Hyperpolarized 13C allows a direct measure of flux through a single enzyme-catalyzed step by NMR

Proceedings of the National Academy of Sciences of the United States of America

Volumn 104, Issue 50, 2007, Pages 19773-19777

  Merritt, Matthew E ^a,b   Harrison, Crystal ^a,d   Storey, Charles ^a   Jeffrey, F Mark ^a,b   Sherry, A Dean ^a,b,d   Malloy, Craig R ^a,b,c  

^a UNIVERSITY OF TEXAS SOUTHWESTERN MEDICAL CENTER   (United States)

^b UNIVERSITY OF TEXAS SOUTHWESTERN MEDICAL CENTER   (United States)

^c VA NORTH TEXAS HEALTH CARE SYSTEM   (United States)

^d UNIVERSITY OF TEXAS AT DALLAS   (United States)

Author keywords

Carbon dioxide; Heart; Hyperpolarization; NMR spectroscopy; Pyruvate

Indexed keywords

CARBON 13; CARBON DIOXIDE; GLUTAMIC ACID; LACTIC ACID; OCTANOIC ACID; PYRUVATE DEHYDROGENASE; PYRUVIC ACID; CARBON; ENZYME;

ANALYTICAL EQUIPMENT; ANIMAL TISSUE; ARTICLE; CARBON NUCLEAR MAGNETIC RESONANCE; CATALYSIS; CITRIC ACID CYCLE; ENZYME ACTIVITY; HEART MUSCLE METABOLISM; HYPERPOLARIZATION; ISOLATED HEART; ISOTOPE LABELING; NONHUMAN; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; OXYGEN CONSUMPTION; PRIORITY JOURNAL; RAT; ANIMAL; CHEMISTRY; ENZYMOLOGY; HEART MUSCLE; MALE; METHODOLOGY;

RATTUS;

ANIMALS; CARBON ISOTOPES; CATALYSIS; ENZYMES; MAGNETIC RESONANCE SPECTROSCOPY; MALE; MYOCARDIUM; RATS;

EID: 38049103113     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.0706235104     Document Type: Article

References (32)

1. Shulman RG, Rothman DL, eds (2005) Metabolomics by in Vivo NMR (Wiley, Chichester, UK).
2. Ardenkjaer-Larsen JH, Fridlund B, Gram A, Hansson G, Hansson L, Lerche MH, Servin R, Thaning M, Golman K (2003) Proc Natl Acad Sci USA 100:10158-10163.
3. Golman K, Petersson JS (2006) Acad Radiol 13:932-942.
4. Jeffrey FMH, Malloy CR (1992) Biochem J 287:117-123.
5. Taegtmeyer H (1984) Basic Res Cardiol 79:322-336.
6. Chaitman BR, Skettino SL, Parker JO, Hanley P, Meluzin J, Kuch J, Pepine CJ, Wang W, Nelson JJ, Hebert DA, et al. (2004) J Am Coll Cardiol 43:1375-1382.
7. Opie LH (1970) Nature 227:1055-1056.
8. Sodi-Pallares D, Testelli MR, Fishleder BI, Bisteni A, Medrano GA, Friedland C, De Micheli A (1962) Am J Cardiol 9:166-181.
9. Vik-Mo H, Mjos OD (1981) Am J Cardiol 48:361-365.
10. Lee L, Campbell R, Scheuermann-Freestone M, Taylor R, Gunaruwan P, Williams L, Ashrafian H, Horowitz J, Fraser AG, Clarke K, et al. (2005) Circulation 112:3280-3288.
11. Neubauer S (2007) N Engl J Med 356:1140-1151.
12. Mehta SR, Yusuf S, Diaz R, Zhu J, Pais P, Xavier D, Paolasso E, Ahmed R, Xie C, Kazmi K, et al. (2005) J Am Med Assoc 293:437-446.
13. Latipaa PM, Peuhkurinen KJ, Hiltunen JK, Hassinen IE (1985) J Mol Cell Cardiol 17:1161-1171.
14. Dennis SC, Padma A, DeBuysere MS, Olson MS (1979) J Biol Chem 254:1252-1258.
15. Olson MS, Dennis SC, DeBuysere MS, Padma A (1978) J Biol Chem 253:7369-7375.
16. Day SE, Gallagher FA, Kettunen MI, Brindle KM (2007) Proc Int Soc Magn Reson Med (Int Soc for Magn Reson in Med, Berlin, Germany), p 368.
17. Malloy CR, Sherry AD, Jeffrey FMH (1988) J Biol Chem 263:6964-6971.
18. Malloy CR, Sherry AD, Jeffrey FMH (1990) Am J Physiol 259:H987-H995.
19. Lindskog S, Henderson L, Kannan K, Liljas A, Nyman P, Strandberg B (1971) in The Enzymes, ed Boyer PD (Academic Press, New York), Vol 5, pp 587-665.
20. Bailey IA, Gadian DG, Matthews PM, Radda GK, Seeley PJ (1981) FEBS Lett 123:315-318.
21. Malloy CR, Sherry AD, Jeffrey FMH (1987) FEBS Lett 212:58-62.
22. Sherry AD, Nunnally RL, Peshock RM (1985) J Biol Chem 260:9272-9279.
23. Ardenkjaer-Larsen JH, Fridlund B, Gram A, Hansson G, Hansson L, Lerche MH, Servin R, Thaning M, Golman K (2003) Proc Natl Acad Sci USA 100:10158-10163.
24. Golman K, in't Zandt R, Thaning M (2006) Proc Natl Acad Sci USA 103:11270-11275.
25. Taegtmeyer H (1983) Basic Res Cardiol 78:435-450.
26. Gout E, Bligny R, Douce R (1992) J Biol Chem 267:13903-13909.
27. Garlick PB, Radda GK, Seeley PJ (1979) J Biochem 184:547-554.
28. Golman K, Ardenkjaer-Larsen JH, Petersson JS, Mansson S, Leunbach I (2003) Proc Natl Acad Sci USA 100:10435-10439.
29. Liger-Belair G, Prost E, Parmentier M, Jeandet P, Nuzillard JM (2003) J Agric Food Chem 51:7560-7563.
30. Shporer M, Forster RE, Civan MM (1984) Am J Physiol 246:C231-C234.
31. Malloy CR, Sherry AD, Nunnally RL (1985) J Magn Reson 64:243-254.
32. Malloy CR, Jones JG, Jeffrey FMH, Jessen ME, Sherry AD (1996) MAGMA 4:35-46.