Live single cell functional phenotyping in droplet nano-liter reactors

Scientific Reports

Volumn 3, Issue , 2013, Pages

  Konry, Tania ^a   Golberg, Alexander ^b   Yarmush, Martin ^b  

^a NORTHEASTERN UNIVERSITY   (United States)

^b MASSACHUSETTS GENERAL HOSPITAL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CD86 ANTIGEN; INTERLEUKIN 6;

ANIMAL; BONE MARROW CELL; C57BL MOUSE; CYTOLOGY; DENDRITIC CELL; DEVICES; FEMALE; IMMUNOLOGY; IMMUNOPHENOTYPING; METABOLISM; MICROFLUIDIC ANALYSIS; MOUSE; NANOTECHNOLOGY; PROCEDURES; T LYMPHOCYTE;

ANIMALS; ANTIGENS, CD86; BONE MARROW CELLS; DENDRITIC CELLS; FEMALE; IMMUNOPHENOTYPING; INTERLEUKIN-6; MICE; MICE, INBRED C57BL; MICROFLUIDIC ANALYTICAL TECHNIQUES; NANOTECHNOLOGY; T-LYMPHOCYTES;

EID: 84887847432     PISSN: None     EISSN: 20452322     Source Type: Journal    
DOI: 10.1038/srep03179     Document Type: Article

References (27)

1. Bordbar, A. et al. Model-driven multi-omic data analysis elucidates metabolic immunomodulators of macrophage activation. Mol. Syst. Biol. 8, 1-12 (2012).
2. Katayama, H. et al. Phenotype and functional identity of GM-CSF-independent dendritic cells generated by long-term propagation of DC progenitor cells in bone marrow cells and skin langerhans cells. Transplant. Proc. 37, 17-19 (2005). (Pubitemid 40459674)
3. Nielsen, J. & Oliver, S. The next wave in metabolome analysis. Trends Biotechnol 23, 544-546 (2005). (Pubitemid 41483770)
4. Sul, J.-Y. et al. Transcriptome transfer produces a predictable cellular phenotype. Proc. Natl. Acad. Sci. USA (2009).
5. Fritzsch, F. S. O., Dusny, C., Frick, O. & Schmid, A. Single-Cell Analysis in Biotechnology, Systems Biology, and Biocatalysis. Annu. Rev. Chem. Biomol. Eng. 3, 129-155 (2012).
6. Ghim, C.-M. & Almaas, E. Genetic noise control via protein oligomerization. BMC Syst. Biol. 2, 94 (2008).
7. Kuang, Y., Biran, I. & Walt, D. R. Simultaneously Monitoring Gene Expression Kinetics and Genetic Noise in Single Cells by Optical Well Arrays. Anal. Chem. 76, 6282-6286 (2004). (Pubitemid 39458460)
8. Han, Q. et al. Polyfunctional responses by human T cells result from sequential release of cytokines. Proc Natl Acad Sci U S A 109, 1607-1612 (2012).
9. Lu, Y. et al. High-throughput secretomic analysis of single cells to assess functional cellular heterogeneity. Anal Chem 85, 2548-2556 (2013).
10. Varadarajan, N. et al. A high-throughput single-cell analysis of human CD8(1) T cell functions reveals discordance for cytokine secretion and cytolysis. J Clin Invest 121, 4322-4331 (2010).
11. Willmann, K. & Dunne, J. F. A flow cytometric immune function assay for human peripheral blood dendritic cells. J. Leukocyte Biol. 67, 536-544 (2000).
12. Xu, Y., Zhan, Y., Lew, A. M., Naik, S. H. & Kershaw, M. H. Differential Development of Murine Dendritic Cells by GM-CSF versus Flt3 Ligand Has Implications for Inflammation and Trafficking. J Immunol 179, 7577-7584 (2007).
13. Decker, P., Kotter, I., Klein, R., Berner, B. & Rammensee, H.-G.Monocyte-derived dendritic cells over-express CD86 in patients with systemic lupus erythematosus. Rheumatology 45, 1087-1095 (2006). (Pubitemid 44542093)
14. Geisel, J. et al. IL-6 and Maturation Govern TLR2 and TLR4 Induced TLR Agonist Tolerance and Cross-Tolerance in Dendritic Cells. The Journal of Immunology 179, 5811-5818 (2007).
15. Granucci, F. et al. Early events in dendritic cell maturation induced by LPS. Microbes Infect. 1, 1079-1084 (1999). (Pubitemid 29496089)
16. Hegde, S., Pahne, J. & Smola-Hess, S. Novel immunosuppressive properties of interleukin-6 in dendritic cells: inhibition of NF-kappaB binding activity and CCR7 expression. The FASEB Journal (2004).
17. Jiang, H.-R. et al. Secretion of interleukin-10 or interleukin-12 by LPS-activated dendritic cells is critically dependent on time of stimulus relative to initiation of purified DC culture. J. Leukocyte Biol. 72, 978-985 (2002). (Pubitemid 35365172)
18. Park, S.-J. et al. IL-6 Regulates In Vivo Dendritic Cell Differentiation through STAT3 Activation. J Immunol 173, 3844-3854 (2004). (Pubitemid 39280747)
19. Chemin, K. et al. Cytokine secretion by CD41 T cells at the immunological synapse requires Cdc42-dependent local actin remodeling but not microtubule organizing center polarity. J Immunol 189, 2159-2168 (2012).
20. Duchez, S., Rodrigues, M., Bertrand, F. & Valitutti, S. Reciprocal polarization of T and B cells at the immunological synapse. J Immunol 187, 4571-4580 (2011).
21. Einav, S. et al. Discovery of a hepatitis C target and its pharmacological inhibitors by microfluidic affinity analysis. Nat Biotechnol 26, 1019-1027 (2008).
22. Gunzer, M. et al. Antigen presentation in extracellular matrix: interactions of T cells with dendritic cells are dynamic, short lived, and sequential. Immunity 13, 323-332 (2000).
23. Agresti, J. J. et al. Ultrahigh-throughput screening in drop-based microfluidics for directed evolution. Proc. Natl. Acad. Sci. USA 107, 4004-4009 (2010).
24. Kiss, M. M. et al. High-Throughput Quantitative Polymerase Chain Reaction in Picoliter Droplets. Anal Chem. 80, 8975-8981 (2008).
25. Konry, T., Dominguez-Villar, M., Baecher-Allan, C., Hafler, D. A. & Yarmush, M. L. Droplet-based microfluidic platforms for single T cell secretion analysis of IL-10 cytokine. Biosens. Bioelectron. 26, 2707-2710 (2011).
26. Konry, T., Smolina, I., Yarmush, J.M., Irimia, D. & Yarmush, M. L. Ultrasensitive Detection of Low-Abundance Surface-Marker Protein Using Isothermal Rolling Circle Amplification in a Microfluidic Nanoliter Platform. Small 7, 395-400 (2011).
27. Koster, S. et al. Drop-based microfluidic devices for encapsulation of single cells. Lab Chip 8, 1110-1115 (2008). (Pubitemid 351904829)