Enzymatic modification of pectin to increase its calcium sensitivity while preserving its molecular weight

Journal of Agricultural and Food Chemistry

Volumn 50, Issue 10, 2002, Pages 2931-2937

  Hotchkiss Jr , Arland T ^a   Savary, Brett J ^a   Cameron, Randall G ^b   Chau, Hoa K ^a   Brouillette, Janine ^a   Luzio, Gary A ^c,d   Fishman, Marshall L ^a  

^a EASTERN REGIONAL RESEARCH CENTER   (United States)

^b AGRICULTURAL RESEARCH SERVICE   (United States)

^c Hercules Incorporated   (United States)

^d Arbogast Pharmaceuticals   (United States)

Author keywords

Calcium sensitivity; HPSEC; Intrinsic viscosity; Molecular weight; NMR; Pectin deesterification; Pectin methylesterase

Indexed keywords

CALCIUM; GALACTURONIC ACID; ISOENZYME; MOVEMENT PROTEIN, PLANT VIRUS; PECTIN; PECTINESTERASE; VIRUS PROTEIN; PLANT VIRUS MOVEMENT PROTEIN;

ARTICLE; CHEMISTRY; CITRUS FRUIT; DEPOLYMERIZATION; ENZYME MECHANISM; ENZYME MODIFICATION; ENZYMOLOGY; ESTERIFICATION; GEL PERMEATION CHROMATOGRAPHY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; LIGHT SCATTERING; METABOLISM; MOLECULAR WEIGHT; NONHUMAN; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; ORANGE (FRUIT); PH; PROTON NUCLEAR MAGNETIC RESONANCE; REFRACTION INDEX; VISCOMETER; VISCOSITY;

CITRUS; VALENCIA;

CALCIUM; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CITRUS; ESTERIFICATION; HYDROGEN-ION CONCENTRATION; ISOENZYMES; MAGNETIC RESONANCE SPECTROSCOPY; MOLECULAR WEIGHT; PECTINS; PLANT VIRAL MOVEMENT PROTEINS; VIRAL PROTEINS; VISCOSITY;

EID: 0037041932     PISSN: 00218561     EISSN: None     Source Type: Journal    
DOI: 10.1021/jf011187w     Document Type: Article

References (44)

1. Industrial pectins: Sources, production and applications
2. Non-food uses of pectin
3. Contribution of pectins on health care
4. Low-methoxyl pectin prepared by pectinesterase from Aspergillus japonicus
5. Enzyme-demethylated pectinates and their gelation
6. Pectic enzymes
7. Pectins in mild alkaline conditions: β-elimination and kinetics of demethylation
8. Physicochemical properties of pectins in solution and gel states
9. Conformations and interactions of pectins. II. Influence of residue sequence on chain association in calcium pectate gels
10. Deesterification mode of pectin by pectin esterases of Aspergillus foetidus, tomatoes and alfalfa
11. Effect of molecular weight and method of deesterification on the gelling behavior of pectin
12. Distribution of free carboxyl groups in the pectin molecule after treatment with pectin esterase
13. Analysis of different deesterification mechanisms for pectin by enzymatic fingerprinting using endopectin lyase and endopolygalacturonase II from A. niger
14. Chromatographic study of highly methoxylated lime pectins deesterified by different pectin methyl-esterases
15. Process for selective extraction of pectins from plant material by differential pH
16. Modulation of the degree and pattern of methyl-esterification of pectic homogalacturonan in plant cell walls
17. Hydrocolloid stabilization of protein suspensions at low pH
18. Pectin methyl esterase from orange fruit: Characterization and localization by in-situ hybridization and immunohistochemistry
19. Kinetics of deesterification of pectin
20. Uber den mechanismus der enzymatischen verseifung von pektinstoffen
21. Mode of action of pectic enzymes. III. Site of initial action of tomato pectinesterase on highly esterified pectin
22. Analysis of pectin methyl esterases
23. NMR spectroscopy of the action pattern of tomato pectinesterase: Generation of block structure in pectin by a multiple-attack mechanism
24. Investigation of the action patterns of pectinmethylesterase isoforms through kinetic analyses and NMR spectroscopy
25. Different action patterns for apple pectin methylesterase at pH 7.0 and 4.5
26. Control of mung bean pectinmethylesterase isoform activities
27. Purification and some characteristics of two pectinesterase isozymes from orange
28. Perfusion chromatography separation of the tomato fruit-specific pectin methylesterase from a semipurified commercial enzyme preparation
29. Valencia orange peel pectin methylesterases and their use for generating novel pectin products
30. High-performance ion exchange chromatography of pectins
31. 1H NMR as a direct assay for pectinesterase activity
32. Compositional heterogeneity in pectic polysaccharides: NMR studies and statistical analysis
33. Multiple forms of pectinmethylesterase from citrus peel and their effects on juice cloud stability
34. Characterization of hemicellulose B from corn fiber by HPSEC with online molar mass and viscometric detection
35. Screening the physical properties of novel Pseudomonas exopolysaccharides by HPSEC with multi-angle light scattering and viscosity detection
36. Characterization of pectin flash-extracted from orange albedo by microwave heating under pressure
37. Progressive dissociation of pectin
38. The effect of the degree of esterification on the hydrodynamic properties of citrus pectin
39. Analysis of partially methyl-esterified galacturonic acid oligomers by high-performance anion-exchange chromatography and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
40. Investigation of the non-esterified galacturonic acid distribution in pectin.with endopolygalacturonase
41. Characterization of non-esterified galacturonic acid sequences in pectin with endopolygalacturonase
42. Quantification of the amount of galacturonic acid residues in block sequences in pectin homogalacturonan by enzymatic fingerprinting with exo- and endo-polygalacturonase II from Aspergillus niger