Stability engineering of scFvs for the development of bispecific and multivalent antibodies

Protein Engineering, Design and Selection

Volumn 23, Issue 7, 2010, Pages 549-557

  Miller, Brian R ^a   Demarest, Stephen J ^a   Lugovskoy, Alexey ^b   Huang, Flora ^a   Wu, Xiufeng ^a   Snyder, William B ^a   Croner, Lisa J ^c   Wang, Norman ^d   Amatucci, Aldo ^b   Michaelson, Jennifer S ^b   Glaser, Scott M ^a  

^a BIOGEN   (United States)

^b Medical Research   (United Kingdom)

^c Biosite Incorporated   (United States)

^d UNIVERSITY OF HAWAII   (United States)

Author keywords

antibody therapeutics; bispecific antibodies; protein engineering; protein stability; scFv

Indexed keywords

ANTIBODY THERAPEUTICS; BISPECIFIC ANTIBODIES; PROTEIN ENGINEERING; PROTEIN STABILITY; SCFV;

ANTIGENS; BINDING ENERGY; BIOCHEMICAL ENGINEERING; ESCHERICHIA COLI; GENETIC ENGINEERING; KNOWLEDGE BASED SYSTEMS; LIBRARIES; THERMODYNAMIC STABILITY;

ANTIBODIES;

ANTIBODY; BISPECIFIC ANTIBODY; DEATH RECEPTOR 5; IMMUNOGLOBULIN G; OLIGONUCLEOTIDE; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY;

ANTIBODY LIBRARY; ANTIBODY PRODUCTION; ANTIGEN BINDING; ARTICLE; BINDING AFFINITY; BIOENGINEERING; CONTROLLED STUDY; ESCHERICHIA COLI; HUMAN; HUMAN CELL; MUTATIONAL ANALYSIS; PRIORITY JOURNAL; SCREENING; SEQUENCE ANALYSIS; STRUCTURE ANALYSIS; TEMPERATURE SENSITIVITY; THERMOSTABILITY;

ANTIBODIES, BISPECIFIC; CHROMATOGRAPHY, GEL; ESCHERICHIA COLI; GENE LIBRARY; IMMUNOGLOBULIN G; LYMPHOTOXIN BETA RECEPTOR; MUTATION; PROTEIN ENGINEERING; PROTEIN STABILITY; RECEPTORS, TNF-RELATED APOPTOSIS-INDUCING LIGAND; SINGLE-CHAIN ANTIBODIES; TEMPERATURE;

EID: 77955976111     PISSN: 17410126     EISSN: 17410134     Source Type: Journal    
DOI: 10.1093/protein/gzq028     Document Type: Article

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