Precision-cut liver slices from diet-induced obese rats exposed to ethanol are susceptible to oxidative stress and increased fatty acid synthesis

American Journal of Physiology - Gastrointestinal and Liver Physiology

Volumn 306, Issue 3, 2014, Pages

  Duryee, Michael J ^a,b,d   Willis, Monte S ^c   Schaffert, Courtney S ^a,b   Reidelberger, Roger D ^e   Dusad, Anand ^a,b   Anderson, Daniel R ^c   Klassen, Lynell W ^a,b   Thiele, Geoffrey M ^a,b,d  

^a VA NEBRASKA WESTERN IOWA HEALTH CARE SYSTEM   (United States)

^b UNIVERSITY OF NEBRASKA MEDICAL CENTER   (United States)

^c UNIVERSITY OF NORTH CAROLINA SCHOOL OF MEDICINE   (United States)

^d UNIVERSITY OF NEBRASKA MEDICAL CENTER   (United States)

^e VA NWIHCS   (United States)

Author keywords

Alcohol liver disease; Fatty liver disease; Obesity; Oxidative stress; Precision cut liver slices

Indexed keywords

ALCOHOL; GLUTATHIONE; HEME OXYGENASE 1; INTERLEUKIN 6; SMOOTH MUSCLE ACTIN; TRIACYLGLYCEROL; TUMOR NECROSIS FACTOR ALPHA;

ALCOHOL METABOLISM; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; CYTOKINE PRODUCTION; DIET; FATTY ACID SYNTHESIS; FATTY LIVER; LIPID PEROXIDATION; LIVER SLICE; MALE; NONHUMAN; OXIDATIVE STRESS; PRECISION CUT LIVER SLICE; PRIORITY JOURNAL; RAT;

ALCOHOL LIVER DISEASE; FATTY LIVER DISEASE; OBESITY; OXIDATIVE STRESS; PRECISION-CUT LIVER SLICES;

ANIMALS; DIET, HIGH-FAT; ETHANOL; FATTY ACIDS; FATTY LIVER; INTERLEUKIN-6; LIPID PEROXIDATION; LIVER; MALE; MODELS, ANIMAL; OBESITY; OXIDATIVE STRESS; RATS; RATS, SPRAGUE-DAWLEY; TUMOR NECROSIS FACTOR-ALPHA;

EID: 84893350025     PISSN: 01931857     EISSN: 15221547     Source Type: Journal    
DOI: 10.1152/ajpgi.00124.2013     Document Type: Article

References (47)

1. Arteel GE. Animal models of alcoholic liver disease. Dig Dis 28: 729-736, 2010.
2. Berk PD, Zhou S, Bradbury MW. Increased hepatocellular uptake of long chain fatty acids occurs by different mechanisms in fatty livers due to obesity or excess ethanol use, contributing to development of steatohepatitis in both settings. Trans Am Clin Climatol Assoc 116: 335-344; discussion 345, 2005.
3. Braunersreuther V, Viviani GL, Mach F, Montecucco F. Role of cytokines and chemokines in non-alcoholic fatty liver disease. World J Gastroenterol 18: 727-735, 2012.
4. Brunt EM. Nonalcoholic steatohepatitis: definition and pathology. Semin Liver Dis 21: 3-16, 2001.
5. Buettner R, Parhofer KG, Woenckhaus M, Wrede CE, Kunz-Schughart LA, Scholmerich J, Bollheimer LC. Defining high-fat-diet rat models: metabolic and molecular effects of different fat types. J Mol Endocrinol 36: 485-501, 2006.
6. Cani PD, Amar J, Iglesias MA, Poggi M, Knauf C, Bastelica D, Neyrinck AM, Fava F, Tuohy KM, Chabo C, Waget A, Delmee E, Cousin B, Sulpice T, Chamontin B, Ferrieres J, Tanti JF, Gibson GR, Casteilla L, Delzenne NM, Alessi MC, Burcelin R. Metabolic endotoxemia initiates obesity and insulin resistance. Diabetes 56: 1761-1772, 2007.
7. Cani PD, Possemiers S, Van de Wiele T, Guiot Y, Everard A, Rottier O, Geurts L, Naslain D, Neyrinck A, Lambert DM, Muccioli GG, Delzenne NM. Changes in gut microbiota control inflammation in obese mice through a mechanism involving GLP-2-driven improvement of gut permeability. Gut 58: 1091-1103, 2009.
8. Carmiel-Haggai M, Cederbaum AI, Nieto N. Binge ethanol exposure increases liver injury in obese rats. Gastroenterology 125: 1818-1833, 2003.
9. Carmiel-Haggai M, Cederbaum AI, Nieto N. A high-fat diet leads to the progression of non-alcoholic fatty liver disease in obese rats. FASEB J 19: 136-138, 2005.
10. Chavez-Tapia NC, Rosso N, Tiribelli C. Effect of intracellular lipid accumulation in a new model of non-alcoholic fatty liver disease. BMC Gastroenterol 12: 20, 2012.
11. Clouston AD, Powell EE. Interaction of non-alcoholic fatty liver disease with other liver diseases. Best Pract Res Clin Gastroenterol 16: 767-781, 2002.
12. Compare D, Coccoli P, Rocco A, Nardone OM, De Maria S, Carteni M, Nardone G. Gut-liver axis: the impact of gut microbiota on non alcoholic fatty liver disease. Nutr Metab Cardiovasc Dis 22: 471-476, 2012.
13. Crow KE, Cornell NW, Veech RL. The rate of ethanol metabolism in isolated rat hepatocytes. Alcohol Clin Exp Res 1: 43-50, 1977.
14. Das SK, Balakrishnan V. Role of cytokines in the pathogenesis of non-alcoholic fatty liver disease. Indian J Clin Biochem 26: 202-209, 2011.
15. Day CP, James OF. Steatohepatitis: a tale of two "hits"? Gastroenterology 114: 842-845, 1998.
16. de La Serre CB, Ellis CL, Lee J, Hartman AL, Rutledge JC, Raybould HE. Propensity to high-fat diet-induced obesity in rats is associated with changes in the gut microbiota and gut inflammation. Am J Physiol Gastrointest Liver Physiol 299: G440-G448, 2010.
17. Dhibi M, Brahmi F, Mnari A, Houas Z, Chargui I, Bchir L, Gazzah N, Alsaif MA, Hammami M. The intake of high fat diet with different trans fatty acid levels differentially induces oxidative stress and non alcoholic fatty liver disease (NAFLD) in rats. Nutr Metab (Lond) 8: 65, 2011.
18. Eriksson CJ, Sippel HW, Forsander OA. The determination of acetaldehyde in biological samples by head-space gas chromatography. Anal Biochem 80: 116-124, 1977.
19. Gabele E, Dostert K, Dorn C, Patsenker E, Stickel F, Hellerbrand C. A new model of interactive effects of alcohol and high-fat diet on hepatic fibrosis. Alcohol Clin Exp Res 35: 1361-1367, 2011.
20. Gao B. Hepatoprotective and anti-inflammatory cytokines in alcoholic liver disease. J Gastroenterol Hepatol 27, Suppl 2: 89-93, 2012.
21. Garcia MC, Amankwa-Sakyi M, Flynn TJ. Cellular glutathione in fatty liver in vitro models. Toxicol In Vitro 25: 1501-1506, 2011.
22. Gong P, Cederbaum AI. Nrf2 is increased by CYP2E1 in rodent liver and HepG2 cells and protects against oxidative stress caused by CYP2E1. Hepatology 43: 144-153, 2006.
23. Gyamfi D, Everitt HE, Tewfik I, Clemens DL, Patel VB. Hepatic mitochondrial dysfunction induced by fatty acids and ethanol. Free Radic Biol Med 53: 2131-2145, 2012.
24. Han P, Zhang YY, Lu Y, He B, Zhang W, Xia F. Effects of different free fatty acids on insulin resistance in rats. Hepatobiliary Pancreat Dis Int 7: 91-96, 2008.
25. Jin X, Zimmers TA, Perez EA, Pierce RH, Zhang Z, Koniaris LG. Paradoxical effects of short-and long-term interleukin-6 exposure on liver injury and repair. Hepatology 43: 474-484, 2006.
26. Keshavarzian A, Holmes EW, Patel M, Iber F, Fields JZ, Pethkar S. Leaky gut in alcoholic cirrhosis: a possible mechanism for alcoholinduced liver damage. Am J Gastroenterol 94: 200-207, 1999.
27. Kim M, Yang SG, Kim JM, Lee JW, Kim YS, Lee JI. Silymarin suppresses hepatic stellate cell activation in a dietary rat model of non-alcoholic steatohepatitis: analysis of isolated hepatic stellate cells. Int J Mol Med 30: 473-479, 2012.
28. Klassen LW, Thiele GM, Duryee MJ, Schaffert CS, DeVeney AL, Hunter CD, Olinga P, Tuma DJ. An in vitro method of alcoholic liver injury using precision-cut liver slices from rats. Biochem Pharmacol 76: 426-436, 2008.
29. Krajmalnik-Brown R, Ilhan ZE, Kang DW, DiBaise JK. Effects of gut microbes on nutrient absorption and energy regulation. Nutr Clin Pract 27: 201-214, 2012.
30. Larter CZ, Yeh MM. Animal models of NASH: getting both pathology and metabolic context right. J Gastroenterol Hepatol 23: 1635-1648, 2008.
31. Leung TM, Nieto N. CYP2E1 and oxidant stress in alcoholic and non-alcoholic fatty liver disease. J Hepatol 58: 395-398, 2013.
32. Liangpunsakul S, Chalasani N. What should we recommend to our patients with NAFLD regarding alcohol use? Am J Gastroenterol 107: 976-978, 2012.
33. Morton GJ, Thatcher BS, Reidelberger RD, Ogimoto K, Wolden-Hanson T, Baskin DG, Schwartz MW, Blevins JE. Peripheral oxytocin suppresses food intake and causes weight loss in diet-induced obese rats. Am J Physiol Endocrinol Metab 302: E134-E144, 2012.
34. Nakamura MT, Cho HP, Clarke SD. Regulation of hepatic delta-6 desaturase expression and its role in the polyunsaturated fatty acid inhibition of fatty acid synthase gene expression in mice. J Nutr 130: 1561-1565, 2000.
35. Niemela O, Parkkila S, Juvonen RO, Viitala K, Gelboin HV, Pasanen M. Cytochromes P450 2A6, 2E1, and 3A and production of proteinaldehyde adducts in the liver of patients with alcoholic and non-alcoholic liver diseases. J Hepatol 33: 893-901, 2000.
36. Novelli EL, Diniz YS, Galhardi CM, Ebaid GM, Rodrigues HG, Mani F, Fernandes AA, Cicogna AC, Novelli Filho JL. Anthropometrical parameters and markers of obesity in rats. Lab Anim 41: 111-119, 2007.
37. Ong WY, Jenner AM, Pan N, Ong CN, Halliwell B. Elevated oxidative stress, iron accumulation around microvessels and increased 4-hydroxynonenal immunostaining in zone 1 of the liver acinus in hypercholesterolemic rabbits. Free Radic Res 43: 241-249, 2009.
38. Reddy JK. Nonalcoholic steatosis and steatohepatitis. III. Peroxisomal α-oxidation, PPARγ, and steatohepatitis. Am J Physiol Gastrointest Liver Physiol 281: G1333-G1339, 2001.
39. Sakaguchi S, Takahashi S, Sasaki T, Kumagai T, Nagata K. Progression of alcoholic and non-alcoholic steatohepatitis: common metabolic aspects of innate immune system and oxidative stress. Drug Metab Pharmacokinet 26: 30-46, 2011.
40. Schaffert CS, Duryee MJ, Bennett RG, DeVeney AL, Tuma DJ, Olinga P, Easterling KC, Thiele GM, Klassen LW. Exposure of precision-cut rat liver slices to ethanol accelerates fibrogenesis. Am J Physiol Gastrointest Liver Physiol 299: G661-G668, 2010.
41. Serfaty L, Lemoine M. Definition and natural history of metabolic steatosis: clinical aspects of NAFLD, NASH and cirrhosis. Diabetes Metab 34: 634-637, 2008.
42. Starkel P, Leclercq IA. Animal models for the study of hepatic fibrosis. Best Pract Res Clin Gastroenterol 25: 319-333, 2011.
43. Takahashi Y, Soejima Y, Fukusato T. Animal models of nonalcoholic fatty liver disease/nonalcoholic steatohepatitis. World J Gastroenterol 18: 2300-2308, 2012.
44. Thiele GM, Duryee MJ, Willis MS, Tuma DJ, Radio SJ, Hunter CD, Schaffert CS, Klassen LW. Autoimmune hepatitis induced by syngeneic liver cytosolic proteins biotransformed by alcohol metabolites. Alcohol Clin Exp Res 34: 2126-2136, 2010.
45. Wigg AJ, Roberts-Thomson IC, Dymock RB, McCarthy PJ, Grose RH, Cummins AG. The role of small intestinal bacterial overgrowth, intestinal permeability, endotoxaemia, and tumour necrosis factor alpha in the pathogenesis of non-alcoholic steatohepatitis. Gut 48: 206-211, 2001.
46. Yang SQ, Lin HZ, Lane MD, Clemens M, Diehl AM. Obesity increases sensitivity to endotoxin liver injury: implications for the pathogenesis of steatohepatitis. Proc Natl Acad Sci USA 94: 2557-2562, 1997.
47. Zhu H, Jia Z, Misra H, Li YR. Oxidative stress and redox signaling mechanisms of alcoholic liver disease: updated experimental and clinical evidence. J Dig Dis 13: 133-142, 2012.