In vivo investigation of cardiac metabolism in the rat using MRS of hyperpolarized [1-13C] and [2-13C]pyruvate

NMR in Biomedicine

Volumn 26, Issue 12, 2013, Pages 1680-1687

  Josan, Sonal ^a,b   Park, Jae Mo ^b,c   Hurd, Ralph ^d   Yen, Yi Fen ^b   Pfefferbaum, Adolf ^a,e   Spielman, Daniel ^b,c   Mayer, Dirk ^a,b  

^a SRI INTERNATIONAL   (United States)

^b STANFORD UNIVERSITY   (United States)

^c Stanford University   (United States)

^d GE HEALTHCARE   (United States)

^e STANFORD UNIVERSITY   (United States)

Author keywords

Dichloroacetate; Dobutamine; Dose; Heart; Hyperpolarized 13C; Metabolism; Pyruvate

Indexed keywords

DICHLOROACETATE; DOBUTAMINE; DOSE; HYPERPOLARIZED; PYRUVATES;

AMINO ACIDS; CARBON DIOXIDE; DICHLOROACETIC ACID; HEART; PHYSIOLOGY;

METABOLISM;

1 ACETYLCARNITINE C 13; 1,3 ACETOACETATE C 13; 2 ALANINE C 13; 2 LACTATE C 13; 2 PYRUVATE C 13; 5 GLUTAMATE C 13; ACETOACETIC ACID; ACETYL COENZYME A; ACETYLCARNITINE; ALANINE; ALANINE C 13; DOBUTAMINE; GLUTAMIC ACID; LACTIC ACID; PYRUVIC ACID; PYRUVIC ACID C 13; RADIOPHARMACEUTICAL AGENT; UNCLASSIFIED DRUG;

ANIMAL EXPERIMENT; ARTICLE; HEART; HEART MUSCLE METABOLISM; HEART MUSCLE OXYGEN CONSUMPTION; HEART RATE; IN VIVO STUDY; MALE; NONHUMAN; NUCLEAR MAGNETIC RESONANCE; OXIDATION; PRIORITY JOURNAL; RAT;

RATTUS;

DICHLOROACETATE; DOBUTAMINE; DOSE; HEART; HYPERPOLARIZED 13C; METABOLISM; PYRUVATE;

ANIMALS; CARBON ISOTOPES; DICHLOROACETIC ACID; DOBUTAMINE; MAGNETIC RESONANCE SPECTROSCOPY; MALE; MYOCARDIUM; PYRUVIC ACID; RATS; RATS, WISTAR; TIME FACTORS;

EID: 84888050726     PISSN: 09523480     EISSN: 10991492     Source Type: Journal    
DOI: 10.1002/nbm.3003     Document Type: Article

References (34)

1. Kurhanewicz J, Vigneron DB, Brindle K, Chekmenev EY, Comment A, Cunningham CH, Deberardinis RJ, Green GG, Leach MO, Rajan SS, Rizi RR, Ross BD, Warren WS, Malloy CR. Analysis of cancer metabolism by imaging hyperpolarized nuclei: prospects for translation to clinical research. Neoplasia 2011; 13: 81-97.
2. 13C magnetic resonance spectroscopy. Magn. Reson. Med. 2011; 66: 505-519.
3. 13C MRI. Magn. Reson. Med. 2008; 59: 1005-1013.
4. Bhattacharya P, Ross BD, Bünger R. Cardiovascular applications of hyperpolarized contrast media and metabolic tracers. Exp. Biol. Med. 2009; 234(12): 1395-1416.
5. 13C MRI assist clinical decision-making for patients with heart disease? NMR Biomed. 2011; 24: 973-979.
6. Schroeder MA, Clarke K, Neubauer S, Tyler DJ. Hyperpolarized magnetic resonance: a novel technique for the in vivo assessment of cardiovascular disease. Circulation 2011; 124: 1580-1594.
7. 13C allows a direct measure of flux through a single enzyme-catalyzed step by NMR. Proc. Natl Acad. Sci. USA 2007; 104(50): 19773-19777.
8. Schroeder MA, Cochlin LE, Heather LC, Clarke K, Radda GK, Tyler DJ. In vivo assessment of pyruvate dehydrogenase flux in the heart using hyperpolarized carbon-13 magnetic resonance. Proc. Natl Acad. Sci. USA 2008; 105(33): 12051-12056.
9. 13C pyruvate and bicarbonate in the heart. Magn. Reson. Med. 2010; 64: 1323-1331.
10. Atherton HJ, Dodd MS, Heather LC, Schroeder MA, Griffin JL, Radda GK, Clarke K, Tyler D. Role of pyruvate dehydrogenase inhibition in the development of hypertrophy in the hyperthyroid rat heart: a combined magnetic resonance imaging and hyperpolarized magnetic resonance spectroscopy study. Circulation 2011; 123: 2552-2561.
11. Dodd MS, Ball DR, Schroeder MA, Le Page LM, Atherton HJ, Heather LC, Seymour A-M, Ashrafian H, Watkins H, Clarke K, Tyler D. In vivo alterations in cardiac metabolism and function in the spontaneously hypertensive rat heart. Cardiovasc. Res. 2012; 95: 69-76.
12. 13C MRS. NMR Biomed. 2010; 24(2): 201-208.
13. 13C] lactate for the in vivo investigation of cardiac metabolism. NMR Biomed. 2012. DOI: 10.1002/nbm.2778
14. 13C magnetic resonance spectroscopy. FASEB J. 2009; 23(8): 2529-2538.
15. 2]pyruvate in vivo. NMR Biomed. 2011. DOI: 10.1002/nbm.1749
16. Schroeder MA, Atherton HJ, Dodd MS, Lee P, Cochlin LE, Radda GK, Clarke K, Tyler D. The cycling of acetyl-coenzyme A through acetylcarnitine buffers cardiac substrate supply. Circulation: Cardiovasc. Imaging 2012. DOI: 10.1161/circimaging.111
17. 13C]pyruvate to probe the effects of the anticancer agent dichloroacetate on mitochondrial metabolism in vivo in the normal rat. Magn Reson Imaging 2012; 30: 1367-1372.
18. 13C]pyruvate. Am. J. Physiol. Heart Circ. Physiol. 2010; 298(5): H1556-1564.
19. Schroeder MA, Atherton HJ, Cochlin LE, Clarke K, Radda GK, Tyler DJ. The effect of hyperpolarized tracer concentration on myocardial uptake and metabolism. Magn. Reson. Med. 2009; 61: 1007-1014. DOI: 10.1002/mrm.21934
20. 13C]pyruvate in pigs using slice selective magnetic resonance spectroscopy. Contrast Media Mol. Imaging 2012; 7: 85-94.
21. Stanley WC. Myocardial substrate metabolism in the normal and failing heart. Physiolog. Rev. 2005; 85: 1093-1129.
22. Stacpoole PW. The pharmacology of dichloroacetate. Metabolism 1989; 38: 1124-1144.
23. Sharma N. Regulation of pyruvate dehydrogenase activity and citric acid cycle intermediates during high cardiac power generation. J. Physiol. 2005; 562: 593-603.
24. LaNoue KF, Walajtys EI, Williamson JR. Regulation of glutamate metabolism and interactions with the citric acid cycle in rat heart mitochondria. J. Biol. Chem. 1973; 248: 7171-7183.
25. Williamson JR. Mitochondrial function in the heart. Annu. Rev. Physiol. 1979; 41: 485-506.
26. ODonnell JM, Kudej RK, LaNoue KF, Vatner SF, Lewandowski ED. Limited transfer of cytosolic NADH into mitochondria at high cardiac workload. Am. J. Physiol. Heart Circ. Physiol. 2004; 286: H2237-2242.
27. Neely J, Denton RM, England PJ, Randle PJ. The effects of increased heart work on the tricarboxylate cycle and its interactions with glycolysis in the perfused rat heart. Biochem. J. 1972; 128: 147-159.
28. Angelos MG, Torres, CAA, Rath DP, Zhu H, Beckely PD, Robitaille P-M. In-vivo myocardial substrate alteration during perfused ventricular fibrillation. Acad. Emerg. Med. 1999; 6: 581-587.
29. Wilson DM, Keshari KR, Larson PEZ, Chen AP, Hu S, Van Criekinge M, Bok R, Nelson SJ, Macdonald JM, Vigneron DB, Kurhanewicz J. Multi-compound polarization by DNP allows simultaneous assessment of multiple enzymatic activities in vivo. J. Magn. Reson. 2010; 205: 141-147.
30. McGarry JD, Foster DW. Ketogenesis and cholesterol synthesis in normal and neoplastic tissues of the rat. J. Biol. Chem. 1969; 244: 4251-4256.
31. Krebs HA. Rate control of the tricarboxylic acid cycle. Adv. Enzyme Regul. 1970; 8: 335-353.
32. Opie LH, Owen P. Effects of increased mechanical work by isolated perfused rat heart during production or uptake of ketone bodies. Biochem. J. 1975; 148: 403-415.
33. 13C]pyruvate in vivo using a sub-kelvin, multi-sample DNP polarizer. Magn. Reson. Imaging 2012. DOI: 10.1016/j.mri.2012.09.002
34. 13C-pyruvate. Magn. Reson. Med. 2009; 62: 1-10.