A flow cytometric immune function assay for human peripheral blood dendritic cells

Journal of Leukocyte Biology

Volumn 67, Issue 4, 2000, Pages 536-544

  Willmann, K ^a   Dunne, J F ^a  

^a NONE   (United States)

Author keywords

Cytokines; Ionomycin; Lipopolysaccharide; Phorbol myristate acetate

Indexed keywords

B7 ANTIGEN; CD40 ANTIGEN; CD86 ANTIGEN; CYTOKINE; HLA DQ ANTIGEN; HLA DR ANTIGEN; INTERLEUKIN 1 RECEPTOR BLOCKING AGENT; INTERLEUKIN 1BETA; INTERLEUKIN 2 RECEPTOR; INTERLEUKIN 6; INTERLEUKIN 8; TUMOR NECROSIS FACTOR ALPHA;

ARTICLE; BLOOD CELL; CELL ACTIVATION; CYTOKINE PRODUCTION; DENDRITIC CELL; FLOW CYTOMETRY; HUMAN; HUMAN CELL; IMMUNE FUNCTION TEST; IMMUNOPHENOTYPING; PRIORITY JOURNAL;

EID: 0033849699     PISSN: 07415400     EISSN: None     Source Type: Journal    
DOI: 10.1002/jlb.67.4.536     Document Type: Article

References (39)

1. Dendritic cells and the control of immunity
2. Dendritic cells: Unique leukocyte populations which control the primary immune response
3. Dendritic cell surface molecules
4. Characterization of CMRF-44, a novel monoclonal antibody to an activation antigen expressed by the allostimulatory cells within peripheral blood, including dendritic cells
5. Isolation of human blood dendritic cells using the CMRF-44 monoclonal antibody: Implications for studies on antigen-presenting cell function and immunotherapy
6. Human blood dendritic cells selectively express CD83, a member of the immunoglobulin superfamily
7. + dendritic cells
8. Dendritic cells freshly isolated from human blood express CD4 and mature into typical immunostimulatory dendritic cells after culture in monocyte-conditioned medium
9. Activation of human peripheral blood dendritic cells induces the CD86 co-stimulatory molecule
10. Antigen capture and major histocompatibility class II compartments of freshly isolated and cultured human blood dendritic cells
11. Human blood contains two subsets of dendritic cells, one immunologically mature and the other immature
12. The enigmatic plasmacytoid T cells develop into dendritic cells with interleukin (IL)-3 and CD40-ligand
13. Dendritic cells capable of stimulating T cells in germinal centres
14. Human peripheral blood dendritic cell subsets. Isolation and characterization of precursor and mature antigen-presenting cells
15. Dendritic cell ontogeny: A human dendritic cell lineage of myeloid origin
16. Cytokine expression by human peripheral blood dendritic cells stimulated in vitro with HIV-1 and Herpes Simplex Virus
17. + blood dendritic cells
18. Detection of antigen-specific T cell cytokine expression in whole blood by flow cytometry
19. + T cell frequencies by flow cytometry
20. Detection of intracellular cytokines by flow cytometry
21. Direct demonstration of cytokine synthesis heterogeneity among human memory/effector T cells by flow cytometry
22. Increased light scattering resolution facilitates multidimensiional flow cytometric analysis
23. + blood monocytes exhibits features of tissue macrophages
24. A novel dendritic cell population in human blood: One-step immunomagnetic isolation by a specific mAb (M-DC8) and in vitro priming of cytotoxic T lymphocytes
25. Brefeldin A: Insights into the control of membrane traffic and organelle structure
26. Shedding as a mechanism of down-modulation of CD14 on stimulated human monocytes
27. The cytokine profile expressed by human dendritic cells is dependent on cell subtype and mode of activation
28. Phenotypic and functional properties of dendritic cells isolated from human peripheral blood in comparison with mononuclear cells and T cells
29. Dendritic cells use macropinocytosis and the mannose receptor to concentrate macromolecules in the major histocompatibility complex class 11 compartment: Downregulation by cytokines and bacterial products
30. Immunocytochemical detection of cytokines and chemokines in Langerhans cells and in vitro derived dendritic cells
31. CD11c/CD18, a transmembrane signaling receptor for lipopolysaccharide
32. Phorbol diester receptor copurifies with protein kinase C
33. Protein kinase C and lipid signaling for sustained cellular responses
34. Protein kinase C in the transduction of signals toward and within the cell nucleus
35. A cell type-specific enhancer in the human B7.1 gene regulated by NF-κB
36. Nuclear factor kappa B, a mediator of lipopolysaccharide effects
37. Cytokine signal transduction
38. Interleukin 1 receptor antagonist (IL-1Ra) is an acute-phase protein
39. Human peripheral blood-derived dendritic cells do not produce interleukin 1α, interleukin 1β, or interleukin 6