Identification of auxotrophic mutants of the yeast Kluyveromyces marxianus by non-homologous end joining-mediated integrative transformation with genes from Saccharomyces cerevisiae

Yeast

Volumn 30, Issue 12, 2013, Pages 485-500

  Yarimizu, Tohru ^a   Nonklang, Sanom ^b   Nakamura, Junpei ^a   Tokuda, Shuya ^a   Nakagawa, Takaaki ^a   Lorreungsil, Sasithorn ^b   Sutthikhumpha, Surasit ^b   Pukahuta, Charida ^b   Kitagawa, Takao ^a   Nakamura, Mikiko ^c   Cha aim, Kamonchai ^d   Limtong, Savitree ^e   Hoshida, Hisashi ^a   Akada, Rinji ^a  

^a YAMAGUCHI UNIVERSITY GRADUATE SCHOOL OF MEDICINE   (Japan)

^b UBON RATCHATHANI UNIVERSITY   (Thailand)

^c YAMAGUCHI UNIVERSITY   (Japan)

^d SRINAKHARINWIROT UNIVERSITY   (Thailand)

^e KASETSART UNIVERSITY   (Thailand)

Author keywords

Auxotrophic mutants; Kluyveromyces marxianus; Mating; Non homologous end joining; Saccharomyces cerevisiae; Tetrad

Indexed keywords

DNA FRAGMENT;

AMINO ACID METABOLISM; ARTICLE; AUXOTROPHIC MUTANT; CHROMOSOME; DNA END JOINING REPAIR; FUNGAL STRAIN; GENE AMPLIFICATION; GENE LIBRARY; GENE MUTATION; GENE SYNTHESIS; GENETIC CODE; GENETIC COMPLEMENTATION; GENETIC IDENTIFICATION; GENETIC TRANSFORMATION; KLUYVEROMYCES MARXIANUS; MATING; MUTATION RATE; NONHUMAN; NUCLEIC ACID METABOLISM; NUTRITIONAL REQUIREMENT; PHENOTYPE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SACCHAROMYCES CEREVISIAE; SPOROGENESIS;

KLUYVEROMYCES MARXIANUS; SACCHAROMYCES CEREVISIAE;

AUXOTROPHIC MUTANTS; KLUYVEROMYCES MARXIANUS; MATING; NON-HOMOLOGOUS END-JOINING; SACCHAROMYCES CEREVISIAE; TETRAD;

DNA, FUNGAL; GENETIC COMPLEMENTATION TEST; KLUYVEROMYCES; MUTATION; PLASMIDS; RECOMBINATION, GENETIC; SACCHAROMYCES CEREVISIAE; TRANSFORMATION, GENETIC; TRANSGENES;

EID: 84890183384     PISSN: 0749503X     EISSN: 10970061     Source Type: Journal    
DOI: 10.1002/yea.2985     Document Type: Article

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