Urea, the most abundant component in urine, cross-reacts with a commercial 8-OH-dG ELISA kit and contributes to overestimation of urinary 8-OH-dG

Free Radical Biology and Medicine

Volumn 47, Issue 1, 2009, Pages 41-46

  Song, Ming Fen ^a   Li, Yun Shan ^a   Ootsuyama, Yuko ^a   Kasai, Hiroshi ^a   Kawai, Kazuaki ^a   Ohta, Masanori ^a   Eguchi, Yasumasa ^a   Yamato, Hiroshi ^a   Matsumoto, Yuki ^b,c   Yoshida, Rie ^b   Ogawa, Yasutaka ^b,c  

^a UNIVERSITY OF OCCUPATIONAL AND ENVIRONMENTAL HEALTH   (Japan)

^b NATIONAL INSTITUTE OF OCCUPATIONAL SAFETY AND HEALTH   (Japan)

^c KITASATO UNIVERSITY   (Japan)

Author keywords

8 OH dG; Biomarker; ELISA; Free radicals; Oxidative stress

Indexed keywords

8 OXO 7,8 DIHYDRO 2 DEOXYGUANOSINE; DEOXYGUANOSINE; UNCLASSIFIED DRUG; UREA; UREASE;

ADULT; ARTICLE; CROSS REACTION; ENZYME LINKED IMMUNOSORBENT ASSAY; FRACTIONATION; HUMAN; MALE; OXIDATIVE STRESS; PRIORITY JOURNAL; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; URINALYSIS; URINE;

ADULT; BIOLOGICAL MARKERS; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CROSS REACTIONS; DEOXYGUANOSINE; DIAGNOSTIC ERRORS; ENZYME-LINKED IMMUNOSORBENT ASSAY; FEMALE; HUMANS; MALE; MIDDLE AGED; OXIDATIVE STRESS; REAGENT KITS, DIAGNOSTIC; TEMPERATURE; UREA;

MUS; RATTUS;

EID: 67349190916     PISSN: 08915849     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.freeradbiomed.2009.02.017     Document Type: Article

References (22)

1. Kasai H., Kawai K., and Li Y.-S. Analysis of 8-OH-dG and 8-OH-Gua as biomarkers of oxidative stress. Genes Environ. 30 (2008) 33-40
2. Cooke M.S., Evans M.D., Herbert K.E., and Lunec J. Urinary 8-oxo-2′-deoxyguanosine-source, significance and supplements. Free Radic. Res. 32 (2000) 381-397
3. Kasai H., Hayami H., Yamaizumi Z., Saito H., and Nishimura S. Detection and identification of mutagens and carcinogens as their adducts with guanosine derivatives. Nucleic Acids Res 12 (1984) 2127-2136
4. Floyd R.A., Watson J.J., Wong P.K., Altmiller D.H., and Rickard R.C. Hydroxyl free radical adduct of deoxyguanosine: sensitive detection and mechanisms of formation. Free Radic. Res. Commun. 1 (1986) 163-172
5. Kasai H. Analysis of a form of oxidative DNA damage, 8-hydroxy-2′-deoxyguanosine, as a marker of cellular oxidative stress during carcinogenesis. Mutat. Res 387 (1997) 147-163
6. Shigenaga M.K., Gimeno C.J., and Ames B.N. Urinary 8-hydroxy-2′-deoxyguanosine as a biological marker of in vivo oxidative DNA damage. Proc. Natl. Acad. Sci. U. S. A. 86 (1989) 9697-9701
7. Toyokuni S., Tanaka T., Hattori Y., Nishiyama Y., Yoshida A., Uchida K., Hiai H., Ochi H., and Osawa T. Quantitative immunohistochemical determination of 8-hydroxy-2′-deoxyguanosine by a monoclonal antibody N45.1: its application to ferric nitrilotriacetate-induced renal carcinogenesis model. Lab. Invest 76 (1997) 365-374
8. Yoshida R., Ogawa Y., and Kasai H. Urinary 8-oxo-7,8-dihydro-2′-deoxy-guanosine values measured by an ELISA correlated well with measurements by high-performance liquid chromatography with electrochemical detection. Cancer Epidemiol. Biomarkers Prev. 11 (2002) 1076-1081
9. Ravanat J.L., Duretz B., Guiller A., Douki T., and Cadet J. Isotope dilution high-performance liquid chromatography-electrospray tandem mass spectrometry assay for the measurement of 8-oxo-7,8-dihydro-2′-deoxyguanosine in biological samples. J. Chromatogr. B Biomed. Sci. Appl 18 (1998) 349-356
10. Shimoi K., Kasai H., Yokota N., Toyokuni S., and Kinae N. Comparison between high-performance liquid chromatography and enzyme-linked immunosorbent assay for the determination of 8-hydroxy-2′-deoxyguanosine in human urine. Cancer Epidemiol. Biomarkers Prev. 11 (2002) 767-770
11. Cooke M.S., Singh R., Hall G.K., Mistry V., Duarte T.L., Farmer P.B., and Evans M.D. Evaluation of enzyme-linked immunosorbent assay and liquid chromatography-tandem mass spectrometry methodology for the analysis of 8-oxo-7,8-dihydro-2′-deoxyguanosine in saliva and urine. Free Radic. Biol. Med. 41 (2006) 1829-1836
12. Cheng K.C., Cahill D.S., Kasai H., and Nishimura S. Loeb, L.A., 8-Hydroxyguanine, an abundant form of oxidative DNA damage, causes G→T and A→C substitutions. J. Biol. Chem 267 (1992) 166-172
13. Kasai H. A new automated method to analyze urinary 8-hydroxydeoxyguanosine by a high-performance liquid chromatography-electrochemical detector system. J. Radiat. Res 44 (2003) 185-189
14. Evans M.D., Singh R., Mistry V., Sandhu K., Farmer P.B., and Cooke M.S. Analysis of urinary 8-oxo-7,8-dihydro-purine-2′-deoxyribonucleosides by LC-MS/MS and improved ELISA. Free Radic. Res. 42 (2008) 831-840
15. Orita Y., Shirai D., Ando A., Takamitsu Y., Urakabe S., Furukawa T., and Abe H. Effect of urinary urea/nonurea on urinary concentrating ability and renal water economy in patients with renal diseases. Jpn. Circ. J. 31 (1967) 1299-1306
16. Yang B., and Bankir L. Urea and urine concentrating ability: new insights from studies in mice. Am. J. Physiol. Renal. Physiol. 288 (2005) F881-F896
17. Witherell H.L., Hiatt R.A., Replogle M., and Parsonnet J. Helicobacter pylori infection and urinary excretion of 8-hydroxy-2-deoxyguanosine, an oxidative DNA adduct. Cancer Epidemiol. Biomarkers Prev. 7 (1998) 91-96
18. Hu C.W., Wu M.T., Chao M.R., Pan C.H., Wang C.J., Swenberg J.A., and Wu K.Y. Comparison of analyses of urinary 8-hydroxy-2′-deoxyguanosine by isotope-dilution liquid chromatography with electrospray tandem mass spectrometry and by enzyme-linked immunosorbent assay. Rapid Commun. Mass Spectrom. 18 (2004) 505-510
19. Cooke M.S., Olinski R., Loft S., and members of ESCULA. Measurement and meaning of oxidatively modified DNA lesions in urine. Cancer Epidemiol. Biomarkers Prev. 17 (2008) 3-14
20. Appolonova S.A., Dikunets M.A., and Rodchenkov G.M. Possible indirect detection of rHuEPO administration in human urine by high-performance liquid chromatography tandem mass spectrometry. Eur. J. Mass Spectrom. 14 (2008) 201-209
21. Patel P.R., Bevan R.J., Mistry N., and Lunec J. Evidence of oligonucleotides containing 8-hydroxy-2′-deoxyguanosine in human urine. Free Radic. Biol. Med. 42 (2007) 552-558
22. Weimann A., Belling D., and Poulsen H.E. Quantification of 8-oxo-guanine and guanine as the nucleobase, nucleoside and deoxynucleoside forms in human urine by high-performance liquid chromatography-electrospray tandem mass spectrometry. Nucleic Acids Res. 30 (2002) E7