Identification and characterization of an unusual glycosyltransferase-like enzyme with β-galactosidase activity from a soil metagenomic library

Enzyme and Microbial Technology

Volumn 57, Issue , 2014, Pages 26-35

  Wang, Si di ^a   Guo, Geng shan ^a   Li, Liang ^a   Cao, Li chuang ^a   Tong, Ling ^a   Ren, Guang hui ^a   Liu, Yu huan ^a  

^a SUN YAT SEN UNIVERSITY   (China)

Author keywords

Galactosidase; Enzyme characterization; Glycosyltransferase; Metagenomic library; Sequence based classification

Indexed keywords

BIOCHEMICAL CHARACTERIZATION; ENZYME CHARACTERIZATION; ESCHERICHIA COLI BL21; GALACTO-OLIGOSACCHARIDES; GALACTOSIDASES; GLYCOSYL TRANSFERASE; GLYCOSYLTRANSFERASE ACTIVITY; METAGENOMIC LIBRARIES;

AMINO ACIDS; CHARACTERIZATION; ESCHERICHIA COLI; GENES; SUGARS;

ENZYMES;

AMINO ACID; BETA GALACTOSIDASE; GALACTOSE; GALACTOSE OLIGOSACCHARIDE; GENOMIC DNA; GLYCOSIDASE; GLYCOSYLTRANSFERASE; LACTOSE; RECOMBINANT ENZYME; BACTERIAL PROTEIN; OLIGOSACCHARIDE; RECOMBINANT PROTEIN;

AMINO ACID SEQUENCE; ARTICLE; BACTERIAL GENOME; CLONE; ENZYME ACTIVITY; ENZYME ANALYSIS; ESCHERICHIA COLI; GLYCOSYLATION; HYDROLYSIS; METAGENOMICS; MOLECULAR WEIGHT; NONHUMAN; NUCLEOTIDE SEQUENCE; REACTION TIME; RECOMBINANT PLASMID; RESTRICTION MAPPING; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS; SOIL; TEMPERATURE; THERMOSTABILITY; BACTERIAL GENE; BIOSYNTHESIS; CHINA; CLASSIFICATION; ENZYME SPECIFICITY; GENE LIBRARY; GENETICS; KINETICS; METABOLISM; METAGENOME; MICROBIOLOGY; MOLECULAR CLONING; MOLECULAR GENETICS; PHYLOGENY; SEQUENCE HOMOLOGY;

AMINO ACID SEQUENCE; BACTERIAL PROTEINS; BETA-GALACTOSIDASE; CHINA; CLONING, MOLECULAR; GENES, BACTERIAL; GENOMIC LIBRARY; GLYCOSYLTRANSFERASES; KINETICS; METAGENOME; MOLECULAR SEQUENCE DATA; OLIGOSACCHARIDES; PHYLOGENY; RECOMBINANT PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID; SOIL MICROBIOLOGY; SUBSTRATE SPECIFICITY;

EID: 84893682827     PISSN: 01410229     EISSN: 18790909     Source Type: Journal    
DOI: 10.1016/j.enzmictec.2014.01.007     Document Type: Article

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