Automated gamma-H2AX focus scoring method for human lymphocytes after ionizing radiation exposure

Radiation Measurements

Volumn 46, Issue 9, 2011, Pages 871-876

  Valente, M ^a   Voisin, P ^a   Laloi, P ^a   Roy, L ^a   Roch Lefvre, S ^a  

^a INSTITUT DE RADIOPROTECTION ET DE SÛRETÉ NUCLÉAIRE IRSN   (France)

Author keywords

Automated scoring; Gamma H2AX foci; Lymphocytes

Indexed keywords

AUTOMATED APPROACH; AUTOMATED SCORING; BLOOD SAMPLES; GAMMA-H2AX FOCI; HUMAN LYMPHOCYTES; HUMAN PERIPHERAL BLOOD; LINEAR CORRELATION; LYMPHOCYTES; MINIMUM OPERATOR; RADIATION SENSITIVITY; SCORING METHODS; SEMI-AUTOMATED;

AMINO ACIDS; BLOOD; GAMMA RAYS; IONIZING RADIATION; PHOSPHORYLATION;

AUTOMATION;

EID: 80052955031     PISSN: 13504487     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.radmeas.2011.05.012     Document Type: Conference Paper

References (15)

1. L. Beels, J. Werbrouck, and H. Thierens Dose response and repair kinetics of gamma-H2AX foci induced by in vitro irradiation of whole blood and T-lymphocytes with X- and gamma-radiation Int. J. Radiat. Biol. 86 2010 760 768
2. W. Bocker, and G. Iliakis Computational methods for analysis of foci: validation for radiation-induced gamma-H2AX foci in human cells Radiat. Res. 165 2006 113 124 (Pubitemid 43023331)
3. A.E. Carpenter, T.R. Jones, M.R. Lamprecht, C. Clarke, I.H. Kang, O. Friman, D.A. Guertin, J.H. Chang, and R.A. Lindquist CellProfiler: image analysis software for identifying and quantifying cell phenotypes Genome Biol. 7 2006 R100
4. S.V. Costes, A. Boissiere, S. Ravani, R. Romano, B. Parvin, and M.H. Barcellos-Hoff Imaging features that discriminate between foci induced by high- and low-LET radiation in human fibroblasts Radiat. Res. 165 2006 505 515
5. Y.N. Hou, A. Lavaf, D. Huang, S. Peters, R. Huq, V. Friedrich, B.S. Rosenstein, and J. Kao Development of an automated gamma-H2AX immunocytochemistry assay Radiat. Res. 171 2009 360 367
6. I.H. Ismail, T.I. Wadhra, and O. Hammarsten An optimized method for detecting gamma-H2AX in blood cells reveals a significant interindividual variation in the gamma-H2AX response among humans Nucleic Acids Res. 35 2007 e36
7. A.N. Ivashkevich, O.A. Martin, A.J. Smith, C.E. Redon, W.M. Bonner, R.F. Martin, and P.N. Lobachevsky gammaH2AX foci as a measure of DNA damage: a computational approach to automatic analysis Mutat. Res. 2011
8. G. Jost, S. Golfier, H. Pietsch, P. Lengsfeld, M. Voth, T.E. Schmid, F. Eckardt-Schupp, and E. Schmid The influence of x-ray contrast agents in computed tomography on the induction of dicentrics and gamma-H2AX foci in lymphocytes of human blood samples Phys. Med. Biol. 54 2009 6029 6039
9. A. Jucha, A. Wegierek-Ciuk, Z. Koza, H. Lisowska, A. Wojcik, M. Wojewodzka, and A. Lankoff FociCounter: a freely available PC programme for quantitative and qualitative analysis of gamma-H2AX foci Mutat. Res. 696 2010 16 20
10. E.L. Leatherbarrow, J.V. Harper, F.A. Cucinotta, and P. O'Neill Induction and quantification of gamma-H2AX foci following low and high LET-irradiation Int. J. Radiat. Biol. 82 2006 111 118
11. M. Mistrik, L. Oplustilova, J. Lukas, and J. Bartek Low-dose DNA damage and replication stress responses quantified by optimized automated single-cell image analysis Cell Cycle 8 2009 2592 2599
12. S. Roch-Lefvre, T. Mandina, P. Voisin, G. Gaetan, J.E. Mesa, M. Valente, P. Bonnesoeur, O. Garcia, P. Voisin, and L. Roy Quantification of gamma-H2AX foci in human lymphocytes: a method for biological dosimetry after ionizing radiation exposure Radiat. Res. 174 2010 185 194
13. E.P. Rogakou, D.R. Pilch, A.H. Orr, V.S. Ivanova, and W.M. Bonner DNA double-stranded breaks induce histone H2AX phosphorylation on serine 139 J. Biol. Chem. 273 1998 5858 5868 (Pubitemid 28124064)
14. K. Rothkamm, and M. Lobrich Evidence for a lack of DNA double-strand break repair in human cells exposed to very low x-ray doses Proc. Natl. Acad. Sci. U S A 100 2003 5057 5062 (Pubitemid 36542657)
15. A. Sak, S. Grehl, P. Erichsen, M. Engelhard, A. Grannass, S. Levegrün, C. Pöttgen, M. Groneberg, and M. Stuschke gamma-H2AX foci formation in peripheral blood lymphocytes of tumor patients after local radiotherapy to different sites of the body: dependence on the dose-distribution, irradiated site and time from start of treatment Int. J. Radiat. Biol. 83 2007 639 652 (Pubitemid 47345867)