Daily nutritional dose supplementation with antioxidant nutrients and phytochemicals improves DNA and LDL stability: A double-blind, randomized, and placebo-controlled trial

Nutrients

Volumn 5, Issue 12, 2013, Pages 5218-5232

  Kim, You Jin ^a   Ahn, Yoon Hee ^a   Lim, Yeni ^a   Kim, Ji Yeon ^b   Kim, Joohee ^a   Kwon, Oran ^a  

^a Ewha Womans University   (South Korea)

^b Seoul National University of Science and Technology   (South Korea)

Author keywords

Human intervention; Multi micronutrient supplement; Nutritional dose; Phytochemicals

Indexed keywords

ALPHA TOCOPHEROL; ANTIOXIDANT; ASCORBIC ACID; BETA CAROTENE; BIOTIN; CALCIUM; CATALASE; CHROMIUM; COPPER; CYANOCOBALAMIN; DOUBLE X; FOLIC ACID; GLUCOSE; GLUTATHIONE PEROXIDASE; IODINE; IRON; MAGNESIUM; MANGANESE; NICOTINIC ACID; PANTOTHENIC ACID; PLACEBO; PLANT MEDICINAL PRODUCT; PYRIDOXINE; REACTIVE OXYGEN METABOLITE; RETINOL; RIBOFLAVIN; SELENIUM; SUPEROXIDE DISMUTASE; THIAMINE; UNCLASSIFIED DRUG; UNINDEXED DRUG; VITAMIN D; VITAMIN K GROUP; ZINC;

ADULT; AGED; ARTICLE; ASSAY; COLORIMETRY; COMET ASSAY; CONTROLLED STUDY; DIET SUPPLEMENTATION; DIETARY REFERENCE INTAKE; DNA DAMAGE; DNA PURIFICATION; DOUBLE BLIND PROCEDURE; ELISA KIT; FEMALE; FLUORESCENCE MICROSCOPE; FOLIC ACID BLOOD LEVEL; HUMAN; HUMAN EXPERIMENT; IMMUNOASSAY ANALYZER; KOLMOGOROV SMIRNOV TEST; MALE; NORMAL HUMAN; OUTCOME ASSESSMENT; OXIDATIVE STRESS; RANDOMIZED CONTROLLED TRIAL;

ADULT; AGED; ANTIOXIDANTS; BLOOD PRESSURE; BODY MASS INDEX; CATALASE; CHOLESTEROL, HDL; CHOLESTEROL, LDL; COMET ASSAY; DIETARY SUPPLEMENTS; DNA DAMAGE; DOSE-RESPONSE RELATIONSHIP, DRUG; DOUBLE-BLIND METHOD; FEMALE; FRUIT; GLUTATHIONE PEROXIDASE; HUMANS; LIPOPROTEINS, LDL; MALE; MICRONUTRIENTS; MIDDLE AGED; PHYTOCHEMICALS; SUPEROXIDE DISMUTASE; TREATMENT OUTCOME; TRIGLYCERIDES; VEGETABLES;

EID: 84890542986     PISSN: None     EISSN: 20726643     Source Type: Journal    
DOI: 10.3390/nu5125218     Document Type: Article

References (39)

1. Harman, D. Free radical theory of aging: An update: Increasing the functional life span. Ann. N. Y. Acad. Sci. 2006, 1067, 10-21.
2. Ames, B.N. Endogenous DNA damage as related to cancer and aging. Mutat. Res. 1989, 214, 41-46.
3. Machlin, L.J.; Bendich, A. Free radical tissue damage: Protective role of antioxidant nutrients. FASEB J. 1987, 1, 441-445.
4. Omenn, G.S.; Goodman, G.E.; Thornquist, M.D.; Balmes, J.; Cullen, M.R.; Glass, A.; Keogh, J.P.; Meyskens, F.L., Jr.; Valanis, B.; Williams, J.H., et al. Risk factors for lung cancer and for intervention effects in CARET, the β-Carotene and Retinol Efficacy Trial. J. Natl. Cancer Inst. 1996, 88, 1550-1559.
5. Ristow, M.; Zarse, K.; Oberbach, A.; Kloting, N.; Birringer, M.; Kiehntopf, M.; Stumvoll, M.; Kahn, C.R.; Bluher, M. Antioxidants prevent health-promoting effects of physical exercise in humans. Proc. Natl. Acad. Sci. USA 2009, 106, 8665-8670.
6. Liu, C.; Wang, X.D.; Bronson, R.T.; Smith, D.E.; Krinsky, N.I.; Russell, R.M. Effects of physiological versus pharmacological β-carotene supplementation on cell proliferation and histopathological changes in the lungs of cigarette smoke-exposed ferrets. Carcinogenesis 2000, 21, 2245-2253.
7. Rhee, S.G. Cell signaling. H2O2, a necessary evil for cell signaling. Science 2006, 312, 1882-1883.
8. Salganik, R.I. The benefits and hazards of antioxidants: Controlling apoptosis and other protective mechanisms in cancer patients and the human population. J. Am. Coll. Nutr. 2001, 20, 464S-472S; discussion 473S-475S.
9. Dauchet, L.; Amouyel, P.; Hercberg, S.; Dallongeville, J. Fruit and vegetable consumption and risk of coronary heart disease: A meta-analysis of cohort studies. J. Nutr. 2006, 136, 2588-2593.
10. Liu, R.H. Health benefits of fruit and vegetables are from additive and synergistic combinations of phytochemicals. Am. J. Clin. Nutr. 2003, 78, 517S-520S.
11. Goupy, P.; Hugues, M.; Boivin, P.; Amiot, M.J. Antioxidant composition and activity of barley (Hordeum vulgare) and malt extracts and of isolated phenolic compounds. J. Sci. Food Agric. 1999, 79, 1625-1634.
12. Walker, R.B.; Everette, J.D. Comparative reaction rates of various antioxidants with ABTS radical cation. J. Agric. Food Chem. 2009, 57, 1156-1161.
13. Huang, D.; Ou, B.; Hampsch-Woodill, M.; Flanagan, J.A.; Prior, R.L. High-throughput assay of oxygen radical absorbance capacity (ORAC) using a multichannel liquid handling system coupled with a microplate fluorescence reader in 96-well format. J. Agric. Food Chem. 2002, 50, 4437-4444.
14. Benzie, I.F.; Strain, J.J. The ferric reducing ability of plasma (FRAP) as a measure of "antioxidant power": The FRAP assay. Anal. Biochem. 1996, 239, 70-76.
15. Singleton, V.L.; Orthofer, R.; Lamuela-Raventós, R.M. Analysis of totalphenols and otheroxidationsubstrates and antioxidants by means of folin-ciocalteu reagent. Methods Enzymol. 1999, 299, 152-178.
16. Kim, J.Y.; Yang, Y.J.; Yang, Y.K.; Oh, S.Y.; Hong, Y.C.; Lee, E.K.; Kwon, O. Diet quality scores and oxidative stress in Korean adults. Eur. J. Clin. Nutr. 2011, 65, 1271-1278.
17. Schulz, K.F.; Altman, D.G.; Moher, D.; Group, C. CONSORT 2010 statement: Updated guidelines for reporting parallel group randomized trials. Ann. Intern. Med. 2010, 152, 726-732.
18. Olive, P.L.; Banath, J.P. The Comet Assay: A method to measure DNA damage in individual Cells. Nat. Protoc. 2006, 1, 23-29.
19. Lee, J.H. Yonsei University, Seoul, Korea. Unpublished work, 2004.
20. Block, G.; Dietrich, M.; Norkus, E.P.; Morrow, J.D.; Hudes, M.; Caan, B.; Packer, L. Factors associated with oxidative stress in human populations. Am. J. Epidemiol. 2002, 156, 274-285.
21. Holt, E.M.; Steffen, L.M.; Moran, A.; Basu, S.; Steinberger, J.; Ross, J.A.; Hong, C.P.; Sinaiko, A.R. Fruit and vegetable consumption and its relation to markers of inflammation and oxidative stress in adolescents. J. Am. Diet. Assoc. 2009, 109, 414-421.
22. Liu, R.H. Potential synergy of phytochemicals in cancer prevention: Mechanism of action. J. Nutr. 2004, 134, 3479S-3485S.
23. Dotan, Y.; Lichtenberg, D.; Pinchuk, I. Lipid peroxidation cannot be used as a universal criterion of oxidative stress. Prog. Lipid Res. 2004, 43, 200-227.
24. Jackson, A.L.; Loeb, L.A. The contribution of endogenous sources of DNA damage to the multiple mutations in cancer. Mutat. Res. 2001, 477, 7-21.
25. Bocker, W.; Bauch, T.; Muller, W.U.; Streffer, C. Image analysis of comet assay measurements. Int. J. Radiat. Biol. 1997, 72, 449-460.
26. De Boeck, M.; Touil, N.; De Visscher, G.; Vande, P.A.; Kirsch-Volders, M. Validation and implementation of an internal standard in comet assay analysis. Mutat. Res. 2000, 469, 181-197.
27. Heaton, P.R.; Ransley, R.; Charlton, C.J.; Mann, S.J.; Stevenson, J.; Smith, B.H.; Rawlings, J.M.; Harper, E.J. Application of single-cell gel electrophoresis (comet) assay for assessing levels of DNA damage in canine and feline leukocytes. J. Nutr. 2002, 132, 1598S-1603S.
28. Shimoi, K.; Kasai, H.; Yokota, N.; Toyokuni, S.; Kinae, N. Comparison between high-performance liquid chromatography and enzyme-linked immunosorbent assay for the determination of 8-hydroxy-2′-deoxyguanosine in human urine. Cancer Epidemiol. Biomark. Prev. 2002, 11, 767-770.
29. Best, T.M.; Fiebig, R.; Corr, D.T.; Brickson, S.; Ji, L. Free radical activity, antioxidant enzyme, and glutathione changes with muscle stretch injury in rabbits. J. Appl. Physiol. 1999, 87, 74-82.
30. Cheng, G.W.; Wu, H.L.; Huang, Y.L. Simultaneous determination of malondialdehyde and ofloxacin in plasma using an isocratic high-performance liquid chromatography/fluorescence detection system. Anal. Chim. Acta 2008, 616, 230-234.
31. Song, M.F.; Li, Y.S.; Ootsuyama, Y.; Kasai, H.; Kawai, K.; Ohta, M.; Eguchi, Y.; Yamato, H.; Matsumoto, Y.; Yoshida, R.; et al. Urea, the most abundant component in urine, cross-reacts with a commercial 8-OH-dG ELISA kit and contributes to overestimation of urinary 8-OH-dG. Free Radic. Biol. Med. 2009, 47, 41-46.
32. Ristow, M.; Zarse, K. How increased oxidative stress promotes longevity and metabolic health: The concept of mitochondrial hormesis (mitohormesis). Exp. Gerontol. 2010, 45, 410-418.
33. Song, Y.; Cook, N.R.; Albert, C.M.; Van Denburgh, M.; Manson, J.E. Effects of vitamins C and E and β-carotene on the risk of type 2 diabetes in women at high risk of cardiovascular disease: A randomized controlled trial. Am. J. Clin. Nutr. 2009, 90, 429-437.
34. Lin, J.; Cook, N.R.; Albert, C.; Zaharris, E.; Gaziano, J.M.; Van Denburgh, M.; Buring, J.E.; Manson, J.E. Vitamins C and E and β carotene supplementation and cancer risk: A randomized controlled trial. J. Natl. Cancer Inst. 2009, 101, 14-23.
35. Lippman, S.M.; Klein, E.A.; Goodman, P.J.; Lucia, M.S.; Thompson, I.M.; Ford, L.G.; Parnes, H.L.; Minasian, L.M.; Gaziano, J.M.; Hartline, J.A., et al. Effect of selenium and vitamin E on risk of prostate cancer and other cancers: The Selenium and Vitamin E Cancer Prevention Trial (SELECT). JAMA 2009, 301, 39-51.
36. Lawenda, B.D.; Kelly, K.M.; Ladas, E.J.; Sagar, S.M.; Vickers, A.; Blumberg, J.B. Should supplemental antioxidant administration be avoided during chemotherapy and radiation therapy? J. Natl. Cancer Inst. 2008, 100, 773-783.
37. Milic, M.; Rozgaj, R.; Kasuba, V.; Orescanin, V.; Balija, M.; Jukic, I. Correlation between folate and vitamin B12 and markers of DNA stability in healthy men: Preliminary results. Acta Biochim. Pol. 2010, 57, 339-345.
38. Basten, G.P.; Duthie, S.J.; Pirie, L.; Vaughan, N.; Hill, M.H.; Powers, H.J. Sensitivity of markers of DNA stability and DNA repair activity to folate supplementation in healthy volunteers. Br. J. Cancer 2006, 94, 1942-1947.
39. Manach, C.; Hubert, J.; Llorach, R.; Scalbert, A. The complex links between dietary phytochemicals and human health deciphered by metabolomics. Mol. Nutr. Food Res. 2009, 53, 1303-1315.