Fully automated analysis of chemically induced γH2AX foci in human peripheral blood mononuclear cells by indirect immunofluorescence

Cytometry Part A

Volumn 83, Issue 11, 2013, Pages 1017-1026

  Willitzki, Annika ^a   Lorenz, Sebastian ^b   Hiemann, Rico ^c   Guttek, Karina ^a   Goihl, Alexander ^a   Hartig, Roland ^a   Conrad, Karsten ^d   Feist, Eugen ^e   Sack, Ulrich ^f   Schierack, Peter ^c   Heiserich, Lisa ^b   Eberle, Caroline ^b   Peters, Vanessa ^b   Roggenbuck, Dirk ^b,c   Reinhold, Dirk ^a  

^a OTTO VON GUERICKE UNIVERSITY   (Germany)

^b Medipan GmbH   (Germany)

^c BRANDENBURG UNIVERSITY OF TECHNOLOGY   (Germany)

^d DRESDEN UNIVERSITY OF TECHNOLOGY   (Germany)

^e CHARITÉ UNIVERSITÄTSMEDIZIN BERLIN   (Germany)

^f UNIVERSITY OF LEIPZIG   (Germany)

Author keywords

H2AX foci; Automated microscopy; DNA double strand breaks; Etoposide; Human PBMCs; Image analysis

Indexed keywords

ETOPOSIDE; H2AFX PROTEIN, HUMAN; HISTONE;

ARTICLE; AUTOMATED MICROSCOPY; BLOOD; DNA DAMAGE; DNA DOUBLE-STRAND BREAKS; DOUBLE STRANDED DNA BREAK; DRUG EFFECT; FLUORESCENT ANTIBODY TECHNIQUE; GENETICS; HUMAN; HUMAN PBMCS; IMAGE ANALYSIS; IMMUNOLOGY; ISOLATION AND PURIFICATION; MONONUCLEAR CELL; ΓH2AX FOCI;

AUTOMATED MICROSCOPY; DNA DOUBLE-STRAND BREAKS; ETOPOSIDE; HUMAN PBMCS; IMAGE ANALYSIS; ΓH2AX FOCI;

DNA BREAKS, DOUBLE-STRANDED; DNA DAMAGE; ETOPOSIDE; FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT; HISTONES; HUMANS; LEUKOCYTES, MONONUCLEAR;

EID: 84886402782     PISSN: 15524922     EISSN: 15524930     Source Type: Journal    
DOI: 10.1002/cyto.a.22350     Document Type: Article

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