Laser capture microdissection: Arcturus XT infrared capture and UV cutting methods

Methods in Molecular Biology

Volumn 823, Issue , 2012, Pages 157-178

  Gallagher, Rosa I ^a   Blakely, Steven R ^b   Liotta, Lance A ^a   Espina, Virginia ^a  

^a GEORGE MASON UNIVERSITY   (United States)

^b APPLIED BIOSYSTEMS   (United States)

Author keywords

Cancer; DNA; Infrared; Laser capture microdissection; Molecularprofi ling; Protein; RNA; Tissue; Tissue heterogeneity; Ultraviolet

Indexed keywords

PROTEIN;

ANIMAL; ARTICLE; HUMAN; INFRARED RADIATION; LASER CAPTURE MICRODISSECTION; METHODOLOGY; STAINING; ULTRAMICROTOMY; ULTRAVIOLET RADIATION;

ANIMALS; CRYOULTRAMICROTOMY; HUMANS; INFRARED RAYS; LASER CAPTURE MICRODISSECTION; PROTEINS; STAINING AND LABELING; ULTRAVIOLET RAYS;

EID: 82355181814     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-60327-216-2_11     Document Type: Article

References (30)

1. Wulfkuhle, J. D., Speer, R., Pierobon, M., Laird, J., Espina, V., Deng, J. et al. (2008) Multiplexed cell signaling analysis of human breast cancer applications for personalized therapy. J Proteome Res 7, 1508 - 17.
2. Petricoin, E. F., 3rd, Espina, V., Araujo, R. P., Midura, B., Yeung, C., Wan, X. et al. (2007) Phosphoprotein pathway mapping: Akt/mammalian target of rapamycin activation is negatively associated with childhood rhabdomyosarcoma survival. Cancer Res 67, 3431 - 40. (Pubitemid 46724884)
3. Petricoin, E. F., 3rd, Bichsel, V. E., Calvert, V. S., Espina, V., Winters, M., Young, L. et al. (2005) Mapping molecular networks using proteomics: a vision for patient-tailored combination therapy. J Clin Oncol 23, 3614 - 21.
4. Wulfkuhle, J. D., Sgroi, D. C., Krutzsch, H., McLean, K., McGarvey, K., Knowlton, M. et al. (2002) Proteomics of human breast ductal carcinoma in situ. Cancer Res 62, 6740 - 9. (Pubitemid 35364146)
5. Nakazono, M., Qiu, F., Borsuk, L. A., Schnable, P. S. (2003) Laser-capture microdissection, a tool for the global analysis of gene expression in specific plant cell types: identification of genes expressed differentially in epidermal cells or vascular tissues of maize. Plant Cell 15, 583 - 96. (Pubitemid 36339575)
6. Ma, X. J., Dahiya, S., Richardson, E. A., Erlander, M., Sgroi, D. C. (2009) Gene expression profiling of tumor microenvironment during breast cancer progression. Breast Cancer Res 11, R7.
7. Emmert-Buck, M. R., Bonner, R. F., Smith, P. D., Chuaqui, R. F., Zhuang, Z., Goldstein, S. R. et al. (1996) Laser capture microdissection. Science 274, 998 - 1001. (Pubitemid 26398425)
8. Bonner, R. F., Emmert-Buck, M., Cole, K., Pohida, T., Chuaqui, R., Goldstein, S. et al. (1997) Laser capture microdissection: molecular analysis of tissue. Science 278, 1481,1483.
9. Angeles, G., Berrio-Sierra, J., Joseleau, J. P., Lorimier, P., Lefebvre, A., Ruel, K. (2006) Preparative laser capture microdissection and single-pot cell wall material preparation: a novel method for tissue-specific analysis. Planta 224, 228 - 32. (Pubitemid 43800717)
10. Gallup, J. M., Kawashima, K., Lucero, G., Ackermann, M. R. (2005) New quick method for isolating RNA from laser captured cells stained by immunofl uorescent immunohistochemistry; RNA suitable for direct use in fl uorogenic TaqMan one-step real-time RT-PCR. Biol Proced Online 7, 70 - 92. (Pubitemid 41358163)
11. Mouledous, L., Hunt, S., Harcourt, R., Harry, J., Williams, K. L., Gutstein, H. B. (2003) Navigated laser capture microdissection as an alternative to direct histological staining for proteomic analysis of brain samples. Proteomics 3, 610 - 5. (Pubitemid 36570252)
12. Nakamura, N., Ruebel, K., Jin, L., Qian, X., Zhang, H., Lloyd, R. V. (2007) Laser capture microdissection for analysis of single cells. Methods Mol Med 132, 11 - 8.
13. VanMeter, A. J., Rodriguez, A. S., Bowman, E. D., Jen, J., Harris, C. C., Deng, J. et al. (2008) Laser capture microdissection and protein microarray analysis of human non-small cell lung cancer: differential epidermal growth factor receptor (EGPR) phosphorylation events associated with mutated EGFR compared with wild type. Mol Cell Proteomics 7, 1902 - 24.
14. Wong, M. H., Saam, J. R., Stappenbeck, T. S., Rexer, C. H., Gordon, J. I. (2000) Genetic mosaic analysis based on Cre recombinase and navigated laser capture microdissection. Proc Natl Acad Sci U S A 97, 12601 - 6.
15. Kennedy, J., Katsuta, H., Jung, M. H., Marselli, L., Goldfine, A. B., Balis, U. J. et al. (2010) Protective unfolded protein response in human pancreatic beta cells transplanted into mice. PLoS One 5, e11211.
16. Cha, S., Imielinski, M. B., Rejtar, T., Richardson, E. A., Thakur, D., Sgroi, D. C. et al. (2010) In situ proteomic analysis of human breast cancer epithelial cells using laser capture microdissection: annotation by protein set enrichment analysis and gene ontology. Mol Cell Proteomics 9, 2529 - 44.
17. Kolble, K. (2000) The LEICA microdissection system: design and applications. J Mol Med 78, B24-5.
18. Micke, P., Ostman, A., Lundeberg, J., Ponten, F. (2005) Laser-assisted cell microdissection using the PALM system. Methods Mol Biol 293, 151 - 66.
19. Schutze, K., Posl, H., Lahr, G. (1998) Laser micromanipulation systems as universal tools in cellular and molecular biology and in medicine. Cell Mol Biol (Noisy-le-grand) 44, 735 - 46.
20. Schermelleh, L., Thalhammer, S., Heckl, W., Posl, H., Cremer, T., Schutze, K. et al. (1999) Laser microdissection and laser pressure catapulting for the generation of chromosome-specific paint probes. Biotechniques 27, 362 - 7. (Pubitemid 29382405)
21. Espina, V., Edmiston, K. H., Heiby, M., Pierobon, M., Sciro, M., Merritt, B. et al. (2008) A portrait of tissue phosphoprotein stability in the clinical tissue procurement process. Mol Cell Proteomics 7, 1998 - 2018.
22. Botling, J., Edlund, K., Segersten, U., Tahmasebpoor, S., Engstrom, M., Sundstrom, M. et al. (2009) Impact of Thawing on RNA Integrity and Gene Expression Analysis in Fresh Frozen Tissue. Diagn Mol Pathol
23. Micke, P., Ohshima, M., Tahmasebpoor, S., Ren, Z. P., Ostman, A., Ponten, F. et al. (2006) Biobanking of fresh frozen tissue: RNA is stable in nonfixed surgical specimens. Lab Invest 86, 202 - 11. (Pubitemid 43117599)
24. Xiang, C. C., Mezey, E., Chen, M., Key, S., Ma, L., Brownstein, M. J. (2004) Using DSP, a reversible cross-linker, to fi x tissue sections for immunostaining, microdissection and expression profiling. Nucleic Acids Res 32, e185.
25. Espina, V., Wulfkuhle, J. D., Calvert, V. S., VanMeter, A., Zhou, W., Coukos, G. et al. (2006) Laser-capture microdissection. Nat Protoc 1, 586 - 603.
26. Arcturus XT user guide: Flexible and Modular Laser Capture Microdissection, Rev. A. Molecular Devices Corporation, Sunnyvale, CA, 2007.
27. Iyer, E. P., Cox, D. N. (2010) Laser capture microdissection of Drosophila peripheral neurons. J Vis Exp 39, e2016, doi: 10.3791/2016.
28. Kiernan, J. (2008) Histological staining in one or two colours, in Histological and Histochemical Methods (ed.), Scion, Oxfordshire, pp. 146-147.
29. Buckanovich, R. J., Sasaroli, D., O'Brien- Jenkins, A., Botbyl, J., Conejo-Garcia, J. R., Benencia, F. et al. (2006) Use of immuno- LCM to identify the in situ expression profi le of cellular constituents of the tumor microenvironment. Cancer Biol Ther 5, 635 - 42. (Pubitemid 44024718)
30. Agar, N. S., Halliday, G. M., Barnetson, R. S., Jones, A. M. (2003) A novel technique for the examination of skin biopsies by laser capture microdissection. J Cutan Pathol 30, 265 - 70. (Pubitemid 36555135)