Characterization of charged functional domains introduced into a modified pectic homogalacturonan by an acidic plant pectin methylesterase (Ficus awkeotsang Makino) and modeling of enzyme mode of action

Food Hydrocolloids

Volumn 39, Issue , 2014, Pages 319-329

  Kim, Yang ^a   Williams, Martin A K ^b,c,d   Tzen, Jason T C ^e   Luzio, Gary A ^a   Galant, Ashley L ^a   Cameron, Randall G ^a  

^a U S HORTICULTURAL RESEARCH LABORATORY   (United States)

^b MASSEY UNIVERSITY   (New Zealand)

^c RIDDET INSTITUTE   (New Zealand)

^d MACDIARMID INSTITUTE FOR ADVANCED MATERIALS AND NANOTECHNOLOGY   (New Zealand)

^e NATIONAL CHUNG HSING UNIVERSITY   (Taiwan)

Author keywords

Enzyme mode of action; Nanostructure; Pectin; Pectin methylesterase; Rheology

Indexed keywords

CORRELATION METHODS; MERCURY COMPOUNDS; MOLECULES;

ABSOLUTE DEGREE OF BLOCKINESS; BLOCK SIZES; ENZYME MODE OF ACTION; FUNCTIONAL DOMAINS; HOMOGALACTURONAN; METHYLESTERIFICATION; MODE OF ACTION; PECTIN; PECTIN METHYLESTERASE; POLYGALACTURONASE;

ENZYMES;

EID: 84894071942     PISSN: 0268005X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.foodhyd.2014.01.022     Document Type: Article

References (49)

1. Artimo P., Jonnalagedda M., Arnold K., Baratin D., Csardi G., de Castro E., et al. ExPASy: SIB bioinformatics resource portal. Nucleic Acids Research 2012, 40(W1):W597-W603.
2. Atmodjo M.A., Hao Z., Mohnen D. Evolving views of pectin synthesis. Annual Review of Plant Biology 2013, 64(1):747-799.
3. Bordenave M. Analysis of pectin methyl esterases 1996, Springer-Verlag, New York.
4. Cameron R.G., Luzio G.A., Goodner K., Williams M.A.K. Demethylation of a model homogalacturonan with a salt-independent pectin methylesterase from citrus: I. Effect of pH on demethylated block size, block number and enzyme mode of action. Carbohydrate Polymers 2008, 71(2):287-299.
5. Cameron R.G., Luzio G.A., Vasu P., Savary B.J., Williams M.A.K. Enzymatic modification of a model homogalacturonan with the thermally tolerant pectin methylesterase from Citrus: 1. Nanostructural characterization, enzyme mode of action, and effect of pH. Journal of Agricultural and Food Chemistry 2011, 59(6):2717-2724.
6. Campa C., Holtan S., Nilsen N., Bjerkan T., Stokke B., SkjåK-BræK G. Biochemical analysis of the processive mechanism for epimerization of alginate by mannuronan C-5 epimerase AlgE4. Biochemical Journal 2004, 381:155-164.
7. Daas P.J., Meyer-Hansen K., Schols H.A., De Ruiter G.A., Voragen A.G.J. Investigation of the non-esterified galacturonic acid distribution in pectin with endopolygalacturonase. Carbohydrate Research 1999, 318(1-4):135-145.
8. Daas P.J., Voragen A.G., Schols H.A. Characterization of non-esterified galacturonic acid sequences in pectin with endopolygalacturonase. Carbohydrate Research 2000, 326(2):120-129.
9. Denes J.M., Baron A., Renard C.M., Pean C., Drilleau J.F. Different action patterns for apple pectin methylesterase at pH 7.0 and 4.5. Carbohydrate Research 2000, 327(4):385-393.
10. Ding J.L.C., Hsu J.S.F., Wang M.M.C., Tzen J.T.C. Purification and glycosylation analysis of an acidic pectin methylesterase in jelly fig (Ficusawkeotsang) achenes. Journal of Agricultural and Food Chemistry 2002, 50(10):2920-2925.
11. Ding J.L.C., Lee T.T.T., Wang M.M.C., Tai S.S.K., Tzen J.T.C. Cloning and expression of an acidic pectin methylesterase from jelly fig (Ficus awkeotsang). Journal of Agricultural and Food Chemistry 2000, 48(7):3052-3057.
12. Duvetter T., Fraeye I., Sila D.N., Verlent I., Smout C., Hendrickx M., et al. Mode of de-esterification of alkaline and acidic pectin methyl esterases at different pH conditions. Journal of Agricultural and Food Chemistry 2006, 54(20):7825-7831.
13. Duvetter T., Sila D.N., Van Buggenhout S., Jolie R., Van Loey A., Hendrickx M. Pectins in processed fruit and vegetables: part I-stability and catalytic activity of pectinases. Comprehensive Reviews in Food Science and Food Safety 2009, 8(2):75-85.
14. Fries M., Ihrig J., Brocklehurst K., Shevchik V.E., Pickersgill R.W. Molecular basis of the activity of the phytopathogen pectin methylesterase. EMBO Journal 2007, 26:3879-3887.
15. Giovane A., Laratta B., Loiudice R., Quagliuolo L., Castaldo D., Servillo L. Determination of residual pectin methylesterase activity in food products. Biotechnology and Applied Biochemistry 1996, 23:181-184.
16. Grasdalen H., Andersen A.K., Larsen B. NMR spectroscopy of the action pattern of tomato pectinesterase: generation of block structure in pectin by a multiple-attack mechanism. Carbohydrate Research 1996, 289:105-114.
17. Greenwood C.T., Milne E.A. Starch degrading and synthesizing enzymes: a discussion of their properties and action pattern. Advances in Carbohydrate Chemistry and Biochemistry 1968, 23:281-366.
18. Guillotin S.E., Bakx E.J., Boulenguer P., Mazoyer J., Schols H.A., Voragen A.G.J. Populations having different GalA blocks characteristics are present in commercial pectins which are chemically similar but have different functionalities. Carbohydrate Polymers 2005, 60(3):391-398.
19. Hotchkiss A.T., Savary B.J., Cameron R.G., Chau H.K., Brouillette J., Luzio G.A., et al. Enzymatic modification of pectin to increase its calcium sensitivity while preserving its molecular weight. Journal of Agricultural and Food Chemistry 2002, 50(10):2931-2937.
20. Hsiao E.S.L., Chen J.C.F., Tsai H.-Y., Khoo K.-H., Chen S.-T., Tzen J.T.C. Determination of N-glycosylation site and glycan structures of pectin methylesterase in jelly fig (Ficus awkeotsang) achenes. Journal of Agricultural and Food Chemistry 2009, 57:6757-6763.
21. Kashyap D.R., Vohra P.K., Chopra S., Tewari R. Applications of pectinases in the commercial sector: a review. Bioresource Technology 2001, 77(3):215-227.
22. Kim Y., Williams M.A.K., Galant A.L., Luzio G.A., Savary B.J., Vasu P., et al. Nanostructural modification of a model homogalacturonan with a novel pectin methylesterase: effects of pH on nanostructure, enzyme mode of action and substrate functionality. Food Hydrocolloids 2013, 33(1):132-141.
23. Kim Y., Teng Q., Wicker L. Action pattern of Valencia orange PME de-esterification of high methoxyl pectin and characterization of modified pectins. Carbohydrate Research 2005, 340(17):2620-2629.
24. Kim Y., Yoo Y.H., Kim K.O., Park J.B., Yoo S.H. Textural properties of gelling system of low-methoxy pectins produced by demethoxylating reaction of pectin methyl esterase. Journal of Food Science 2008, 73(5):C367-C372.
25. Komae K., Sone Y., Kakuta M., Misaki A. Purification and characterization of pectinesterase from Ficus awkeotsang. Agricultural and Biological Chemistry 1990, 54(6):1469-1476.
26. Lin T.-P., Liu C.-C., Chen S.-W., Wang W.-Y. Purification and characterization of pectinmethylesterase from Ficus awkeotsang Makino achenes. Plant Physiology 1989, 91(4):1445-1453.
27. Lofgren C., Guillotin S., Evenbratt H., Schols H., Hermansson A.M. Effects of calcium, pH, and blockiness on kinetic rheological behavior and microstructure of HM pectin gels. Biomacromolecules 2005, 6(2):646-652.
28. Luzio G.A., Cameron R.G. Demethylation of a model homogalacturonan with the salt-independent pectin methylesterase from citrus: II. Structure function analysis. Carbohydrate Polymers 2008, 71:300-309.
29. Luzio G.A., Cameron R. Contactless conductivity: an HPLC method to analyze degree of methylation of pectin. Proceedings of the Florida State Horticultural Society 2010, 123:213-216.
30. Mercadante D., Melton L.D., Jameson G.B., Williams M.A.K., Simone A.D. Substrate dynamics in enzyme action: rotations of monosaccharide subunits in the binding groove are essential for pectin methylesterase processivity. Biophysical Journal 2013, 104:1731-1739.
31. Micheli F., Sundberg B., Goldberg R., Richard L. Radial distribution pattern of pectin methylesterases across the cambial region of hybrid aspen at activity and dormancy. Plant Physiology 2000, 124(1):191-200.
32. 2+-pectin gels. Food Hydrocolloids 2012, 26(1):44-53.
33. Ngouémazong D.E., Tengweh F.F., Duvetter T., Fraeye I., Van Loey A., Moldenaers P., et al. Quantifying structural characteristics of partially de-esterified pectins. Food Hydrocolloids 2011, 25(3):434-443.
34. Peng C.C., Hsiao E.S.L., Ding J.L.C., Tzen J.T.C. Functional expression in Pichia pastoris of an acidic pectin methylesterase from jelly fig (Ficus awkeotsang). Journal of Agricultural and Food Chemistry 2005, 53(14):5612-5616.
35. Ralet M.C., Crepeau M.J., Lefebvre J., Mouille G., Hofte H., Thibault J.F. Reduced number of homogalacturonan domains in pectins of an Arabidopsis mutant enhances the flexibility of the polymer. Biomacromolecules 2008, 9(5):1454-1460.
36. Ralet M.C., Williams M.A.K., Tanhatan-Nasseri A., Ropartz D., Quéméner B., Bonnin E. Innovative enzymatic approach to resolve homogalacturonans based on their methylesterification pattern. Biomacromolecules 2012, 13(5):1615-1624.
37. Robyt J.F., French D. Multiple attack and polarity of action of porcine pancreatic α-amylase. Archives of Biochemistry and Biophysics 1970, 138:662-670.
38. Round A.N., Rigby N.M., MacDougall A.J., Morris V.J. Anew view of pectin structure revealed by acid hydrolysis and atomic force microscopy. Carbohydrate Research 2010, 345(4):487-497.
39. Savary B.J., Hotchkiss A.T., Fishman M.L., Cameron R.G., Shatters R.G. Development of a Valencia orange pectin methylesterase for generating novel pectin products. Advances in pectin and pectinase research 2003, 7. Kluwer Academic Press. H.A.S.A.G.J. Voragen, R. Visser (Eds.).
40. Ström A., Ribelles P., Lundin L., Norton I.T., Morris E.R., Williams M.A.K. Influence of pectin fine structure on the mechanical properties of calcium-pectin and acid-pectin gels. Biomacromolecules 2007, 8(9):2668-2674.
41. Tanhatan-Nasseri A., Crepeau M.J., Thibault J.F., Ralet M.C. Isolation and characterization of model homogalacturonans of tailored methylesterification patterns. Carbohydrate Polymers 2011, 86(3):1236-1243.
42. Thibault J.F., Renard C.M.G.C., Axelos M.A.V., Roger P., Crepeau M.J. Studies of the length of homogalacturonic regions in pectins by acid hydrolysis. Carbohydrate Research 1993, 238(4):271-286.
43. Van Buggenhout S., Messagie I., Maes V., Duvetter T., Van Loey A., Hendrickx M. Minimizing texture loss of frozen strawberries: effect of infusion with pectinmethylesterase and calcium combined with different freezing conditions and effect of subsequent storage/thawing conditions. European Food Research and Technology 2006, 223(3):395-404.
44. Widmer W. Analysis of biomass sugars and galacturonic acid by gradient anion exchange chromatography and pulsed amperometric detection without post-column addition. Biotechnology Letters 2011, 33:365-368.
45. Willats W.G.T., Knox J.P., Mikkelsen J.D. Pectin: new insights into anold polymer are starting to gel. Trends in Food Science & Technology 2006, 17(3):97-104.
46. Willats W.G., Orfila C., Limberg G., Buchholt H.C., van Alebeek G.J., Voragen A.G., et al. Modulation of the degree and pattern of methyl-esterification of pectic homogalacturonan in plant cell walls. Implications for pectin methyl esterase action, matrix properties, and cell adhesion. Journal of Biological Chemistry 2001, 276(22):19404-19413.
47. Winning H., Viereck N., Norgaard L., Larsen J., Engelsen S.B. Quantification of the degree of blockiness in pectins using 1H NMR spectroscopy and chemometrics. Food Hydrocolloids 2007, 21(2):256-266.
48. Winning H., Viereck N., Salomonsen T., Larsen J., Engelsen S.B. Quantification of blockiness in pectins - a comparative study using vibrational spectroscopy and chemometrics. Carbohydrate Research 2009, 344(14):1833-1841.
49. Yapo B.M., Lerouge P., Thibault J.-F., Ralet M.-C. Pectins from citrus peel cell walls contain homogalacturonans homogeneous with respect to molar mass, rhamnogalacturonan I and rhamnogalacturonan II. Carbohydrate Polymers 2007, 69:426-435.