Exposure of precision-cut rat liver slices to ethanol accelerates fibrogenesis

American Journal of Physiology - Gastrointestinal and Liver Physiology

Volumn 299, Issue 3, 2010, Pages

  Schaffert, Courtney S ^a   Duryee, Michael J ^a   Bennett, Robert G ^a   DeVeney, Amy L ^a   Tuma, Dean J ^a   Olinga, Peter ^b   Easterling, Karen C ^a   Thiele, Geoffrey M ^a   Klassen, Lynell W ^a  

^a UNIVERSITY OF NEBRASKA MEDICAL CENTER   (United States)

^b UNIVERSITY OF GRONINGEN   (Netherlands)

Author keywords

Collagen; IL 6; Oxidative stress; Smooth muscle actin

Indexed keywords

ALCOHOL; COLLAGEN; COLLAGEN TYPE 1; GLUTATHIONE; INTERLEUKIN 6; SMOOTH MUSCLE ACTIN; THIOBARBITURIC ACID REACTIVE SUBSTANCE;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; CYTOKINE PRODUCTION; ENZYME LINKED IMMUNOSORBENT ASSAY; FIBROGENESIS; IMMUNOBLOTTING; IMMUNOHISTOCHEMISTRY; LIPID PEROXIDATION; LIVER FIBROSIS; LIVER INJURY; LIVER SLICE; MALE; MYOFIBROBLAST; NONHUMAN; OXIDATIVE STRESS; PRIORITY JOURNAL; RAT; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; ANIMAL; CHEMICALLY INDUCED DISORDER; CYTOLOGY; DRUG EFFECT; FIBROBLAST; GENE EXPRESSION REGULATION; GENETICS; LIVER; LIVER CIRRHOSIS; METABOLISM; PATHOLOGY; WISTAR RAT;

ANIMALS; ETHANOL; FIBROBLASTS; GENE EXPRESSION REGULATION; INTERLEUKIN-6; LIPID PEROXIDATION; LIVER; LIVER CIRRHOSIS; MALE; OXIDATIVE STRESS; RATS; RATS, WISTAR;

EID: 77957012704     PISSN: 01931857     EISSN: 15221547     Source Type: Journal    
DOI: 10.1152/ajpgi.00287.2009     Document Type: Article

References (40)

1. Albano E. Alcohol, oxidative stress and free radical damage. Proc Nutr Soc 65: 278-290, 2006.
2. Albano E. Oxidative mechanisms in the pathogenesis of alcoholic liver disease. Mol Aspects Med 29: 9-16, 2008.
3. Carlson TJ, Billings RE. Role of nitric oxide in the cytokine-mediated regulation of cytochrome P-450. Mol Pharmacol 49: 796-801, 1996.
4. Cederbaum AI, Wu D, Mari M, Bai J. CYP2E1-dependent toxicity and oxidative stress in HepG2 cells. Free Radic Biol Med 31: 1539-1543, 2001.
5. Choi DW, Kim SY, Kim SK, Kim YC. Factors involved in hepatic glutathione depletion induced by acute ethanol administration. J Toxicol Environ Health A 60: 459-469, 2000.
6. Clemens DL, Calisto LE, Sorrell MF, Tuma DJ. Ethanol metabolism results in a G2/M cell-cycle arrest in recombinant Hep G2 cells. Hepatology 38: 385-393, 2003.
7. Clemens DL, Halgard CM, Miles RR, Sorrell MF, Tuma DJ. Establishment of a recombinant hepatic cell line stably expressing alcohol dehydrogenase. Arch Biochem Biophys 321: 311-318, 1995.
8. Collins TJ. ImageJ for microscopy. Biotechniques 43: 25-30, 2007.
9. De Minicis S, Brenner DA. Oxidative stress in alcoholic liver disease: role of NADPH oxidase complex. J Gastroenterol Hepatol 23, Suppl 1: S98-S103, 2008.
10. Duryee MJ, Klassen LW, Freeman TL, Willis MS, Tuma DJ, Thiele GM. Lipopolysaccharide is a cofactor for malondialdehyde-acetaldehyde adduct-mediated cytokine/chemokine release by rat sinusoidal liver endothelial and Kupffer cells. Alcohol Clin Exp Res 28: 1931-1938, 2004.
11. Duryee MJ, Klassen LW, Thiele GM. Immunological response in alcoholic liver disease. World J Gastroenterol 13: 4938-4946, 2007.
12. Gao B. Cytokines, STATs and liver disease. Cell Mol Immunol 2: 92-100, 2005.
13. Greenwel P, Iraburu MJ, Reyes-Romero M, Meraz-Cruz N, Casado E, Solis-Herruzo JA, Rojkind M. Induction of an acute phase response in rats stimulates the expression of alpha 1(I) procollagen messenger ribonucleic acid in their livers. Possible role of interleukin-6. Lab Invest 72: 83-91, 1995.
14. Groneberg DA, Grosse-Siestrup C, Fischer A. In vitro models to study hepatotoxicity. Toxicol Pathol 30: 394-399, 2002.
15. Guo J, Friedman SL. Hepatic fibrogenesis. Semin Liver Dis 27: 413-426, 2007.
16. Han D, Hanawa N, Saberi B, Kaplowitz N. Mechanisms of liver injury. III. Role of glutathione redox status in liver injury. Am J Physiol Gastrointest Liver Physiol 291: G1-G7, 2006.
17. Hill DB, Marsano L, Cohen D, Allen J, Shedlofsky S, McClain CJ. Increased plasma interleukin-6 concentrations in alcoholic hepatitis. J Lab Clin Med 119: 547-552, 1992.
18. Horiguchi N, Wang L, Mukhopadhyay P, Park O, Jeong WI, Lafdil F, Osei-Hyiaman D, Moh A, Fu XY, Pacher P, Kunos G, Gao B. Cell type-dependent pro- and anti-inflammatory role of signal transducer and activator of transcription 3 in alcoholic liver injury. Gastroenterology 134: 1148-1158, 2008.
19. Iredale JP. Models of liver fibrosis: exploring the dynamic nature of inflammation and repair in a solid organ. J Clin Invest 117: 539-548, 2007.
20. Jin X, Zimmers TA, Perez EA, Pierce RH, Zhang Z, Koniaris LG. Paradoxical effects of short- and long-term interleukin-6 exposure on liver injury and repair. Hepatology 43: 474-484, 2006.
21. Klassen LW, Thiele GM, Duryee MJ, Schaffert CS, DeVeney AL, Hunter CD, Olinga P, Tuma DJ. An in vitro method of alcoholic liver injury using precision-cut liver slices from rats. Biochem Pharmacol 76: 426-436, 2008.
22. Latvala J, Hietala J, Koivisto H, Jarvi K, Anttila P, Niemela O. Immune responses to ethanol metabolites and cytokine profiles differentiate alcoholics with or without liver disease. Am J Gastroenterol 100: 1303-1310, 2005.
23. Li JT, Liao ZX, Ping J, Xu D, Wang H. Molecular mechanism of hepatic stellate cell activation and antifibrotic therapeutic strategies. J Gastroenterol 43: 419-428, 2008.
24. Nagata K, Suzuki H, Sakaguchi S. Common pathogenic mechanism in development progression of liver injury caused by non-alcoholic or alcoholic steatohepatitis. J Toxicol Sci 32: 453-468, 2007.
25. Ruifrok AC, Johnston DA. Quantification of histochemical staining by color deconvolution. Anal Quant Cytol Histol 23: 291-299, 2001.
26. Schaffert CS, Sorrell MF, Tuma DJ. Expression and cytoskeletal association of integrin subunits is selectively increased in rat perivenous hepatocytes after chronic ethanol administration. Alcohol Clin Exp Res 25: 1749-1757, 2001.
27. Schaffert CS, Todero SL, McVicker BL, Tuma PL, Sorrell MF, Tuma DJ. WIF-B cells as a model for alcohol-induced hepatocyte injury. Biochem Pharmacol 67: 2167-2174, 2004.
28. Siewert E, Bort R, Kluge R, Heinrich PC, Castell J, Jover R. Hepatic cytochrome P450 down-regulation during aseptic inflammation in the mouse is interleukin 6 dependent. Hepatology 32: 49-55, 2000.
29. Thiele GM, Duryee MJ, Freeman TL, Sorrell MF, Willis MS, Tuma DJ, Klassen LW. Rat sinusoidal liver endothelial cells (SECs) produce pro-fibrotic factors in response to adducts formed from the metabolites of ethanol. Biochem Pharmacol 70: 1593-1600, 2005.
30. Tuma DJ, Thiele GM, Xu D, Klassen LW, Sorrell MF. Acetaldehyde and malondialdehyde react together to generate distinct protein adducts in the liver during long-term ethanol administration. Hepatology 23: 872-880, 1996.
31. Tuma DJ, Zetterman RK, Sorrell MF. Inhibition of glycoprotein secretion by ethanol and acetaldehyde in rat liver slices. Biochem Pharmacol 29: 35-38, 1980.
32. Van de Bovenkamp M, Groothuis GM, Draaisma AL, Merema MT, Bezuijen JI, van Gils MJ, Meijer DK, Friedman SL, Olinga P. Precision-cut liver slices as a new model to study toxicity-induced hepatic stellate cell activation in a physiologic milieu. Toxicol Sci 85: 632-638, 2005.
33. Van de Bovenkamp M, Groothuis GM, Meijer DK, Olinga P. Liver fibrosis in vitro: cell culture models and precision-cut liver slices. Toxicol In Vitro 21: 545-557, 2007.
34. Van de Bovenkamp M, Groothuis GM, Meijer DK, Olinga P. Liver slices as a model to study fibrogenesis and test the effects of anti-fibrotic drugs on fibrogenic cells in human liver. Toxicol In Vitro 22: 771-778, 2008.
35. Vickers AE, Saulnier M, Cruz E, Merema MT, Rose K, Bentley P, Olinga P. Organ slice viability extended for pathway characterization: an in vitro model to investigate fibrosis. Toxicol Sci 82: 534-544, 2004.
36. Wasser S, Tan CE. Experimental models of hepatic fibrosis in the rat. Ann Acad Med Singapore 28: 109-111, 1999.
37. Weiler-Normann C, Herkel J, Lohse AW. Mouse models of liver fibrosis. Z Gastroenterol 45: 43-50, 2007.
38. Xu D, Thiele GM, Beckenhauer JL, Klassen LW, Sorrell MF, Tuma DJ. Detection of circulating antibodies to malondialdehyde-acetaldehyde adducts in ethanol-fed rats. Gastroenterology 115: 686-692, 1998.
39. Xu D, Thiele GM, Kearley ML, Haugen MD, Klassen LW, Sorrell MF, Tuma DJ. Epitope characterization of malondialdehyde-acetaldehyde adducts using an enzyme-linked immunosorbent assay. Chem Res Toxicol 10: 978-986, 1997.
40. Zima T, Kalousova M. Oxidative stress and signal transduction pathways in alcoholic liver disease. Alcohol Clin Exp Res 29: 110S-115S, 2005.