Endothelial nitric-oxide synthase: Evidence for bidomain structure and successful reconstitution of catalytic activity from two separate domains generated by a baculovirus expression system

Journal of Biological Chemistry

Volumn 271, Issue 24, 1996, Pages 14631-14635

  Chen, Pei Feng ^a   Tsai, Ah Lim ^a   Berka, Vladimir ^a   Wu, Kenneth K ^a  

^a UNIVERSITY OF TEXAS MEDICAL SCHOOL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ORYCTOLAGUS CUNICULUS; UNIDENTIFIED BACULOVIRUS;

ARGININE; CALCIUM ION; CALMODULIN; CYTOCHROME C; FERROUS ION; HISTIDINE; IMMUNOGLOBULIN G; NITRIC OXIDE SYNTHASE; OXIDOREDUCTASE; OXYGENASE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE;

AMINO TERMINAL SEQUENCE; ARTICLE; BACULOVIRUS; CATALYSIS; CONTROLLED STUDY; ENDOTHELIUM; ENZYME ACTIVITY; HUMAN; HUMAN CELL; IMMUNOBLOTTING; IMMUNOREACTIVITY; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL;

ANIMALS; BACULOVIRIDAE; BASE SEQUENCE; BINDING SITES; BLOTTING, WESTERN; CALCIUM; CALMODULIN; CLONING, MOLECULAR; DNA PRIMERS; ENDOTHELIUM, VASCULAR; HISTIDINE; HUMANS; IMMUNOGLOBULIN G; KINETICS; MOLECULAR SEQUENCE DATA; NADPH-FERRIHEMOPROTEIN REDUCTASE; NITRIC OXIDE SYNTHASE; OXYGENASES; POLYMERASE CHAIN REACTION; RABBITS; RECOMBINANT PROTEINS; SEQUENCE TAGGED SITES; SPECTROPHOTOMETRY; SPODOPTERA; TRANSFECTION; TRYPSIN;

EID: 0030007024     PISSN: 00219258     EISSN: None     Source Type: Journal    
DOI: 10.1074/jbc.271.24.14631     Document Type: Article

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