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Volumn 51, Issue 1, 2014, Pages 257-269

Laser Capture Microdissection for the Investigative Pathologist

Author keywords

laser capture microdissection; molecular pathology; tissue processing

Indexed keywords

DNA; PARAFFIN; RNA;

EID: 84891708514     PISSN: 03009858     EISSN: 15442217     Source Type: Journal    
DOI: 10.1177/0300985813510533     Document Type: Article
Times cited : (23)

References (54)
  • 1
    • 0037390383 scopus 로고    scopus 로고
    • Evaluation of ethanol-fixed, paraffin-embedded tissues for proteomic applications
    • Ahram M,Flaig MJ,Gillespie JW,et al.Evaluation of ethanol-fixed, paraffin-embedded tissues for proteomic applications.Proteomics. 2003;3:413-421.
    • (2003) Proteomics , vol.3 , pp. 413-421
    • Ahram, M.1    Flaig, M.J.2    Gillespie, J.W.3
  • 2
    • 84861955398 scopus 로고    scopus 로고
    • Biorepositories: building better biobanks
    • Baker M.Biorepositories: building better biobanks.Nature. 2012;486:141-146.
    • (2012) Nature , vol.486 , pp. 141-146
    • Baker, M.1
  • 3
    • 84880061890 scopus 로고    scopus 로고
    • Biobanking of fresh-frozen human colon tissues: impact of tissue ex-vivo ischemia times and storage periods on RNA quality
    • Bao WG,Zhang X,Zhang JG,et al.Biobanking of fresh-frozen human colon tissues: impact of tissue ex-vivo ischemia times and storage periods on RNA quality.Ann Surg Oncol. 2013;20 (5): 1737-1744.
    • (2013) Ann Surg Oncol , vol.20 , Issue.5 , pp. 1737-1744
    • Bao, W.G.1    Zhang, X.2    Zhang, J.G.3
  • 4
    • 62449156047 scopus 로고    scopus 로고
    • Impact of thawing on RNA integrity and gene expression analysis in fresh frozen tissue
    • Botling J,Edlund K,Segersten U,et al.Impact of thawing on RNA integrity and gene expression analysis in fresh frozen tissue.Diagn Mol Pathol. 2009;18:44-52.
    • (2009) Diagn Mol Pathol , vol.18 , pp. 44-52
    • Botling, J.1    Edlund, K.2    Segersten, U.3
  • 5
    • 37549036339 scopus 로고    scopus 로고
    • One medicine-one pathology': are veterinary and human pathology prepared?
    • Cardiff RD WJ,Barthold SW.'One medicine-one pathology': are veterinary and human pathology prepared?.Lab Invest. 2008;88:18-26.
    • (2008) Lab Invest , vol.88 , pp. 18-26
    • Cardiff, R.D.W.J.1    Barthold, S.W.2
  • 6
    • 84859429097 scopus 로고    scopus 로고
    • EGFR and KRAS mutation analysis in cytologic samples of lung adenocarcinoma enabled by laser capture microdissection
    • Chowdhuri SR,Xi L,Pham TH,et al.EGFR and KRAS mutation analysis in cytologic samples of lung adenocarcinoma enabled by laser capture microdissection.Mod Pathol. 2012;25:548-555.
    • (2012) Mod Pathol , vol.25 , pp. 548-555
    • Chowdhuri, S.R.1    Xi, L.2    Pham, T.H.3
  • 7
    • 14844364789 scopus 로고    scopus 로고
    • Post-analysis follow-up and validation of microarray experiments
    • Chuaqui RF,Bonner RF,Best CJ,et al.Post-analysis follow-up and validation of microarray experiments.Nat Genet. 2002;32 (suppl): 509-514.
    • (2002) Nat Genet , vol.32 , Issue.SUPPL. , pp. 509-514
    • Chuaqui, R.F.1    Bonner, R.F.2    Best, C.J.3
  • 8
    • 77449115589 scopus 로고    scopus 로고
    • An optimized RNA extraction method from archival formalin-fixed paraffin-embedded tissue
    • Chung JY,Hewitt SM.An optimized RNA extraction method from archival formalin-fixed paraffin-embedded tissue.Methods Mol Biol. 2010;611:19-27.
    • (2010) Methods Mol Biol , vol.611 , pp. 19-27
    • Chung, J.Y.1    Hewitt, S.M.2
  • 9
    • 84859928466 scopus 로고    scopus 로고
    • A pressure cooking-based DNA extraction from archival formalin-fixed, paraffin-embedded tissue
    • Chung JY,Yi JM,Xie R,et al.A pressure cooking-based DNA extraction from archival formalin-fixed, paraffin-embedded tissue.Anal Biochem. 2012;425:128-134.
    • (2012) Anal Biochem , vol.425 , pp. 128-134
    • Chung, J.Y.1    Yi, J.M.2    Xie, R.3
  • 11
    • 84864966571 scopus 로고    scopus 로고
    • The mandate for a proper preservation in histopathological tissues
    • Comanescu M,Arsene D,Ardeleanu C,et al.The mandate for a proper preservation in histopathological tissues.Rom J Morphol Embryol. 2012;53:233-242.
    • (2012) Rom J Morphol Embryol , vol.53 , pp. 233-242
    • Comanescu, M.1    Arsene, D.2    Ardeleanu, C.3
  • 12
    • 77954373049 scopus 로고    scopus 로고
    • Immunoguided laser assisted microdissection techniques for DNA methylation analysis of archival tissue specimens
    • Eberle FC,Hanson JC,Killian JK,et al.Immunoguided laser assisted microdissection techniques for DNA methylation analysis of archival tissue specimens.J Mol Diagn. 2010;12:394-401.
    • (2010) J Mol Diagn , vol.12 , pp. 394-401
    • Eberle, F.C.1    Hanson, J.C.2    Killian, J.K.3
  • 13
    • 79953879650 scopus 로고    scopus 로고
    • Methylation profiling of mediastinal gray zone lymphoma reveals a distinctive signature with elements shared by classical Hodgkin's lymphoma and primary mediastinal large B-cell lymphoma
    • Eberle FC,Rodriguez-Canales J,Wei L,et al.Methylation profiling of mediastinal gray zone lymphoma reveals a distinctive signature with elements shared by classical Hodgkin's lymphoma and primary mediastinal large B-cell lymphoma.Haematologica. 2011;96:558-566.
    • (2011) Haematologica , vol.96 , pp. 558-566
    • Eberle, F.C.1    Rodriguez-Canales, J.2    Wei, L.3
  • 14
    • 80055119523 scopus 로고    scopus 로고
    • Laser capture microdissection of mammalian tissue
    • Edwards RA.Laser capture microdissection of mammalian tissue.J Vis Exp. 2007; (8): 309.
    • (2007) J Vis Exp , Issue.8 , pp. 309
    • Edwards, R.A.1
  • 15
    • 70350650795 scopus 로고    scopus 로고
    • Gene expression in Barrett's esophagus: laser capture versus whole tissue
    • El-Serag HB,Nurgalieva ZZ,Mistretta TA,et al.Gene expression in Barrett's esophagus: laser capture versus whole tissue.Scand J Gastroenterol. 2009;44:787-795.
    • (2009) Scand J Gastroenterol , vol.44 , pp. 787-795
    • El-Serag, H.B.1    Nurgalieva, Z.Z.2    Mistretta, T.A.3
  • 17
    • 66849138567 scopus 로고    scopus 로고
    • Quantitative RT-PCR gene expression analysis of laser microdissected tissue samples
    • Erickson HS,Albert PS,Gillespie JW,et al.Quantitative RT-PCR gene expression analysis of laser microdissected tissue samples.Nat Protoc. 2009;4:902-922.
    • (2009) Nat Protoc , vol.4 , pp. 902-922
    • Erickson, H.S.1    Albert, P.S.2    Gillespie, J.W.3
  • 18
    • 34547936920 scopus 로고    scopus 로고
    • Assessment of normalization strategies for quantitative RT-PCR using microdissected tissue samples
    • Erickson HS,Albert PS,Gillespie JW,et al.Assessment of normalization strategies for quantitative RT-PCR using microdissected tissue samples.Lab Invest. 2007;87:951-962.
    • (2007) Lab Invest , vol.87 , pp. 951-962
    • Erickson, H.S.1    Albert, P.S.2    Gillespie, J.W.3
  • 19
    • 77954674283 scopus 로고    scopus 로고
    • Influence of hypoxia induced by minimally invasive prostatectomy on gene expression: implications for biomarker analysis
    • Erickson HS,Josephson JW,Vira M,et al.Influence of hypoxia induced by minimally invasive prostatectomy on gene expression: implications for biomarker analysis.Am J Transl Res. 2010;2:210-222.
    • (2010) Am J Transl Res , vol.2 , pp. 210-222
    • Erickson, H.S.1    Josephson, J.W.2    Vira, M.3
  • 20
    • 14844318528 scopus 로고    scopus 로고
    • Pathology of the future: molecular profiling for targeted therapy
    • Espina V,Geho D,Mehta AI,et al.Pathology of the future: molecular profiling for targeted therapy.Cancer Invest. 2005;23:36-46.
    • (2005) Cancer Invest , vol.23 , pp. 36-46
    • Espina, V.1    Geho, D.2    Mehta, A.I.3
  • 21
    • 33747424780 scopus 로고    scopus 로고
    • Laser capture microdissection
    • Espina V,Milia J,Wu G,et al.Laser capture microdissection.Methods Mol Biol. 2006;319:213-229.
    • (2006) Methods Mol Biol , vol.319 , pp. 213-229
    • Espina, V.1    Milia, J.2    Wu, G.3
  • 22
    • 64649085620 scopus 로고    scopus 로고
    • Application of laser microdissection and reverse-phase protein microarrays to the molecular profiling of cancer signal pathway networks in the tissue microenvironment
    • Espina V,Wulfkuhle J,Liotta LA.Application of laser microdissection and reverse-phase protein microarrays to the molecular profiling of cancer signal pathway networks in the tissue microenvironment.Clin Lab Med. 2009;29:1-13.
    • (2009) Clin Lab Med , vol.29 , pp. 1-13
    • Espina, V.1    Wulfkuhle, J.2    Liotta, L.A.3
  • 24
    • 82355181814 scopus 로고    scopus 로고
    • Laser capture microdissection: Arcturus(XT) infrared capture and UV cutting methods
    • Gallagher RI,Blakely SR,Liotta LA,et al.Laser capture microdissection: Arcturus(XT) infrared capture and UV cutting methods.Methods Mol Biol. 2012;823:157-178.
    • (2012) Methods Mol Biol , vol.823 , pp. 157-178
    • Gallagher, R.I.1    Blakely, S.R.2    Liotta, L.A.3
  • 25
    • 0036173716 scopus 로고    scopus 로고
    • Evaluation of non-formalin tissue fixation for molecular profiling studies
    • Gillespie JW,Best CJ,Bichsel VE,et al.Evaluation of non-formalin tissue fixation for molecular profiling studies.Am J Pathol. 2002;160:449-457.
    • (2002) Am J Pathol , vol.160 , pp. 449-457
    • Gillespie, J.W.1    Best, C.J.2    Bichsel, V.E.3
  • 26
    • 0001372134 scopus 로고    scopus 로고
    • Thermal modeling of laser capture microdissection
    • Goldstein SR,McQueen PG,Bonner RF.Thermal modeling of laser capture microdissection.Appl Opt. 1998;37:7378-7391.
    • (1998) Appl Opt , vol.37 , pp. 7378-7391
    • Goldstein, S.R.1    McQueen, P.G.2    Bonner, R.F.3
  • 27
    • 84934437523 scopus 로고    scopus 로고
    • Laser capture microdissection for protein and NanoString RNA analysis
    • Golubeva Y,Salcedo R,Mueller C,et al.Laser capture microdissection for protein and NanoString RNA analysis.Methods Mol Biol. 2013;931:213-257.
    • (2013) Methods Mol Biol , vol.931 , pp. 213-257
    • Golubeva, Y.1    Salcedo, R.2    Mueller, C.3
  • 28
    • 38849181724 scopus 로고    scopus 로고
    • Contaminating cells alter gene signatures in whole organ versus laser capture microdissected tumors: a comparison of experimental breast cancers and their lymph node metastases
    • Harrell JC,Dye WW,Harvell DM,et al.Contaminating cells alter gene signatures in whole organ versus laser capture microdissected tumors: a comparison of experimental breast cancers and their lymph node metastases.Clin Exp Metastasis. 2008;25:81-88.
    • (2008) Clin Exp Metastasis , vol.25 , pp. 81-88
    • Harrell, J.C.1    Dye, W.W.2    Harvell, D.M.3
  • 29
    • 57349105253 scopus 로고    scopus 로고
    • Tissue handling and specimen preparation in surgical pathology: issues concerning the recovery of nucleic acids from formalin-fixed, paraffin-embedded tissue
    • Hewitt SM,Lewis FA,Cao Y,et al.Tissue handling and specimen preparation in surgical pathology: issues concerning the recovery of nucleic acids from formalin-fixed, paraffin-embedded tissue.Arch Pathol Lab Med. 2008;132:1929-1935.
    • (2008) Arch Pathol Lab Med , vol.132 , pp. 1929-1935
    • Hewitt, S.M.1    Lewis, F.A.2    Cao, Y.3
  • 30
    • 68449091888 scopus 로고    scopus 로고
    • Comparison of different methods to obtain and store liver biopsies for molecular and histological research
    • Hoffmann G,Ijzer J,Brinkhof B,et al.Comparison of different methods to obtain and store liver biopsies for molecular and histological research.Comp Hepatol. 2009;8:3.
    • (2009) Comp Hepatol , vol.8 , pp. 3
    • Hoffmann, G.1    Ijzer, J.2    Brinkhof, B.3
  • 31
    • 80055101440 scopus 로고    scopus 로고
    • Laser capture microdissection of Drosophila peripheral neurons
    • Iyer EP,Cox DN.Laser capture microdissection of Drosophila peripheral neurons.J Vis Exp. 2010;24:2016.
    • (2010) J Vis Exp , vol.24 , pp. 2016
    • Iyer, E.P.1    Cox, D.N.2
  • 32
    • 79960962251 scopus 로고    scopus 로고
    • Laser capture microdissection for analysis of gene expression in formalin-fixed paraffin-embedded tissue
    • Jiang R,Scott RS,Hutt-Fletcher LM.Laser capture microdissection for analysis of gene expression in formalin-fixed paraffin-embedded tissue.Methods Mol Biol. 2011;755:77-84.
    • (2011) Methods Mol Biol , vol.755 , pp. 77-84
    • Jiang, R.1    Scott, R.S.2    Hutt-Fletcher, L.M.3
  • 33
    • 66749148302 scopus 로고    scopus 로고
    • Approaching solid tumor heterogeneity on a cellular basis by tissue proteomics using laser capture microdissection and biological mass spectrometry
    • Johann DJ,Rodriguez-Canales J,Mukherjee S,et al.Approaching solid tumor heterogeneity on a cellular basis by tissue proteomics using laser capture microdissection and biological mass spectrometry.J Proteome Res. 2009;8:2310-2318.
    • (2009) J Proteome Res , vol.8 , pp. 2310-2318
    • Johann, D.J.1    Rodriguez-Canales, J.2    Mukherjee, S.3
  • 34
    • 84875813679 scopus 로고    scopus 로고
    • Guidelines for the care and use of laboratory animals in biomedical research
    • Jones-Bolin S.Guidelines for the care and use of laboratory animals in biomedical research.Curr Protoc Pharmacol. 2012;:.
    • (2012) Curr Protoc Pharmacol
    • Jones-Bolin, S.1
  • 35
    • 64649094395 scopus 로고    scopus 로고
    • Impact of sample acquisition and linear amplification on gene expression profiling of lung adenocarcinoma: laser capture micro-dissection cell-sampling versus bulk tissue-sampling
    • Klee EW,Erdogan S,Tillmans L,et al.Impact of sample acquisition and linear amplification on gene expression profiling of lung adenocarcinoma: laser capture micro-dissection cell-sampling versus bulk tissue-sampling.BMC Med Genomics. 2009;2:13.
    • (2009) BMC Med Genomics , vol.2 , pp. 13
    • Klee, E.W.1    Erdogan, S.2    Tillmans, L.3
  • 36
    • 79958779505 scopus 로고    scopus 로고
    • Comparison of formalin-free tissue fixatives: a proteomic study testing their application for routine pathology and research
    • Kothmaier H,Rohrer D,Stacher E,et al.Comparison of formalin-free tissue fixatives: a proteomic study testing their application for routine pathology and research.Arch Pathol Lab Med. 2011;135:744-752.
    • (2011) Arch Pathol Lab Med , vol.135 , pp. 744-752
    • Kothmaier, H.1    Rohrer, D.2    Stacher, E.3
  • 37
  • 38
    • 84871945956 scopus 로고    scopus 로고
    • Impact of ischemia and procurement conditions on gene expression in renal cell carcinoma
    • Liu NW,Sanford T,Srinivasan R,et al.Impact of ischemia and procurement conditions on gene expression in renal cell carcinoma.Clin Cancer Res. 2013;19:42-49.
    • (2013) Clin Cancer Res , vol.19 , pp. 42-49
    • Liu, N.W.1    Sanford, T.2    Srinivasan, R.3
  • 39
    • 80051733060 scopus 로고    scopus 로고
    • One-step preservation of phosphoproteins and tissue morphology at room temperature for diagnostic and research specimens
    • Mueller C,Edmiston KH,Carpenter C,et al.One-step preservation of phosphoproteins and tissue morphology at room temperature for diagnostic and research specimens.PLoS One. 2011;6:e23780.
    • (2011) PLoS One , vol.6
    • Mueller, C.1    Edmiston, K.H.2    Carpenter, C.3
  • 40
    • 74749090223 scopus 로고    scopus 로고
    • mRNA and micro-RNA expression analysis in laser-capture microdissected prostate biopsies: valuable tool for risk assessment and prevention trials
    • Nonn L,Vaishnav A,Gallagher L,et al.mRNA and micro-RNA expression analysis in laser-capture microdissected prostate biopsies: valuable tool for risk assessment and prevention trials.Exp Mol Pathol. 2010;88:45-51.
    • (2010) Exp Mol Pathol , vol.88 , pp. 45-51
    • Nonn, L.1    Vaishnav, A.2    Gallagher, L.3
  • 41
    • 39749160439 scopus 로고    scopus 로고
    • Proteomic analysis of laser-captured paraffin-embedded tissues: a molecular portrait of head and neck cancer progression
    • Patel V,Hood BL,Molinolo AA,et al.Proteomic analysis of laser-captured paraffin-embedded tissues: a molecular portrait of head and neck cancer progression.Clin Cancer Res. 2008;14:1002-1014.
    • (2008) Clin Cancer Res , vol.14 , pp. 1002-1014
    • Patel, V.1    Hood, B.L.2    Molinolo, A.A.3
  • 42
    • 8744315492 scopus 로고    scopus 로고
    • Comparison of snap freezing versus ethanol fixation for gene expression profiling of tissue specimens
    • Perlmutter MA,Best CJ,Gillespie JW,et al.Comparison of snap freezing versus ethanol fixation for gene expression profiling of tissue specimens.J Mol Diagn. 2004;6:371-377.
    • (2004) J Mol Diagn , vol.6 , pp. 371-377
    • Perlmutter, M.A.1    Best, C.J.2    Gillespie, J.W.3
  • 43
    • 80055103363 scopus 로고    scopus 로고
    • Obtaining high quality RNA from single cell populations in human postmortem brain tissue
    • Pietersen CY,Lim MP,Woo TU.Obtaining high quality RNA from single cell populations in human postmortem brain tissue.J Vis Exp. 2009; (30): 1444.
    • (2009) J Vis Exp , Issue.30 , pp. 1444
    • Pietersen, C.Y.1    Lim, M.P.2    Woo, T.U.3
  • 44
    • 79959264576 scopus 로고    scopus 로고
    • Why is it crucial to reintegrate pathology into cancer research?
    • Rodriguez-Canales J,Eberle FC,Jaffe ES,et al.Why is it crucial to reintegrate pathology into cancer research?.Bioessays. 2011;33:490-498.
    • (2011) Bioessays , vol.33 , pp. 490-498
    • Rodriguez-Canales, J.1    Eberle, F.C.2    Jaffe, E.S.3
  • 45
    • 84879570392 scopus 로고    scopus 로고
    • Optimal molecular profiling of tissue and tissue components: defining the best processing and microdissection methods for biomedical applications
    • Rodriguez-Canales J,Hanson JC,Hipp JD,et al.Optimal molecular profiling of tissue and tissue components: defining the best processing and microdissection methods for biomedical applications.Methods Mol Biol. 2013;980:61-120.
    • (2013) Methods Mol Biol , vol.980 , pp. 61-120
    • Rodriguez-Canales, J.1    Hanson, J.C.2    Hipp, J.D.3
  • 46
    • 33847407954 scopus 로고    scopus 로고
    • Identification of a unique epigenetic sub-microenvironment in prostate cancer
    • Rodriguez-Canales J,Hanson JC,Tangrea MA,et al.Identification of a unique epigenetic sub-microenvironment in prostate cancer.J Pathol. 2007;211:410-419.
    • (2007) J Pathol , vol.211 , pp. 410-419
    • Rodriguez-Canales, J.1    Hanson, J.C.2    Tangrea, M.A.3
  • 47
    • 77951782308 scopus 로고    scopus 로고
    • Protein pathway biomarker analysis of human cancer reveals requirement for upfront cellular-enrichment processing
    • Silvestri A,Colombatti A,Calvert VS,et al.Protein pathway biomarker analysis of human cancer reveals requirement for upfront cellular-enrichment processing.Lab Invest. 2010;90:787-796.
    • (2010) Lab Invest , vol.90 , pp. 787-796
    • Silvestri, A.1    Colombatti, A.2    Calvert, V.S.3
  • 48
    • 84875793461 scopus 로고    scopus 로고
    • Improved protocol for laser microdissection of human pancreatic islets from surgical specimens
    • Sturm D,Marselli L,Ehehalt F,et al.Improved protocol for laser microdissection of human pancreatic islets from surgical specimens.J Vis Exp. 2013; (71): 50231.
    • (2013) J Vis Exp , Issue.71 , pp. 50231
    • Sturm, D.1    Marselli, L.2    Ehehalt, F.3
  • 50
    • 79960908292 scopus 로고    scopus 로고
    • Effect of immunohistochemistry on molecular analysis of tissue samples: implications for microdissection technologies
    • Tangrea MA,Mukherjee S,Gao B,et al.Effect of immunohistochemistry on molecular analysis of tissue samples: implications for microdissection technologies.J Histochem Cytochem. 2011;59:591-600.
    • (2011) J Histochem Cytochem , vol.59 , pp. 591-600
    • Tangrea, M.A.1    Mukherjee, S.2    Gao, B.3
  • 51
    • 84878734869 scopus 로고    scopus 로고
    • The interference of cold ischemia time in the quality of total RNA from frozen tumor samples
    • Viana CR,Neto CS,Kerr LM,et al.The interference of cold ischemia time in the quality of total RNA from frozen tumor samples.Cell Tissue Bank. 2013;14 (2): 167-173.
    • (2013) Cell Tissue Bank , vol.14 , Issue.2 , pp. 167-173
    • Viana, C.R.1    Neto, C.S.2    Kerr, L.M.3
  • 52
    • 80055122463 scopus 로고    scopus 로고
    • Laser microdissection applied to gene expression profiling of subset of cells from the Drosophila wing disc
    • Vicidomini R,Tortoriello G,Furia M,et al.Laser microdissection applied to gene expression profiling of subset of cells from the Drosophila wing disc.J Vis Exp. 2010; (38): 1895.
    • (2010) J Vis Exp , Issue.38 , pp. 1895
    • Vicidomini, R.1    Tortoriello, G.2    Furia, M.3
  • 53
    • 79955680588 scopus 로고    scopus 로고
    • Factors influencing the degradation of archival formalin-fixed paraffin-embedded tissue sections
    • Xie R,Chung JY,Ylaya K,et al.Factors influencing the degradation of archival formalin-fixed paraffin-embedded tissue sections.J Histochem Cytochem. 2011;59:356-365.
    • (2011) J Histochem Cytochem , vol.59 , pp. 356-365
    • Xie, R.1    Chung, J.Y.2    Ylaya, K.3
  • 54
    • 84860667323 scopus 로고    scopus 로고
    • MicroRNA analysis of microdissected normal squamous esophageal epithelium and tumor cells
    • Zhu L,Yan W,Rodriguez-Canales J,et al.MicroRNA analysis of microdissected normal squamous esophageal epithelium and tumor cells.Am J Cancer Res. 2011;1:574-584.
    • (2011) Am J Cancer Res , vol.1 , pp. 574-584
    • Zhu, L.1    Yan, W.2    Rodriguez-Canales, J.3


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