A novel truncated form of eNOS associates with altered vascular function

Cardiovascular Research

Volumn 101, Issue 3, 2014, Pages 492-502

  Galluccio, Elena ^a   Cassina, Laura ^b   Russo, Isabella ^c   Gelmini, Fabrizio ^d   Setola, Emanuela ^a   Rampoldi, Luca ^a,e   Citterio, Lorena ^a   Rossodivita, Alessandra ^a   Kamami, Mikel ^a   Colombo, Antonio ^a   Alfieri, Ottavio ^a,b   Carini, Marina ^d   Bosi, Emanuele ^a,b   Trovati, Mariella ^c   Piatti, Piermarco ^a   Monti, Lucilla D ^a   Casari, Giorgio ^b  

^a SAN RAFFAELE HOSPITAL   (Italy)

^b VITA SALUTE SAN RAFFAELE UNIVERSITY   (Italy)

^c UNIVERSITY OF TURIN   (Italy)

^d UNIVERSITY OF MILAN   (Italy)

^e DULBECCO TELETHON INSTITUTE   (Italy)

Author keywords

Endothelial function; eNOS; Nitric oxide; Novel isoform

Indexed keywords

ENDOTHELIAL FUNCTION; ENOS; NITRIC OXIDE; NOVEL ISOFORM;

ADULT; AGED; CORONARY ARTERY DISEASE; ENDOTHELIUM, VASCULAR; GENE FREQUENCY; GENETIC PREDISPOSITION TO DISEASE; GENETIC TESTING; GENOTYPE; HUMANS; MIDDLE AGED; NITRIC OXIDE SYNTHASE TYPE III; POLYMORPHISM, GENETIC; VASODILATION;

EID: 84894574194     PISSN: 00086363     EISSN: 17553245     Source Type: Journal    
DOI: 10.1093/cvr/cvt267     Document Type: Article

References (36)

1. Sessa WC, Harrison JK, Barber CM, Zeng D, Durieux ME, D'AngeloDDet al. Molecular cloning and expression of a cDNA encoding endothelial cell nitric oxide synthase. J Biol Chem 1992;267:15274-15276.
2. Chen PF, Tsai AL, Berka V, WuKK. Endothelial nitric-oxide synthase. Evidence for bidomain structure and successful reconstitution of catalytic activity from two separate domains generated by a baculovirus expression system. J Biol Chem 1996;271: 14631-14635.
3. Huang PL, Huang Z, MashimoH, BlochKD, Moskowitz MA, Bevan JA et al. Hypertension in mice lacking the gene for endothelial nitric oxide synthase. Nature 1995;377:239-242.
4. Ohashi Y, Kawashima S, Hirata K, Yamashita T, Ishida T, Inoue N et al. Hypotension and reduced nitric oxide-elicited vasorelaxation in transgenic mice overexpressing endothelial nitric oxide synthase. J Clin Invest 1998;102:2061-2071.
5. Quyyumi AA, Dakak N, Andrews NP, Husain S, Arora S, Gilligan DM et al. Nitric oxide activity in the human coronary circulation. Impact of risk factors for coronary atherosclerosis. J Clin Invest 1995;95:1747-1755.
6. Oemar BS, Tschudi MR, Godoy N, Brovkovich V, Malinski T, Luscher TF. Reduced endothelial nitric oxide synthase expression and production in human atherosclerosis. Circulation 1998;97:2494-2498.
7. Clarkson P, Celermajer DS, Powe AJ, Donald AE, HenryRM, Deanfield JE. Endotheliumdependent dilatation is impaired in young healthy subjects with a family history of premature coronary disease. Circulation 1997;96:3378-3383.
8. SchachingerV, Britten MB, ElsnerM, Walter DH, Scharrer I, Zeiher AM.Apositive family history of premature coronary artery disease is associated with impaired endotheliumdependent coronary blood flow regulation. Circulation 1999;100:1502-1508.
9. O'Donnell CJ, Cupples LA, D'Agostino RB, Fox CS, Hoffmann U, Hwang SJ et al. Genome-wide association study for subclinical atherosclerosis in major arterial territories in the NHLBI's Framingham Heart Study. BMC Med Genet 2007;8(Suppl 1):S4.
10. Johnson T, Gaunt TR, Newhouse SJ, Padmanabhan S, Tomaszewski M, Kumari M et al. Blood pressure loci identified with a gene-centric array. Am J Hum Genet 2011;89: 688-700.
11. Kuhlencordt PJ, Gyurko R, Han F, Scherrer-Crosbie M, Aretz TH, Hajjar R et al. Accelerated atherosclerosis, aortic aneurysm formation, and ischemic heart disease in apolipoprotein E/endothelial nitric oxide synthase double-knockout mice. Circulation 2001; 104:448-454.
12. Monti LD, Barlassina C, Citterio L, Galluccio E, Berzuini C, Setola E et al. Endothelial nitric oxide synthase polymorphisms are associated with type 2 diabetes and the insulin resistance syndrome. Diabetes 2003;52:1270-1275.
13. Galluccio E, Piatti P, Citterio L, Lucotti PC, Setola E, Cassina L et al. Hyperinsulinemia and impaired leptin-adiponectin ratio associate with endothelial nitric oxide synthase polymorphisms in subjects with in-stent restenosis. Am J Physiol Endocrinol Metab 2008;294: E978-E986.
14. Pagani F, Buratti E, Stuani C, Bendix R, DorkT, Baralle FE.Anewtypeof mutation causes a splicing defect in ATM. Nat Genet 2002;30:426-429.
15. Forstermann U, Sessa WC. Nitric oxide synthases: regulation and function. Eur Heart J 2012;33:829-837, 837a-837d.
16. Bevers LM, Braam B, Post JA, van Zonneveld AJ, Rabelink TJ, Koomans HA et al. Tetrahydrobiopterin, but not L-arginine, decreases NO synthase uncoupling in cells expressing high levels of endothelial NO synthase. Hypertension 2006;47:87-94.
17. Stuehr DJ, Tejero J, Haque MM.Structural and mechanistic aspects of flavoproteins: electron transfer through the nitric oxide synthase flavoprotein domain. FEBS J 2009;276: 3959-3974.
18. Daff S. NO synthase: structures and mechanisms. Nitric Oxide 2010;23:1-11.
19. Feng C, Taiakina V, Ghosh DK, Guillemette JG, Tollin G. Intraprotein electron transfer between the FMN and heme domains in endothelial nitric oxide synthase holoenzyme. Biochim Biophys Acta 2011;1814:1997-2002.
20. Ghosh DK, Holliday MA, Thomas C, Weinberg JB, Smith SM, Salerno JC. Nitric-oxide synthase output state. Design and properties of nitric-oxide synthase oxygenase/FMN domain constructs. J Biol Chem 2006;281:14173-14183.
21. Knudsen GM, Nishida CR, Mooney SD, Ortiz de Montellano PR. Nitric-oxide synthase (NOS) reductase domain models suggest anewcontrol element in endothelialNOSthat attenuates calmodulin-dependent activity. J Biol Chem 2003;278:31814-31824.
22. Fulton D, Gratton JP, McCabe TJ, Fontana J, Fujio Y, Walsh K et al. Regulation of endothelium-derived nitric oxide production by the protein kinase Akt. Nature 1999; 399:597-601.
23. Atochin DN, Wang A, LiuVW, Critchlow JD, Dantas AP, Looft-Wilson Ret al. The phosphorylation state of eNOS modulates vascular reactivity and outcome of cerebral ischemia in vivo. J Clin Invest 2007;117:1961-1967.
24. Ghosh S, Gachhui R, Crooks C, Wu C, Lisanti MP, Stuehr DJ. Interaction between caveolin-1 and the reductase domain of endothelial nitric-oxide synthase. Consequences for catalysis. J Biol Chem 1998;273:22267-22271.
25. Gusarov I, Starodubtseva M, Wang ZQ, McQuade L, Lippard SJ, Stuehr DJ et al. Bacterial nitric-oxide synthases operate without a dedicated redox partner. J Biol Chem 2008;283: 13140-13147.
26. Stuehr D, Pou S, Rosen GM. Oxygen reduction by nitric-oxide synthases. J Biol Chem 2001;276:14533-14536.
27. Lee CM, Robinson LJ, Michel T. Oligomerization of endothelial nitric oxide synthase. Evidence for a dominant negative effect of truncation mutants. J Biol Chem 1995;270: 27403-27406.
28. Lorenz M, Hewing B, Hui J, Zepp A, Baumann G, Bindereif A et al. Alternative splicing in intron 13 of the human eNOS gene: a potential mechanism for regulating eNOS activity. FASEB J 2007;21:1556-1564.
29. Zeng G, Nystrom FH, Ravichandran LV, Cong LN, Kirby M, Mostowski H et al. Roles for insulin receptor, PI3-kinase, and Akt in insulin-signaling pathways related to production of nitric oxide in human vascular endothelial cells. Circulation 2000;101:1539-1545.
30. AnfossiG, Russo I, Doronzo G, Trovati M.Relevanceof the vascular effects of insulin in the rationale of its therapeutical use. Cardiovasc Hematol Disord Drug Targets 2007;7:228-249.
31. Moncada S, Higgs EA. Endogenous nitric oxide: physiology, pathology and clinical relevance. Eur J Clin Invest 1991;21:361-374.
32. Tagawa T, Imaizumi T, Endo T, Shiramoto M, Harasawa Y, Takeshita A. Role of nitric oxide in reactive hyperemia in human forearm vessels. Circulation 1994;90:2285-2290.
33. WrayDW, WitmanMA, Ives SJ, McDaniel J, Trinity JD, Conklin JDet al.Doesbrachialartery flow-mediated vasodilation provide a bioassay for NO? Hypertension 2013;62:345-351.
34. Piatti PM, Marone E, Mantero M, Setola E, Galluccio E, Lucotti P et al. Effect of normalization of fasting glucose by intensified insulin therapy and influence of eNOS polymorphisms on the incidence of restenosis after peripheral angioplasty in patients with type 2 diabetes: a randomized, open-label clinical trial. Acta Diabetol 2012;50:373-82.
35. Antoniades C, Tousoulis D, Vasiliadou C, Pitsavos C, Toutouza M, Tentolouris C et al. Genetic polymorphisms G894T on the eNOS gene is associated with endothelial function and vWF levels in premature myocardial infarction survivors. Int J Cardiol 2006;107: 95-100.
36. Halcox JP, Donald AE, Ellins E, WitteDR, Shipley MJ, Brunner EJ et al. Endothelial function predicts progression of carotid intima-media thickness. Circulation 2009;119: 1005-1012.