Directed evolution of GH43 β-xylosidase XylBH43 thermal stability and L186 saturation mutagenesis

Journal of Industrial Microbiology and Biotechnology

Volumn 41, Issue 3, 2014, Pages 489-498

  Singh, Sanjay K ^b   Heng, Chamroeun ^a   Braker, Jay D ^c   Chan, Victor J ^a   Lee, Charles C ^a   Jordan, Douglas B ^c   Yuan, Ling ^b   Wagschal, Kurt ^a  

^a WESTERN REGIONAL RESEARCH CENTER   (United States)

^b UNIVERSITY OF KENTUCKY   (United States)

^c NATIONAL CENTER FOR AGRICULTURAL UTILIZATION RESEARCH   (United States)

Author keywords

Directed evolution; Gene shuffling; Glycosyl hydrolase; Protein engineering; Thermal stability

Indexed keywords

AMINO ACID SEQUENCE; BACILLUS; DIRECTED MOLECULAR EVOLUTION; DISACCHARIDES; ENZYME STABILITY; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTAGENESIS; NITROPHENOLS; SEQUENCE ALIGNMENT; SUBSTRATE SPECIFICITY; XYLOSE; XYLOSIDASES;

EID: 84894418601     PISSN: 13675435     EISSN: 14765535     Source Type: Journal    
DOI: 10.1007/s10295-013-1377-0     Document Type: Article

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