Microbial Transglutaminase: General Characteristics and Performance in Food Processing Technology

Food Biotechnology

Volumn 28, Issue 1, 2014, Pages 1-24

  de Góes Favoni, Silvana Pedroso ^a   Bueno, Fábio Rodrigo ^a  

^a Institute of Food Technology   (Brazil)

Author keywords

enzyme processing; food development; food proteins; microbial transglutaminase; technological properties

Indexed keywords

COMMERCIAL APPLICATIONS; FOOD PROCESSING TECHNOLOGY; FOOD PROTEINS; INTRAMOLECULAR CROSS-LINKS; MICROBIAL TRANSGLUTAMINASE; MICROBIAL TRANSGLUTAMINASE (MTGASE); TECHNOLOGICAL PROPERTIES; WATER HOLDING CAPACITY;

AMINO ACIDS; EMULSIFICATION; GELATION; PROTEINS; VEGETABLES;

ENZYMES;

EID: 84893370513     PISSN: 08905436     EISSN: 15324249     Source Type: Journal    
DOI: 10.1080/08905436.2013.870076     Document Type: Article

References (94)

1. ©2013Ajinomoto Foods Europe SAS. AvailableAccessed February 6, 2013 http:/transglutaminase.com
2. Ali, N.A., Ahmed, S.H., Mohamed, E.A., Ahmed, I.A.M. and Babiker, E.E. 2010. Effect of transglutaminase cross linking on the functional properties as a function of NaCl concentration of legumes protein isolate. Intern. J. Biol. Life Sci, 6 (1): 8 - 13.
3. Almeida, M.A. 2010. Transglutaminase e albumina de ovo em reestruturados cozidos congelados de frango, Piracicaba: Escola Superior de Agricultura Luiz de Queiroz.
4. ANBio2005Enzimas industriais e especiais. Documento elaborado no âmbito do convênio 143/2005 CGEE - IQ/UFRJ-FUJB, relativo ao Termo de Referência Fórum de Biotecnologia 03.2005. AvailableAccessed November 10, 2012 http://www.anbio.org.br/pdf/2/tr03_enzimas.pdf
5. Ando, H., Adachi, M., Umeda, K., Matsuura, A., Nonaka, M., Uchio, R., Tanaka, H. and Motoki, M. 1989. Purification and characteristics of a novel transglutaminase derived from microorganisms. Agric. Biol. Chem, 53: 2613 - 2617.
6. Ávila, M.D.R., Ordóñez, J.A., De La Hoz, L., Herrero, A.M. and Cambero, M.I. 2010. Microbial transglutaminase for cold-set binding of unsalted/salted pork models and restructured dry ham. Meat Sci, 84: 747 - 754.
7. Babiker, E.F.E. 2000. Effect of transglutaminase treatment on the functional properties of native and chymotrypsin-digested soy protein. Food Chem, 70: 139 - 145.
8. Bagagli, M.P. 2009. Produção de transglutaminase de Streptomyces sp. P20, caracterização e aplicação da enzima bruta, Campinas: Universidade Estadual de Campinas.
9. Barreiro, F.J. and Seselovsky, R. 2003. Usos de La transglutaminasa em la indústria alimentaria. Elaboración de carne reconstituída. Rev. Invenio, 6 (10): 157 - 164.
10. Benjakul, S., Visessangua, W., Phatchrat, S. and Tanaka, M. 2003. Chitosan affects transglutaminase-induced Surimi gelation. J. Food Bioch, 27: 53 - 66.
11. Bernard, B.K., Tsubuku, S. and Shioya, S. 1998. Acute toxicity and genotoxicity studies of a microbial transglutaminase. Int. J. Toxicol, 17: 703 - 721.
12. Bönish, M.P., Lauber, S. and Kulozik, U. 2004. Effect of ultra high temperature treatment on the enzymatic cross-linking of micellar casein and sodium caseinate by transglutaminase. J. Food Sci, 69: 398 - 404.
13. Bönish, M.P., Tolkach, A. and Kulozik, U. 2006. Inactivation of an indigenous transglutaminase inhibitor in milk serum by means of UHT-treatment and membrane separation techniques. Int. Dairy J, 16: 669 - 678.
14. Bönish, M.P., Huss, M., Lauber, S. and Kulozik, U. 2007a. Yoghurt gel formation by means of enzymatic protein cross-linking during microbial fermentation. Food Hydrocolloids, 21 (4): 585 - 595.
15. Bönish, M.P., Huss, M., Weitl, K. and Kulozik, U. 2007b. Transglutaminase cross-linking of milk proteins and impact on yoghurt gel properties. Int. Dairy J, 17 (11): 1360 - 1371.
16. BRASIL. 2003. Agência Nacional de Vigilância Sanitária. Resolução RDC n° 348, de 02 de dezembro de 2003. Aprova de forma complementar ao Anexo da Resolução CNNPA n° 24 de 1976, a utilização de enzimas na indústria de alimentos, Diário Oficial, Brasília: 03 de dezembro de 2003.
17. BRASIL. 2005. Agência Nacional de Vigilância Sanitária. Resolução RDC n° 278, de 22 de setembro de 2005. Aprova as categorias de alimentos e embalgens dispensados e com obrigatoriedade de registro, Diário Oficial, Brasília: 22 de setembro 2005.
18. Carlos, H.P. and Carreño, F.G. 2007. Transglutaminasas: de Importante Papel Fisiológico enlos Seres Vivos al Desarrollo Novedoso de Tecnología de Alimentos. Rev. Soc. Mex. Biotecnol. Bioeng, 11 (1): 21 - 27.
19. Celis, F.T. 2009. Efeito da reticulação induzida pela transglutaminase e o glutaraldeido sobre as propriedades das microparticulas obtidas por coacervação complexa, Campinas: Universidade Estadual de Campinas.
20. Clarke, D.D., Neidle, A., Sarkar, N.K. and Waelsch, H. 1959a. Metabolic activity of protein amide groups. Arch. Biochem. Bioph, 79: 277 - 279.
21. Clarke, D.D., Mycek, M.J., Neidle, A. and Waelsch, H. 1959b. The incorporation of amines into protein. Arch. Biochem. Bioph, 79: 338 - 354.
22. Cozzolino, A., Di Pierro, P., Mariniello, L., Sorrentino, A., Masi, P. and Porta, R. 2003. Incorporation of whey proteins into cheese curd by using transglutaminase. Biotechnol. Appl. Biochem, 38: 289 - 295.
23. Date, M., Yokoyama, K., Umezaway, Y., Matsui, H. and Kikuchi, Y. 2003. Production of native-type Streptoverticilium mobaraense transglutaminase in. Corynebacterium glutamicum. Appl. Environ. Microbiol, 69 (5): 3011 - 3014.
24. Duran, R., Jungua, M., Schmitter, J.M., Gancet, C. and Gaulas, P. 1998. Purification, characterization, and gene cloning to transglutamianse from Streptoverticillium cinnamoneum CBS 683.68. Biochimie, 80: 313 - 319.
25. De Jong, G.A.H., Boumans, J.W.L. and Wijngaards, G. 2009. Food grade transglutaminase inhibitor and uses thereof. U.S. Patent No. 7491410, 2009,
26. De Jong, G.A.H. and Koppelman, S.J. 2002. Transglutaminase catalyzed reactions: Impact on food applications. J. Food Sci, 67 (8): 2798 - 2806.
27. Faria, D.S. 2010. Estudo dos efeitos da aplicação de transglutaminase em bebida láctea fermentada com alto conteúdo de soro, São Caetano do Sul: Instituto Mauá de Tecnologia.
28. Ferreira, M.S., Mársico, E.T., Medeiros, R.J., Pombo, C.R., Freitas, M.Q., Clemente, S.C.S. and Conte Junior, C.A. 2012. Comparação das características físico-químicas e sensoriais de hambúrgueres de carne bovina elaborados com cloreto de sódio, polifosfato e transglutaminase. Rev. Bras. Med. Vet, 34 (1): 52 - 60.
29. Folk, J.E. and Cole, P.W. 1966. Transglutaminase: mechanistic features of the active site as determined by kinetic and inhibitor studies. Biochim. Biophys. Acta, 122 (2): 244 - 264.
30. Folk, J.E. and Finlayson, J.S. 1977. The ε{lunate}-(γ-glutamyl)lysine cross-link and the catalytic role of transglutaminase. Adv. Protein Chem, 31: 1 - 133.
31. Gauche, C., Tomazi, T. and Bordignon-Luiz, M.T. 2008. Polimerização de proteínas do leite por transglutaminase: modificação das propriedades funcionais e aplicação em produtos lácteos. Bol. Centro Pesqui. Process. Aliment, 26 (1): 111 - 122.
32. Gauche, C., Vieira, J.T.C., Barreto, P.L.M. and Bordignon-Luiz, M.T. 2009. Polimerização de proteínas do soro de leite com transglutaminase - efeito do tratamento térmico no comportamento reológico. Paper presented at the 10th Congresso Brasileiro de Polímeros, Outubro de 2009, Foz do Iguaçu.
33. Gerrard, J.A., Fayle, S.E., Wilson, A.J., Newberry, M.P., Rossa, M. and Kavale, S. 1998. Dough properties and crumb strength of white pan bread as affected by microbial transglutaminase. J. Food Sci, 63 (3): 472 - 475.
34. Gerrard, J.A., Newberry, M.P., Ross, M., Wilson, A.J., Fayle, S.E. and Kavale, S. 2000. Pastry lift and croissant volume as affected by microbial transglutaminase. J. Food Sci, 65 (2): 312 - 314.
35. Gerrard, J.A. and Sutton, K.H. 2005. Addition of transglutaminase to cereal products may generate the epitope responsible for coeliac disease. Trends Food Sci. Tech, 16: 510 - 512.
36. Góes-Favoni, S.P., Bueno, F.R., Marcussi, F. and Pagani, C.P. 2012. Características físicas e viabilidade celular de bebida fermentada de soja contendo transglutaminase microbiana, 21 - 23. Paper presented at the Fatecal VI Simpósio de Tecnologia em Alimentos, Marília, November.
37. Gragnani, M.A.L. 2010. Produção e avaliação de pão de forma com Triticale e enzima transglutaminase microbiana, Campinas: Universidade Estadual de Campinas.
38. Gujral, H.S. and Rosell, C.M. 2004. Functionality of rice flour modified with a microbial transglutaminase. J. Cereal Sci, 39: 225 - 230.
39. Ho, M.L., Leu, S.Z., Hsieh, J.F. and Jiang, S.T. 2000. Technical approach to simplify the purification method and characterization of microbial transglutaminase produced from. Streptoverticillium ladakanum. J. Food Sci, 65 (1): 76 - 80.
40. Hinz, K., Huppertz, T., Kulozik, U. and Kelly, A.L. 2007. Influence of enzymatic cross-linking on milk fat globules and emulsifying properties of milk proteins. Int. Dairy J, 17: 289 - 293.
41. Hinz, K., Huppertz, T. and Kelly, A.L. 2012. Susceptibility of the individual caseins in reconstituted skim milk to cross-linking by transglutaminase: influence of temperature, pH and mineral equilibria. J. Dairy Res, 79 (4): 414 - 421.
42. Icekson, I. and Apelbaum, A. 1987. Evidence for transglutaminase activity in plant tissue. Plant Physiol, 84: 972 - 974.
43. Ikura, K., Kometani, K., Yoshikawa, M., Sasaki, R. and Chiba, H. 1980. Cross-linking of casein components by transglutaminase. Agric. Biol. Chem, 44: 1567 - 1573.
44. Jaros, D., Partschefeld, C., Henle, T. and Rohm. H. (2006). Transglutaminase in dairy products: chemistry, physics, applications. J. Texture Stud, 37 (2) 113 - 155.
45. Jaros, D., Heidig, C. and Harald, R. 2007. Enzymatic modification through microbial transglutaminase enhances the viscosity of stirred yogurt. J. Texture Stud, 38 (2): 179 - 198.
46. Kanaji, T., Ozaki, H., Takao, T., Kawajiri, H., Ide, H., Motoki, M. and Shimonishi, Y. 1993. Primary structure of microbial transglutaminase from Streptoverticillium sp. strain s-8112. J. Biol. Chem, 268 (16): 11565 - 11572.
47. Kang, H. and Cho, Y.D. 1996. Purification and properties of transglutaminase from soybean (Glycine max) leaves. Biochem. Bioph. Res. Comm, 223 (2): 288 - 292.
48. Kang, I.J., Matsumura, Y., Ikura, K., Motoki, M., Sakamoto, H. and Mori, T. 1994. Gelation and properties of soybean glycinine in a transglutaminase-catalyszed system. J. Agric. Food Chem, 42 (1): 159 - 165.
49. Kashiwagi, T., Yokoyama, K.-I., Ishikawa, K., Ono, K., Ejima, D., Matsui, H. and Suzuki, E.-I. 2002. Crystal structure of microbial transglutaminase from. streptoverticillium mobaraense. J. Biol. Chem, 277 (46): 44252 - 44260.
50. Katayama, K., Chin, K.B., Yoshihara, S. and Muguruma, M. 2006. Microbial transglutaminase improves the property of meat protein and sausage texture manufactured with low-quality pork loins. Aust. J. Anim. Sci, 19 (1): 102 - 108.
51. Kato, A., Wada, T., Kobayashi, K., Seguro, K. and Motoki, M. 1991. Ovomucin-food protein conjugates prepared through the transglutaminase reaction. Agric. Biol. Chem, 55 (4): 1027 - 1031.
52. Klein, J., Guzman, E. and Kuehn, G. 1992. Purification and partial characterization of transglutaminase from. Physarum polycephalum. J. Bacteriol, 174 (8): 2599 - 2605.
53. Kuraishi, C., Yamazaki, K. and Susa, Y. 2001. Transglutaminase: Its utilization in the food industry. Food Rev. Int, 17 (2): 221 - 246.
54. Kuraishi, C., Sakamoto, J., Yamazaki, K., Susa, Y., Kuhara, C. and Soeda, T. 1997. Production of restructured meat using microbial transglutaminase without salt or cooking. J. Food Sci, 62 (3): 488 - 490.
55. Kurth, L. and Rogers, P.J. 1984. Transglutaminase catalyzed cross-linking of myosin to soy protein, casein and gluten. J Food Sci, 49 (2): 573 - 576.
56. Kütemeyer, C., Froeck, M., Werlein, H.D. and Watkinson, B.M. 2005. The influence of salts and temperature on enzymatic activity of microbial transglutaminase. Food Control, 16 (8): 735 - 737.
57. Lauber, S., Henle, T. and Klostermeyer, H. 2000. Relationship between the cross-linking of caseins by transglutaminase and the gel strength of yogurt. Eur. Food Res. Tech, 210: 305 - 309.
58. Lorenzen, P.C. 2007. Effects of varying time/temperature-conditions of pre-heating and enzymatic cross-linking on techno-functional properties of reconstituted dairy ingredients. Appl. Microbiol. Biotechnol, 40 (6): 700 - 708.
59. Lorenzen, P.C., Neve, H., Mautner, A. and Schlimme, E. 2002. Effect of enzymatic cross-linking of milk proteins on functional properties of set-style yoghurt. Int. J. Dairy Tech, 55 (3): 152 - 157.
60. Macedo, J.A. 2009. Produção, purificação, caracterização e aplicação de transglutaminase de Streptomyces sp. CBMAI 837, Campinas: Universidade Estadual de Campinas.
61. Macedo, J. and Sato, H. 2005. Propriedades e aplicações da transglutaminase microbiana em alimentos. Alim. Nutr, 16 (4): 413 - 419.
62. Marchi, J.F. 1997. Desenvolvimento e avaliação de produtos à base de polpa e surimi produzidos a partir de tilápia Nilótica, Oreochromis niloticus L. Viçosa: Universidade Federal de Viçosa.
63. Margosiak, S.A., Dharma, A., Bruce-Carver, M.R., Gonzales, A.P., Louie, D. and Kuehn, G.D. 1990. Identification of the large subunit of ribulose 1,5-bisphosphate carboxylase/oxygenase as a substrate for transglutaminase in Medicago sativa L. (Alfalfa). Plant Physiol, 92: 88 - 96.
64. Mehta, K. and Eckert, R. 2005. Transglutaminases: Family of Enzymes with Diverse Functions, Basel, Switzerland: Karger Press.
65. Miwa, N., Kawasaki-Shi, K., Kumazawa, Y., Nakagoshi, H. and Sakaguchi, S. 2007. Method for modifying raw material milk and dairy product prepared by using the modified raw material milk, CA: Ajinomoto Co. Inc., Patent number. 2 358 381 C, 2007
66. Motoki, M. and Kumazawa, Y. 2000. Recent research trends in transglutaminase technology for food processing. Food Sci. Technol. Res, 6 (3): 151 - 160.
67. Motoki, M. and Seguro, K. 1998. Transglutaminase and its use for food processing. Trends Food Sci. Tech, 5 (9): 204 - 210.
68. Nonaka, M., Sakamoto, H., Toiguchi, S., Kawajiri, H., Soeda, T. and Motoki, M. 1992. Sodium caseinate and skim milk gels formed by incubation with microbial. J. Food Sci, 57 (5): 1214 - 1241.
69. 2+-independent microbial transglutaminase. Biosci. Biotechnol. Biochem, 60: 131 - 133.
70. Ozer, B., Kirmaci, H.A., Oztekin, S., Hayaloglu, A. and Atamer, M. 2007. Incorporation of microbial transglutaminase into nonfat yogurt production. Int. Dairy J, 17 (3): 199 - 207.
71. Özrenk, E. 2006. The use of transglutaminase in dairy products. Int. J. Dairy Technol, 59 (1): 1 - 7.
72. Pedersen, M.H., Hansen, T.K., Sten, E., Seguro, K., Ohtsuka, T., Morita, A., Bindslev-Jensen, C. and Poulsen, L.K. 2004. Evaluation of the potential allergenicity of the enzyme microbial transglutaminase using the 2001 FAO/WHO Decision Tree. Mol. Nutr. Food Res, 48: 434 - 440.
73. Pereira, A.J. 2003. Desenvolvimento de tecnologia para produção e utilização da polpa de carne de carpa prateada (Hypophthalmichthys molitrix) na elaboração de produtos reestruturados, fishburger'e nugget. Curitiba: Universidade Federal do Paraná.
74. Piccolo, K.C. 2006. Avaliação do efeito da enzima transglutaminase no processo de produção de requeijão cremoso, São Caetano do Sul: Instituto Mauá de Tecnologia.
75. Renzetti, S., Bello, F.D. and Arentdt, E.K. 2008. Microstruture, fundamental rheology and baking characteristics of batters and breads from different gluten-free flours treated with a microbial transglutaminase. J. Cereal Sci, 48 (1): 33 - 45.
76. Rossa, P.N., Sá, E.M.F., Burin, V.M. and Bordignon-Luiz, M.T. 2011. Optimization of microbial transglutaminase activity in ice cream using response surface methodology. LWT - Food Sci. Tech, 44: 29 - 34.
77. Sakamoto, H., Kumazawa, Y. and Motoki, M. 1994. Strength of protein gels prepared with microbial transglutaminase as related to reaction conditions. J. Food Sci, 59 (4): 866 - 871.
78. Seguro, K., Kumazawa, Y., Kuraishi, C., Sakamoto, H. and Motoki, M. 1996. The epsilon-(gamma-glutamyl) lysine moiety in cross-linked casein is an available source of lysine for rats. J. Nutr, 126 (10): 2557 - 2562.
79. Schäfer, C., Neidhart, S. and Carle, R. 2011. Application and sensory evaluation of enzymatically texturized vegetable proteins in food models. Eur. Food Res. Technol, 232: 1043 - 1056.
80. Soares, L.H.B., Assmann, F. and Ayub, M.A.Z. 2003. Purification and properties of a transglutaminase produced by a Bacillus circulans strain isolated from the Amazon environmente. Biotechnol. Appl. Biochem, 37 (3): 295 - 299.
81. Soares, L.H.B., Albuquerque, P.M., Assmann, F. and Ayub, M.A.Z. 2004. Physicochemical properties of three food proteins treated with transglutaminase. Ciênc. Rural, 34 (4): 1219 - 1223.
82. Souza, C.F.V. 2008. Produção em cultivo submerso e no estado sólido e caracterização da transglutaminase (EC 2.3.2.13) no isolado amazônico, Bacillus circulans. Porto Alegre: Universidade Federal do Rio Grande do Sul.
83. Storck, C.R., Pereira, J.M., Pereira, G.W., Rodrigues, A.O., Gularte, M.A. and Dias, A.R.G. 2009. Características tecnológicas de pães elaborados com farinha de arroz e transglutaminase. Braz. J. Food Technol. II SSA,
84. Taguchi, S., Arakawa, K., Yokoyama, K., Takehana, S., Takagi, H. and Momose, H. 2002. Overexpression and purification of microbial pro-transglutaminase from Streptomyces cinnamoneum and in vitro processing by Streptomyces albogriseolus proteases. J. Biosci. Bioeng, 94 (5): 478 - 481.
85. Tang, C-H., Wu, H., Yu, H-P., Li, L., Chen, Z. and Yang, X-Q. 2005. Coagulation and gelation of soy protein isolates induced by microbial transglutaminase. J. Food Biochem, 30: 35 - 55.
86. Tang, C-H., Li, L. and Yang, X-Q. 2006. Influence of transglutaminase-induced cross-linking on in vitro digestibility of soy protein isolate. J. Food Biochem, 30 (6): 718 - 731.
87. Tang, C-H. 2007. Effect of thermal pretreatment of raw soymilk on the gel strength and microstructure of tofu induced by microbial transglutaminase. Food Sci. Technol, 40 (8): 1403 - 1409.
88. Truong, V-D., Clare, D.A., Catignani, G.L. and Swaisgood, H.E. 2004. Cross-linking and rheological changes of whey proteins treated with microbial transglutaminase. J. Agric. Food Chem, 52 (5): 1170 - 1176.
89. Vácha, F., Novik, I., Špička, J. and Podola, M. 2006. Determination of the effect of microbial transglutaminase on technological properties of common carp (Cyprinus carpio L.) meat. Czech J. Anim. Sci, 51 (12): 535 - 542.
90. Yasir, S.B., Sutton, K.H., Newberry, M.P., Andrews, N.R. and Gerrard, J.A. 2007. The impact of transglutaminase on soy proteins and tofu texture. Food Chem, 104 (4): 1491 - 1501.
91. Yokoyama, K., Nio, N. and Kikuchi, Y. 2004. Properties and applications of microbial transglutaminase. Appl. Microbiol. Biotechnol, 64 (4): 447 - 454.
92. Yoshida, C.M.P. and Antunes, A.J. 2009. Aplicação de filmes proteicos à base de soro de leite. Ciênc. Tecnol. Alim, 29 (2): 420 - 430.
93. Zhu, Y., Rinzema, A., Tramper, J. and Bol, J. 1995. Microbial transglutaminase - a review of its production and application in food processing. Appl. Microbiol. Biotechnol, 44 (3-4): 277 - 282.
94. Zhu, Y., Rinzema, A., Tramper, J., Bruin, E. and Bol, J. 1998. Fed-batch fermentation dealing with nitrogen limitation in microbial transglutaminase production by Streptoverticillium mobaraense. Appl. Microbiol. Biotechnol, 49: 251 - 257.