Improving the affinity of an antibody for its antigen via long-range electrostatic interactions

Protein Engineering, Design and Selection

Volumn 26, Issue 12, 2013, Pages 773-780

  Fukunaga, Atsushi ^a,b   Tsumoto, Kouhei ^a  

^a UNIVERSITY OF TOKYO   (Japan)

^b KYUSHU UNIVERSITY   (Japan)

Author keywords

Electrostatic interaction; Refolding; ScFv; SPR; Troponin

Indexed keywords

DISSOCIATION RATE CONSTANT; ISO-ELECTRIC POINTS; ORDERS OF MAGNITUDE; REFOLDING; SCFV; SINGLE-CHAIN ANTIBODIES; SPR; TROPONIN;

CHAINS; COULOMB INTERACTIONS; DISSOCIATION; ELECTROSTATIC SEPARATORS; EPITOPES; RATE CONSTANTS; SURFACE PLASMON RESONANCE;

ANTIBODIES;

EPITOPE; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY; TROPONIN I; RECOMBINANT PROTEIN;

ANTIBODY AFFINITY; ANTIGEN BINDING; ARTICLE; DISSOCIATION CONSTANT; ISOELECTRIC POINT; LONG RANGE ELECTROSTATIC FORCE; MOLECULAR STABILITY; MUTATION; PRIORITY JOURNAL; STATIC ELECTRICITY; SURFACE PLASMON RESONANCE; AMINO ACID SEQUENCE; BINDING SITE; CHEMISTRY; DIRECTED MOLECULAR EVOLUTION; GENETICS; HUMAN; KINETICS; METABOLISM; MOLECULAR GENETICS; OSMOLARITY; PROCEDURES; PROTEIN ENGINEERING; PROTEIN REFOLDING;

AMINO ACID SEQUENCE; ANTIBODY AFFINITY; BINDING SITES; DIRECTED MOLECULAR EVOLUTION; HUMANS; KINETICS; MOLECULAR SEQUENCE DATA; MUTATION; OSMOLAR CONCENTRATION; PROTEIN ENGINEERING; PROTEIN REFOLDING; RECOMBINANT PROTEINS; SINGLE-CHAIN ANTIBODIES; STATIC ELECTRICITY; SURFACE PLASMON RESONANCE; TROPONIN I;

EID: 84890506396     PISSN: 17410126     EISSN: 17410134     Source Type: Journal    
DOI: 10.1093/protein/gzt053     Document Type: Article

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