Small molecules enable cardiac reprogramming of mouse fibroblasts with a single factor, oct4

Cell Reports

Volumn 6, Issue 5, 2014, Pages 951-960

  Wang, Haixia ^a,b   Cao, Nan ^a,b   Spencer, C Ian ^a,b   Nie, Baoming ^a,b   Ma, Tianhua ^a,b   Xu, Tao ^a,b   Zhang, Yu ^a,b   Wang, Xiaojing ^a,b   Srivastava, Deepak ^a,b,c   Ding, Sheng ^a,b,d  

^a GLADSTONE INSTITUTE OF CARDIOVASCULAR DISEASE   (United States)

^b Roddenberry Center for Stem Cell Biology and Medicine at Gladstone   (United States)

^c UNIVERSITY OF CALIFORNIA   (United States)

^d UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

4 [4 (1,3 BENZODIOXOL 5 YL) 5 (2 PYRIDINYL) 1H IMIDAZOL 2 YL]BENZAMIDE; FORSKOLIN; OCTAMER TRANSCRIPTION FACTOR 4; TRANYLCYPROMINE; 6 [[2 [[4 (2,4 DICHLOROPHENYL) 5 (4 METHYL 1H IMIDAZOL 2 YL) 2 PYRIMIDINYL]AMINO]ETHYL]AMINO]NICOTINONITRILE; MOLECULAR LIBRARY; POU5F1 PROTEIN, MOUSE;

ANIMAL CELL; ARTICLE; CONTROLLED STUDY; FIBROBLAST; HEART ELECTROPHYSIOLOGY; HEART MUSCLE CELL; HEART VENTRICLE; MOUSE; NONHUMAN; PLURIPOTENT STEM CELL; PRIORITY JOURNAL; ANIMAL TISSUE; CARDIAC STEM CELL; CELL DIFFERENTIATION; CELL LINEAGE; EMBRYO; MUSCLE FIBER CONTRACTION; NUCLEAR REPROGRAMMING; PHENOTYPE; ANIMAL; CELL CULTURE; CYTOLOGY; DRUG EFFECTS; GENETICS; METABOLISM; MOLECULAR LIBRARY; PHARMACOLOGY; PHYSIOLOGY;

ANIMALS; CELL DIFFERENTIATION; CELLS, CULTURED; CELLULAR REPROGRAMMING; FIBROBLASTS; MICE; MYOCYTES, CARDIAC; OCTAMER TRANSCRIPTION FACTOR-3; SMALL MOLECULE LIBRARIES;

EID: 84895921071     PISSN: None     EISSN: 22111247     Source Type: Journal    
DOI: 10.1016/j.celrep.2014.01.038     Document Type: Article

References (39)

1. Chambers S.M., Fasano C.A., Papapetrou E.P., Tomishima M., Sadelain M., Studer L. Highly efficient neural conversion of human ESand iPS cells by dual inhibition of SMAD signaling. Nat. Biotechnol. 2009, 27:275-280.
2. Do J.T., Schöler H.R. Nuclei of embryonic stem cells reprogram somatic cells. Stem Cells 2004, 22:941-949.
3. Efe J.A., Hilcove S., Kim J., Zhou H., Ouyang K., Wang G., Chen J., Ding S. Conversion of mouse fibroblasts into cardiomyocytes using a direct reprogramming strategy. Nat. Cell Biol. 2011, 13:215-222.
4. Han D.W., Tapia N., Hermann A., Hemmer K., Höing S., Araúzo-Bravo M.J., Zaehres H., Wu G., Frank S., Moritz S., et al. Direct reprogramming of fibroblasts into neural stem cells by defined factors. Cell Stem Cell 2012, 10:465-472.
5. Hanna J., Cheng A.W., Saha K., Kim J., Lengner C.J., Soldner F., Cassady J.P., Muffat J., Carey B.W., Jaenisch R. Human embryonic stem cells with biological and epigenetic characteristics similar to those of mouse ESCs. Proc. Natl. Acad. Sci. USA 2010, 107:9222-9227.
6. Hilcove, S.A. (2010). Small molecules, regeneration, and cell fate. PhD thesis. Scripps Research Institute, La Jolla, CA.
7. Ieda M., Tsuchihashi T., Ivey K.N., Ross R.S., Hong T.T., Shaw R.M., Srivastava D. Cardiac fibroblasts regulate myocardial proliferation through beta1 integrin signaling. Dev. Cell 2009, 16:233-244.
8. Ieda M., Fu J.D., Delgado-Olguin P., Vedantham V., Hayashi Y., Bruneau B.G., Srivastava D. Direct reprogramming of fibroblasts into functional cardiomyocytes by defined factors. Cell 2010, 142:375-386.
9. Kathiresan S., Srivastava D. Genetics of human cardiovascular disease. Cell 2012, 148:1242-1257.
10. Kattman S.J., Witty A.D., Gagliardi M., Dubois N.C., Niapour M., Hotta A., Ellis J., Keller G. Stage-specific optimization of activin/nodal and BMP signaling promotes cardiac differentiation of mouse and human pluripotent stem cell lines. Cell Stem Cell 2011, 8:228-240.
11. Kim J., Efe J.A., Zhu S., Talantova M., Yuan X., Wang S., Lipton S.A., Zhang K., Ding S. Direct reprogramming of mouse fibroblasts to neural progenitors. Proc. Natl. Acad. Sci. USA 2011, 108:7838-7843.
12. Korhonen T., Rapila R., Ronkainen V.-P., Koivumäki J.T., Tavi P. Local Ca2+ releases enable rapid heart rates in developing cardiomyocytes. J.Physiol. 2010, 588:1407-1417.
13. Kuzmenkin A., Liang H., Xu G., Pfannkuche K., Eichhorn H., Fatima A., Luo H., Saric T., Wernig M., Jaenisch R., Hescheler J. Functional characterization of cardiomyocytes derived from murine induced pluripotent stem cells invitro. FASEB J. 2009, 23:4168-4180.
14. Li W., Zhou H., Abujarour R., Zhu S., Young Joo J., Lin T., Hao E., Schöler H.R., Hayek A., Ding S. Generation of human-induced pluripotent stem cells in the absence of exogenous Sox2. Stem Cells 2009, 27:2992-3000.
15. Li R., Liang J., Ni S., Zhou T., Qing X., Li H., He W., Chen J., Li F., Zhuang Q., et al. A mesenchymal-to-epithelial transition initiates and is required for the nuclear reprogramming of mouse fibroblasts. Cell Stem Cell 2010, 7:51-63.
16. Li Y., Zhang Q., Yin X., Yang W., Du Y., Hou P., Ge J., Liu C., Zhang W., Zhang X., et al. Generation of iPSCs from mouse fibroblasts with a single gene, Oct4, and small molecules. Cell Res. 2011, 21:196-204.
17. Li J., Huang N.F., Zou J., Laurent T.J., Lee J.C., Okogbaa J., Cooke J.P., Ding S. Conversion of human fibroblasts to functional endothelial cells by defined factors. Arterioscler. Thromb. Vasc. Biol. 2013, 33:1366-1375.
18. Li K., Russ H.A., Xu S., Xu T., Zhang Y., Ma T., Hebrok M., Ding S. Small molecules facilitate the reprogramming of mouse fibroblasts into pancreatic lineages. Cell Stem Cell 2014, Published online February 6, 2014. 10.1016/j.stem.2014.01.006.
19. Lian X., Hsiao C., Wilson G., Zhu K., Hazeltine L.B., Azarin S.M., Raval K.K., Zhang J., Kamp T.J., Palecek S.P. Robust cardiomyocyte differentiation from human pluripotent stem cells via temporal modulation of canonical Wnt signaling. Proc. Natl. Acad. Sci. USA 2012, 109:E1848-E1857.
20. Lieu D.K., Liu J., Siu C.-W., McNerney G.P., Tse H.-F., Abu-Khalil A., Huser T., Li R.A. Absence of transverse tubules contributes tonon-uniform Ca(2+) wavefronts in mouse and human embryonic stem cell-derived cardiomyocytes. Stem Cells Dev. 2009, 18:1493-1500.
21. Lin T., Ambasudhan R., Yuan X., Li W., Hilcove S., Abujarour R., Lin X., Hahm H.S., Hao E., Hayek A., Ding S. A chemical platform for improved induction of human iPSCs. Nat. Methods 2009, 6:805-808.
22. Lu J., Liu H., Huang C.T.-L., Chen H., Du Z., Liu Y., Sherafat M.A., Zhang S.-C. Generation of integration-free and region-specific neural progenitors from primate fibroblasts. Cell reports 2013.
23. Muzumdar M.D., Tasic B., Miyamichi K., Li L., Luo L. A global double-fluorescent Cre reporter mouse. Genesis 2007, 45:593-605.
24. Naito A.T., Shiojima I., Akazawa H., Hidaka K., Morisaki T., Kikuchi A., Komuro I. Developmental stage-specific biphasic roles of Wnt/beta-catenin signaling in cardiomyogenesis and hematopoiesis. Proc. Natl. Acad. Sci. USA 2006, 103:19812-19817.
25. Nie B., Wang H., Laurent T., Ding S. Cellular reprogramming: a small molecule perspective. Curr. Opin. Cell Biol. 2012, 24:784-792.
26. Qian L., Huang Y., Spencer C.I., Foley A., Vedantham V., Liu L., Conway S.J., Fu J.D., Srivastava D. Invivo reprogramming of murine cardiac fibroblasts into induced cardiomyocytes. Nature 2012, 485:593-598.
27. Sancho-Martinez I., Baek S.H., Izpisua Belmonte J.C. Lineage conversion methodologies meet the reprogramming toolbox. Nat. Cell Biol. 2012, 14:892-899.
28. Sauer H., Theben T., Hescheler J., Lindner M., Brandt M.C., Wartenberg M. Characteristics of calcium sparks in cardiomyocytes derived from embryonic stem cells. Am. J. Physiol. Heart Circ. Physiol. 2001, 281:H411-H421.
29. Shi Y., Desponts C., Do J.T., Hahm H.S., Schöler H.R., Ding S. Induction of pluripotent stem cells from mouse embryonic fibroblasts by Oct4 and Klf4 with small-molecule compounds. Cell Stem Cell 2008, 3:568-574.
30. Song K., Nam Y.J., Luo X., Qi X., Tan W., Huang G.N., Acharya A., Smith C.L., Tallquist M.D., Neilson E.G., et al. Heart repair by reprogramming non-myocytes with cardiac transcription factors. Nature 2012, 485:599-604.
31. Srinivas S., Watanabe T., Lin C.S., William C.M., Tanabe Y., Jessell T.M., Costantini F. Cre reporter strains produced by targeted insertion of EYFP and ECFP into the ROSA26 locus. BMC Dev. Biol. 2001, 1:4.
32. Takamatsu H., Nagao T., Ichijo H., Adachi-Akahane S. L-type Ca2+ channels serve as a sensor of the SR Ca2+ for tuning the efficacy of Ca2+-induced Ca2+ release in rat ventricular myocytes. J.Physiol. 2003, 552:415-424.
33. Thier M., Wörsdörfer P., Lakes Y.B., Gorris R., Herms S., Opitz T., Seiferling D., Quandel T., Hoffmann P., Nöthen M.M., et al. Direct conversion of fibroblasts into stably expandable neural stem cells. Cell Stem Cell 2012, 10:473-479.
34. Willems E., Cabral-Teixeira J., Schade D., Cai W., Reeves P., Bushway P.J., Lanier M., Walsh C., Kirchhausen T., Izpisua Belmonte J.C., et al. Small molecule-mediated TGF-β type II receptor degradation promotes cardiomyogenesis in embryonic stem cells. Cell Stem Cell 2012, 11:242-252.
35. Yang L., Soonpaa M.H., Adler E.D., Roepke T.K., Kattman S.J., Kennedy M., Henckaerts E., Bonham K., Abbott G.W., Linden R.M., et al. Human cardiovascular progenitor cells develop from a KDR+ embryonic-stem-cell-derived population. Nature 2008, 453:524-528.
36. Yuan X., Wan H., Zhao X., Zhu S., Zhou Q., Ding S. Brief report: combined chemical treatment enables Oct4-induced reprogramming from mouse embryonic fibroblasts. Stem Cells 2011, 29:549-553.
37. Zhu S., Li W., Zhou H., Wei W., Ambasudhan R., Lin T., Kim J., Zhang K., Ding S. Reprogramming of human primary somatic cells by OCT4 and chemical compounds. Cell Stem Cell 2010, 7:651-655.
38. Zhu S., Wei W., Ding S. Chemical strategies for stem cell biology and regenerative medicine. Annu. Rev. Biomed. Eng. 2011, 13:73-90.
39. Zhu S., Ambasudhan R., Sun W., Kim H.J., Talantova M., Wang X., Zhang M., Zhang Y., Laurent T., Parker J., et al. Small molecules enable OCT4-mediated direct reprogramming into expandable human neural stem cells. Cell Res. 2014, 24:126-129.