Laser capture Microdissection of Drosophila peripheral neurons

Journal of Visualized Experiments

Volumn , Issue 39, 2010, Pages

  Iyer, Eswar Prasad R ^a   Cox, Daniel N ^a  

^a GEORGE MASON UNIVERSITY   (United States)

Author keywords

Cell isolation; Dendritic arborization neurons; Drosophila; Issue 39; Jove neuroscience; Laser capture microdissection (lcm); Microarray; Neuron; Peripheral nervous system (pns); Qrt pcr; Rna

Indexed keywords

EID: 80055101440     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/2016     Document Type: Article

References (23)

1. Corty, M.M., Matthews, B.J. and Grueber, W.B. Molecules and mechanisms of dendrite development in Drosophila. Development 136, 1049-1061 (2009).
2. Parrish, J.Z., Emoto, K., Kim, M.D., and Jan, Y.N. Mechanisms that regulate establishment, maintenance, and remodeling of dendritic fields. Annu. Rev. Neurosci. 30, 399-423 (2007).
3. Wang, X., Starz-Gaiano, M., Bridges, T. and Montell, D. Purification of specific cell populations from Drosophila tissues by magnetic bead sorting, for use in gene expression profiling. Nature Protocols doi:doi:10.1038/nprot.2008.28
4. Iyer, E.P.R., Iyer, S.C., Sulkowski, M.J., and Cox, D.N. Isolation and purification of Drosophila peripheral neurons by magnetic bead sorting. JoVE 34, http://www.jove.com/index/details.stp?id=1599, doi: 10.3791/1599
5. Tirouvanziam, R., Davidson, C.J., Lipsick, J.S. and Herzenberg, L.A. Fluorescence-activated cell sorting (FACS) of Drosophila hemocytes reveals important functional similarities to mammalian leukocytes. Proc. Natl. Acad. Sci. 101, 2912-2917 (2004).
6. Shigenobu, S., Arita, K., Kitadate, Y., Noda, C. and Kobayashi, S. Isolation of germline cells from Drosophila embryos by flow cytometry. Dev. Growth Differ. 48, 49-57 (2006).
7. Reeves, N. and Posakony, J.W. Genetic programs activated by proneural proteins in the developing Drosophila PNS. Dev. Cell 8, 413-425 (2005).
8. Jinushi-Nakao, S. et al. Knot/Collier and Cut control different aspects of dendrite cytoskeleton and synergize to define final arbor shape. Neuron 56, 963-978 (2007).
9. Yang, Z., Edenberg, H.J. and Davis, R.L. Isolation of mRNA from specific tissues of Drosophila by mRNA tagging. Nucl. Acids Res. 33, e148 (2005).
10. Appay, V. et al. Sensitive Gene Expression Profiling of Human T Cell Subsets Reveals Parallel Post-Thymic Differentiation for CD4+ and CD8+ Lineages. J. Immunol. 179, 7406-7414 (2007).
11. Ginzinger, D.G. Gene quantification using real-time quantitative PCR: An emerging technology hits the mainstream. Exp. Hematol. 30, 503-512 (2002).
12. Keays, K.M., Owens, G.P., Ritchie, A.M., Gilden, D.H. and Burgoon, M.P. Laser capture microdissection and single-cell RT-PCR without RNA purification. J. Immunol. Methods 302, 90-98 (2005).
13. Volgin, D.V., Swan, J. and Kubin, L. Single-cell RT-PCR gene expression profiling of acutely dissociated and immunocytochemically identified central neurons. J. Neurosci. Methods 136, 229-236 (2004).
14. Emmert-Buck, M.R. et al. Laser capture microdissection. Science 274, 998-1001 (1996).
15. Xiao, W. et al. Gene expression profiling in embryonic mouse lenses. Mol. Vis. 12, 1692-1698 (2006).
16. Scharschmidt, T. et al. Analysis of human osteoarthritic connective tissue by laser capture microdissection and QRT-PCR. Connect. Tissue Res. 48, 316-323 (2007).
17. Spletter, M.L. et al. Lola regulates Drosophila olfactory projection neuron identity and targeting specificity. Neural Dev. 2, 14 (2007).
18. Hoopfer, E.D., Penton, A., Watts, R.J. and Luo, L. Genomic analysis of Drosophila neuronal remodeling: a role for the RNA-binding protein Boule as a negative regulator of axon pruning. J. Neurosci. 28, 6092-6103 (2008).
19. Lee, T. and Luo, L. Mosaic analysis with a repressible cell marker for studies of gene function in neuronal morphogenesis. Neuron 22, 451-461 (1999).
20. Grueber, W.B. et al. Projections of Drosophila multidendritic neurons in the central nervous system: links with peripheral dendrite morphology. Development 134, 55-64 (2007).
21. Song, W., Onishi, M., Jan, L.Y. and Jan, Y.N. Peripheral multidendritic sensory neurons are necessary for rhythmic locomotion behavior in Drosophila larvae. Proc. Natl. Acad. Sci. USA 104, 5199-5204 (2007).
22. Espina, V. et al. Laser capture microdissection. Nat. Prot. 1, 586-603 (2006).
23. Livak, K.J. and Schmittgen, T.D. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods 25, 402-408 (2001).