Silymarin suppresses hepatic stellate cell activation in a dietary rat model of non-alcoholic steatohepatitis: Analysis of isolated hepatic stellate cells

International Journal of Molecular Medicine

Volumn 30, Issue 3, 2012, Pages 473-479

  Kim, Mina ^a,b   Yang, Su Geun ^b   Kim, Joon Mi ^a   Lee, Jin Woo ^a   Kim, Young Soo ^a   Lee, Jung Il ^a  

^a Inha University School of Medicine   (South Korea)

^b Utah Inha DDS and Advanced Theapeutics Research Center   (South Korea)

Author keywords

Hepatic stellate cell; Insulin resistance; Methionine and choline deficient diet; Non alcoholic steatohepatitis; Silymarin

Indexed keywords

ALPHA SMOOTH MUSCLE ACTIN; CHOLINE; MESSENGER RNA; METHIONINE; PROCOLLAGEN; SILYMARIN; TRANSCRIPTION FACTOR NF E2; TUMOR NECROSIS FACTOR ALPHA;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; CELL ACTIVATION; CELL ISOLATION; CONTROLLED STUDY; DIET; DISEASE COURSE; FEEDING; FIBROGENESIS; HISTOLOGY; INSULIN RESISTANCE; LIVER CELL; LIVER PROTECTION; NONALCOHOLIC FATTY LIVER; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; RAT; STELLATE CELL;

ANIMALS; BODY WEIGHT; DIET; DISEASE MODELS, ANIMAL; DISEASE PROGRESSION; FATTY LIVER; HEPATIC STELLATE CELLS; INSULIN RESISTANCE; LIVER; LIVER CIRRHOSIS; MALE; MAP KINASE SIGNALING SYSTEM; NF-E2-RELATED FACTOR 2; ORGAN SIZE; PROTEIN TRANSPORT; RATS; SILYMARIN; TUMOR NECROSIS FACTOR-ALPHA;

EID: 84865176650     PISSN: 11073756     EISSN: 1791244X     Source Type: Journal    
DOI: 10.3892/ijmm.2012.1029     Document Type: Article

References (36)

1. Hubscher SG: Histologic assessment of non-alcoholic fatty liver disease. Histopathology 49: 450-465, 2006.
2. Harrison SA, Torgerson S and Hayashi PH: The natural history of nonalcoholic fatty liver disease: a clinical histopathological study. Am J Gastroenterol 98: 2042-2047, 2003. (Pubitemid 37185803)
3. Fassio F, Alvarez E, Dominguez N, Landeira G and Longo C: Natural history of nonalcoholic steatohepatitis: a longitudinal study of repeat liver biopsies. Hepatology 40: 820-826, 2004. (Pubitemid 39288114)
4. Adams LA, Sanderson S, Lindor KD and Angulo P: The histological course of nonalcoholic fatty liver disease: a longitudinal study of 103 patients with sequential liver biopsies. J Hepatol 42: 132-138, 2005. (Pubitemid 40038911)
5. Marceau P, Biron S, Hould FS, et al: Liver pathology and the metabolic syndrome X in severe obesity. J Clin Endocrinol Metab 84: 1513-1517, 1999. (Pubitemid 30644245)
6. Marchesini G, Brizi M, Bianchi G, et al: Nonalcoholic fatty liver disease: a feature of the metabolic syndrome. Diabetes 50: 1844-1850, 2001. (Pubitemid 33641605)
7. Marchesini G, Bugianesi E, Forlani G, et al: Nonalcoholic fatty liver, steatohepatitis, and the metabolic syndrome. Hepatology 37: 917-923, 2003. (Pubitemid 36397405)
8. Ota T, Takamura T, Kurita S, et al: Insulin resistance accelerates a dietary rat model of nonalcoholic steatohepatitis. Gastroenterology 132: 282-293, 2007. (Pubitemid 46108737)
9. Uno M, Kurita S, Miso H, et al: Tranilast, an antifibrogenic agent, ameliorates a dietary rat model of nonalcoholic steatohepatitis. Hepatology 48: 109-118, 2008. (Pubitemid 351975536)
10. Campos R, Garrido A, Guerra R and Valenzuela A: Silybin dihemisuccinate protects against glutathione depletion and lipid peroxidation induced by acetaminophen on rat liver. Planta Med 55: 417-419, 1989. (Pubitemid 19249808)
11. Leclercq IA, Farrell GC, Sempoux C, dela Pena A and Horsmans Y: Curcumin inhibits NF-kappaB activation and reduces the severity of experimental steatohepatitis in mice. J Hepatol 41: 926-934, 2004. (Pubitemid 39575659)
12. Halim AB, el-Ahmady O, Hassab-Allah S, Abdel-Galil F, Hafez Y and Darwish A: Biochemical effect of antioxidants on lipids and liver function in experimentally-induced liver damage. Ann Clin Biochem 34: 656-663, 1997. (Pubitemid 27480969)
13. Letteron P, Labbe G, Degott C, et al: Mechanism for the protective effects of silymarin against carbon tetrachloride-induced lipid peroxidation and hepatotoxicity in mice. Evidence that silymarin acts both as an inhibitor of metabolic activation and as a chain-breaking antioxidant. Biochem Pharmacol 39: 2027-2034, 1990. (Pubitemid 20187342)
14. Song Z, Song M, Lee DY, Liu Y, Deaciuc IV and McClain CJ: Silymarin prevents palmitate-induced lipotoxicity in HepG2 cells: involvement of maintenance of Akt kinase activation. Basic Clin Pharmacol Toxicol 101: 262-268, 2007. (Pubitemid 350194805)
15. Mourelle M, Muriel P, Favari L and Franco T: Prevention of CCl4-induced liver cirrhosis by silymarin. Fundam Clin Pharmacol 3: 183-191, 1989. (Pubitemid 19168196)
16. Tsai JH, Liu JY, Wu TT, et al: Effects of silymarin on the resolution of liver fibrosis induced by carbon tetrachloride in rats. J Viral Hepat 15: 508-514, 2008. (Pubitemid 351798986)
17. Kawano K, Hirashima T, Mori S, Saitoh Y, Kurosumi M and Natori T: Spontaneous long-term hyperglycemic rat with diabetic complications. Otsuka Long-Evans Tokushima Fatty (OLETF) strain. Diabetes 41: 1442-1428, 1992.
18. Dela Peña A, Leclercq IA, Field J, George J, Jones B and Farrell G: NF-κB activation, rather than TNF, mediates hepatic inflammation in a murine dietary model of steatohepatitis. Gastroenterology 129: 1663-1674, 2005. (Pubitemid 41597405)
19. Brunt EM: Nonalcoholic steatohepatitis: definition and pathology. Semin Liver Dis 21: 3-6, 2001.
20. Kirsch R, Clarkson V, Whephard EG, et al: Rodent nutritional model of non-alcoholic steatohepatitis: species, strain and sex differences studies. J Gastroenterol Hepatol 18: 1272-1282, 2003. (Pubitemid 37464002)
21. Kleiner DE, Brunt EM, Van Natta M, et al: Design and validation of a histological scoring system of nonalcoholic fatty liver disease. Hepatology 41: 1313-1321, 2005. (Pubitemid 40770284)
22. Yamazaki Y, Kakizaki S, Horiguchi N, et al: The role of the nuclear receptor constitutive androstane receptor in the pathogenesis of non-alcoholic steatohepatitis. Gut 56: 565-574, 2007. (Pubitemid 46580514)
23. Lee JI, Lee KS, Paik YH, et al: Apoptosis of hepatic stellate cells in carbon tetrachloride induced acute liver injury of the rat: analysis of isolated hepatic stellate cells. J Hepatol 39: 960-966, 2003. (Pubitemid 37523766)
24. Svegliati-Baroni G, D'Ambrosio L, Ferretti G, et al: Fibrogenic effect of oxidative stress on rat hepatic stellate cells. Hepatology 27: 720-726, 1998. (Pubitemid 28103548)
25. Nieto N, Dominguez-Rosales JA, Fontana L, et al: Rat hepatic stellate cells contribute to the acute-phase response with increased expression of alpha1(I) and alpha1(IV) collagens, tissue inhibitor of metalloproteinase-1 and matrix metalloproteinase-2 messenger RNAs. Hepatology 33: 597-607, 2001. (Pubitemid 32185222)
26. Schwabe RF, Schnabl B, Kweon YO and Brenner DA: CD40 activates NF-kappa B and c-Jun N-terminal kinase and enhances chemokine secretion on activated human hepatic stellate cells. J Immunol 166: 6812-6819, 2001. (Pubitemid 32471856)
27. Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method, Methods 25: 402-408, 2001. (Pubitemid 34164012)
28. Leclercq IA, Farrell GC, Field J, Bell DR, Gonzalez FJ and Robertson GR: CYP2E1 and CYP4A as microsomal catalysts of lipid peroxides in murine nonalcoholic steatohepatitis. J Clin Invest 105: 1067-1075, 2000. (Pubitemid 30217691)
29. Ip E, Farrell GC, Roberson G, Hall P, Kirsch R and Leclercq I: Central role of PPARalpha-dependent hepatic lipid turnover in dietary steatohepatitis in mice. Hepatology 38: 123-132, 2003. (Pubitemid 36775803)
30. Ligeret H, Brault A, Vallerand D, Haddad Y and Haddad PS: Antioxidant and mitochondrial protective effect of silibinin in cold preservation-warm reperfusion liver injury. J Ethnopharmacol 115: 507-514, 2008.
31. Lin MC, Kao SH, Chung PJ, Chan KC, Yang MY and Wang CJ: Improvement for high fat diet-induced hepatic injuries and oxidative stress by flavonoid-enriched extract from Nelumbo nucifera leaf. J Agric Food Chem 57: 5925-5932, 2009.
32. Haque M and Sanyal AJ: The metabolic abnormalities associated with non-alcoholic fatty liver disease. Best Pract Res Clin Gastroenterol 16: 709-731, 2002.
33. Sanyal AJ, Campbell-Sargent C, Mirshahi F, et al: Nonalcoholic steatohepatitis: association of insulin resistance and mitochondrial abnormalities. Gastroenterology 120: 1183-1192, 2001. (Pubitemid 32246672)
34. Zhan S and Rockey DC: Tumor necrosis factor α stimulates endothelin-1 synthesis in rat hepatic stellate cells in hepatic wound healing through a novel IKK/JKN pathway. Exp Cell Res 317: 1040-1048, 2011.
35. Gong P and Cederbaum AI: Nrf2 increased by CYP2E1 in rodent liver and HepG2 cells and protects against oxidative stress caused by CYP2E1. Hepatology 43: 144-153, 2006. (Pubitemid 43733601)
36. Nguyen T, Sherratt PJ and Pickett CB: Regulatory mechanisms controlling gene expression medicated by the antioxidant response element. Annu Rev Pharmacol Toxicol 43: 233-260, 2003. (Pubitemid 37372641)