Enzyme-coupled assay for β-xylosidase hydrolysis of natural substrates

Applied and Environmental Microbiology

Volumn 71, Issue 9, 2005, Pages 5318-5323

  Wagschal, Kurt ^a   Franqui Espiet, Diana ^a   Lee, Charles C ^a   Robertson, George H ^a   Wong, Dominic W S ^a  

^a WESTERN REGIONAL RESEARCH CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CHROMOPHORES; ENZYME INHIBITION; ENZYME KINETICS; HYDROGEN PEROXIDE; HYDROLYSIS; POSITIVE IONS;

D-XYLOSE; HEMICELLULOLYTIC ENZYMES; XYLOOLIGOSACCHARIDE SUBSTRATES;

BIOASSAY;

2,2' AZINOBIS(3 ETHYLBENZOTHIAZOLINE 6 SULFONATE); ACETIC ACID DERIVATIVE; BENZOTHIAZOLINE DERIVATIVE; CATION; DIAMINOBENZIDINE; GLYCOSIDASE; HYDROGEN PEROXIDE; N ACETYL 3,7 DIHYDROXYPHENOXAZINE; OLIGOSACCHARIDE; PEROXIDASE; PHENOXAZINE DERIVATIVE; RADICAL; RESORUFIN; SULFONIC ACID DERIVATIVE; UNCLASSIFIED DRUG; XYLAN 1,4 BETA XYLOSIDASE; XYLOBIOSE; XYLOOLIGOSACCHARIDE; XYLOSE; XYLOTRIOSE;

ENZYME; HYDROLYSIS;

AMINO ACID SEQUENCE; ARTICLE; BACTERIAL STRAIN; CONCENTRATION (PARAMETERS); CURVE FITTING; ENZYME ASSAY; ENZYME KINETICS; ENZYME SUBSTRATE COMPLEX; HYDROLYSIS; NONHUMAN; PROTEIN FAMILY; QUANTITATIVE ASSAY; SPECTRAL SENSITIVITY; THERMOANAEROBACTERIACEAE;

BENZOTHIAZOLES; CHROMOGENIC COMPOUNDS; CULTURE MEDIA; DISACCHARIDES; ESCHERICHIA COLI; HYDROGEN PEROXIDE; HYDROLYSIS; KINETICS; OXAZINES; SUBSTRATE SPECIFICITY; SULFONIC ACIDS; THERMOANAEROBACTERIUM; XYLOSE; XYLOSIDASES;

BACTERIA (MICROORGANISMS); THERMOANAEROBACTERIACEAE; THERMOANAEROBACTERIUM SP.;

EID: 25144473735     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.71.9.5318-5323.2005     Document Type: Article

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