Determination of N-glycosylation site and glycan structures of pectin methylesterase in jelly fig (Ficus awkeotsang) achenes

Journal of Agricultural and Food Chemistry

Volumn 57, Issue 15, 2009, Pages 6757-6763

  Hsiao, Eric S L ^a   Chen, Jeff C F ^a   Tsai, Hsien Yu ^b   Khoo, Kay Hooi ^b   Chen, Shui Tein ^b   Tzen, Jason T C ^a,c  

^a NATIONAL CHUNG HSING UNIVERSITY   (Taiwan)

^b INSTITUTE OF BIOLOGICAL CHEMISTRY   (Taiwan)

^c AGRICULTURAL BIOTECHNOLOGY RESEARCH CENTER   (Taiwan)

Author keywords

Glycan structure; Jelly fig; Mass analyses; N glycosylation; Pectin methylesterase

Indexed keywords

CARBOXYLESTERASE; PECTINESTERASE; POLYSACCHARIDE; VEGETABLE PROTEIN;

AMINO ACID SEQUENCE; ARTICLE; CHEMISTRY; ENZYMOLOGY; FICUS; GENETICS; GLYCOSYLATION; MASS SPECTROMETRY; METABOLISM; MOLECULAR GENETICS; SEQUENCE HOMOLOGY;

AMINO ACID SEQUENCE; CARBOXYLIC ESTER HYDROLASES; FICUS; GLYCOSYLATION; MOLECULAR SEQUENCE DATA; PLANT PROTEINS; POLYSACCHARIDES; SEQUENCE HOMOLOGY, AMINO ACID; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION;

ESCHERICHIA COLI; FICUS AWKEOTSANG;

EID: 68249098021     PISSN: 00218561     EISSN: None     Source Type: Journal    
DOI: 10.1021/jf9011278     Document Type: Article

References (22)

1. Gaffe, J.; Tiznado, M. E.; Handa. A. K. Characterization and functional expression of a ubiquitously expressed tomato pectin methylesterase. Plant Physiol. 1997, 114, 1547-1556.
2. Wen, F.; Zhu, Y.; Hawes, M. C. Effect of pectin methylesterase gene expression on pea root development. Plant Cell 1999, 11, 1129-1140.
3. Dorokhov, Y. L.; Makinen, K.; Frolova, O. Y.; Merits. A.; Saarinen, J.; Kalkkinen, N.; Atabekov, J. G.; Saarma, M. A novel function for a ubiquitous plant enzyme pectin methylesterase: the host-cell receptor for the tobacco mosaic virus movement protein. FEBS Lett. 1999, 461, 223-228.
4. Chen, M. H.; Sheng, J.; Hind, G.; Handa, A. K.; Citovsky, V. Interaction between the tobacco mosaic virus movement protein and host cell pectin methylesterases is required for viral cell-to-cell movement. EMBO J. 2000, 19, 913-920.
5. Gainvors, A.; Frezier, V.; Lemaresquier, H.; Lequart, C.; Aigle, M.; Belarbi, A. Detection of polygalacturonase, pectin-lyase and pectinesterase activities in a Saccharomyces eerevisiae strain. Yeast 1994, 10, 1311-1319.
6. Giovane, A.; Laratta, B.; Loiudice, R.; Quagliuolo, L.; Castaldo, D.; Servillo, L. Determination of residual pectin methylesterase activity in food products. Biotechnol. Appl. Biochem. 1996, 23, 181-184.
7. Lin, T. P.; Liu, C. C.; Chen, S. W.; Wang. W. Y. Purification and characterization of pectinmethylesterase from Ficus awkeotsang Makino achenes. Plant Physiol. 1989, 91, 1445-1453.
8. Huang, Y. C.; Chen, W. P.; Shao, Y. P. A study on the mechanism of gelatinization of awkeo-jelly. Chinu Hortic. 1980, 23, 117-126.
9. Ding, J. L. C.; Lee, T. T. T.; Wang, M. M. C.; Tai, S. S. K.; Tzen, J. T. C. Cloning and expression of an acidic pectin methylesterase from jelly fig (Ficus awkeotsang). J. Agric. Food Chem. 2000, 48, 3052-3057.
10. Lerouge, P.; Cabanes-Macheteau, M.; Rayon, C.; Fischette-Laine, A. C.; Gomord, V.; Faye, L. N-gylcoprotein biosynthesis in plant: Recent developments and future trends. Plant Mol. Biol. 1998, 38, 31-48.
11. Giovane. A.; Quagliuolo, L.; Castaldo, D.; Servillo. L.; Balestrieri, C. Pectin methyl esterase from Actinidia chinensis fruits, Phytochemistry 1990, 29, 2821-2823.
12. Rillo. L.; Castaldo, D.; Giovane, A.; Servillo, L.; Balestrieri, C.; Quagliuolo. L. Purification and properties of pectin methyleasterase from Mandarin orange fruit. J. Agric. Food Chem. 1992, 40, 591-593.
13. Randall, G. C.; Karel, G. Purification and characterization of a thermally tolerant pectin methylesterase from a commerical Valencia fresh frozen orange juice. J. Agric. Food Chem. 1996, 44, 458-462.
14. Ding, J. L. C.; Hsu, J. S. F.; Wang, M. M. C.; Tzen, J. T. C. Purification and glycosylation analysis of an acidic pectin methylesterase in jelly fig (Ficus awkeotsang) achenes. J. Agric. Food Chem. 2002, 50, 2920-2925.
15. Peng, C. C.; Hsiao, E. S.; Ding, J. L.; Tzen, J. T. C. Functional expression in Pichia pastoris of an acidic pectin methylesterase from jelly fig (Ficus awkeotsang). J. Agric. Food Chem. 2005, 53, 5612-5616.
16. Tzen, J. T. C.; Chuang, R. L. C.; Chen, J. C. F.; Wu, L. S. H. Coexistence of both oleosin isoforms on the surface of seed oil bodies and their individual stabilization to the organelles. J. Biochem. 1998, 123, 319-324.
17. Poison, A. Isolation of IgY from the yolks of eggs by a chloroform polyethylene glycol procedure, Immunol. Invest. 1990, 19, 253-258.
18. Carlsson, S. R. Isolation and characterization of glycoproteins. In Glycobiology, A practical approach; Fukuda, M., Kobata, A.. Eds.; Oxford University Press Inc.: New York, 1993; pp 1-26.
19. Ciucanu, L; Kerek, F. A simple and rapid method for the permethylation of carbohydrates. Carbohydr. Res. 1984, 131, 209-227.
20. Yu, S. Y.; Wu. S. W.; Khoo. K. H. Distinctive characteristics of MALDI-Q/TOF and TOF/TOF tandem mass spectrometry for sequencing of permethylated complex type N-glycans. Glvcoconiugate J. 2006, 25, 355-369.
21. Pelloux, J.; Rustérucci, C.; Mellerowicz, E. J. New insights into pectin methvlesterase structure and function. Trends Plum Sci. 2001, 12, 267-277.
22. Wildt, S.; Gerngross, T. U. The humanization of N-glycosylation pathways in yeast. Nat. Rev. Microbiol. 2005, 3, 119-128.