Isolation and characterization of a new family 42 β-galactosidase from the thermoacidophilic bacterium Alicyclobacillus acidocaldarius: Identification of the active site residues

Biochimica et Biophysica Acta - Proteins and Proteomics

Volumn 1784, Issue 2, 2008, Pages 292-301

  Di Lauro, Barbara ^a   Strazzulli, Andrea ^a   Perugino, Giuseppe ^a   La Cara, Francesco ^a   Bedini, Emiliano ^b   Corsaro, Maria Michela ^b   Rossi, Mosè ^a,b   Moracci, Marco ^a  

^a INSTITUTE OF PROTEIN BIOCHEMISTRY   (Italy)

^b UNIVERSITY OF NAPLES FEDERICO II   (Italy)

Author keywords

Acid base catalyst; AraC XylS; Extremophile; Lactose hydrolysis; Lactulose; Reaction mechanism; Thermophilic galactosidase

Indexed keywords

BETA GALACTOSIDASE;

ALICYCLOBACILLUS ACIDOCALDARIUS; ARTICLE; BACTERIAL GENE; CATALYSIS; ENZYME ACTIVE SITE; ENZYME ANALYSIS; ENZYME ISOLATION; ENZYME MECHANISM; ENZYME PURIFICATION; ENZYME SPECIFICITY; GENE CONTROL; GENE EXPRESSION; GENETIC TRANSCRIPTION; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN FAMILY; SITE DIRECTED MUTAGENESIS; THERMOACIDOPHILIC BACTERIUM;

AMINO ACID SEQUENCE; ARAC TRANSCRIPTION FACTOR; BACTERIA; BETA-GALACTOSIDASE; BINDING SITES; CLONING, MOLECULAR; CONSERVED SEQUENCE; HYDROGEN-ION CONCENTRATION; KINETICS; MOLECULAR SEQUENCE DATA; MUTATION; SEQUENCE ALIGNMENT; TEMPERATURE; TRANSCRIPTION, GENETIC;

ALICYCLOBACILLUS ACIDOCALDARIUS; BACTERIA (MICROORGANISMS);

EID: 38549105991     PISSN: 15709639     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bbapap.2007.10.013     Document Type: Article

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