aspects Netherlands: Kluwer Academic Publishers Nagai K, Wachi M 1998:349-354
33. Zeng Y Pinard M Jaime J Bourget L Uyen LP O'Connor-McCourt MD Gilbert R Massie B A ligand-pseudoreceptor system based on de novo designed peptides for the generation of adenoviral vectors with altered tropism J Gene Med 2008, 10:355-367. 10.1002/jgm.1155 18189335
34. De Crescenzo G Litowski JR Hodges RS O'Connor-McCourt MD Real-time monitoring of the interactions of two-stranded de novo designed coiled-coils: Effect of chain length on the kinetic and thermodynamic constants of binding Biochemistry 2003, 42:1754-1763. 10.1021/bi0268450 12578390
35. Gingrich JC Davis DR Nguyen Q Multiplex detection and quantitation of proteins on western blots using fluorescent probes Biotechniques 2000, 29:636-642. 10997278