메뉴 건너뛰기




Volumn 682, Issue , 2011, Pages 249-270

γ-H2AX Detection in Peripheral Blood Lymphocytes, Splenocytes, Bone Marrow, Xenografts, and Skin

Author keywords

Bone marrow; DNA damage; Flow cytometry; Immunoblotting; Immunofluorescence; Lymphocytes; Skin; Splenocytes; Xenografts; H2AX

Indexed keywords

GAMMA H2AX PROTEIN, MOUSE; GAMMA-H2AX PROTEIN, MOUSE; H2AFX PROTEIN, HUMAN; HISTONE;

EID: 79952201940     PISSN: 10643745     EISSN: 19406029     Source Type: Book Series    
DOI: 10.1007/978-1-60327-409-8_18     Document Type: Chapter
Times cited : (69)

References (20)
  • 2
    • 0032489520 scopus 로고    scopus 로고
    • DNA double-stranded breaks induce histone H2AX phosphorylation on serine 139
    • Rogakou, E. P., Pilch, D. R., Orr, A. H., Ivanova, V. S., and Bonner, W. M. (1998) DNA double-stranded breaks induce histone H2AX phosphorylation on serine 139, J. Biol. Chem. 273, 5858–5868.
    • (1998) J. Biol. Chem. , vol.273 , pp. 5858-5868
    • Rogakou, E.P.1    Pilch, D.R.2    Orr, A.H.3    Ivanova, V.S.4    Bonner, W.M.5
  • 3
    • 0032861343 scopus 로고    scopus 로고
    • Megabase chromatin domains involved in DNA double-strand breaks in vivo
    • Rogakou, E. P., Boon, C., Redon, C., and Bonner, W. M. (1999) Megabase chromatin domains involved in DNA double-strand breaks in vivo, J. Cell. Biol. 146, 905–916.
    • (1999) J. Cell. Biol. , vol.146 , pp. 905-916
    • Rogakou, E.P.1    Boon, C.2    Redon, C.3    Bonner, W.M.4
  • 4
    • 0036789173 scopus 로고    scopus 로고
    • Quantitative detection of (125)IdU-induced DNA double-strand breaks with gamma-H2AX antibody
    • Sedelnikova, O. A., Rogakou, E. P., Panyutin, I. G., and Bonner, W. M. (2002) Quantitative detection of (125)IdU-induced DNA double-strand breaks with gamma-H2AX antibody, Radiat. Res. 158, 486–492.
    • (2002) Radiat. Res. , vol.158 , pp. 486-492
    • Sedelnikova, O.A.1    Rogakou, E.P.2    Panyutin, I.G.3    Bonner, W.M.4
  • 5
    • 0037884963 scopus 로고    scopus 로고
    • Evidence for a lack of DNA double-strand break repair in human cells exposed to very low X-ray doses
    • Rothkamm, K., and Lobrich, M. (2003) Evidence for a lack of DNA double-strand break repair in human cells exposed to very low X-ray doses, Proc. Natl. Acad. Sci. U. S. A. 100, 5057–5062.
    • (2003) Proc. Natl. Acad. Sci. U. S. A. , vol.100 , pp. 5057-5062
    • Rothkamm, K.1    Lobrich, M.2
  • 6
    • 0343280013 scopus 로고    scopus 로고
    • A critical role for histone H2AX in recruitment of repair factors to nuclear foci after DNA damage
    • Paull, T. T., Rogakou, E. P., Yamazaki, V., Kirchgessner, C. U., Gellert, M., and Bonner, W. M. (2000) A critical role for histone H2AX in recruitment of repair factors to nuclear foci after DNA damage, Curr. Biol. 10, 886–895.
    • (2000) Curr. Biol. , vol.10 , pp. 886-895
    • Paull, T.T.1    Rogakou, E.P.2    Yamazaki, V.3    Kirchgessner, C.U.4    Gellert, M.5    Bonner, W.M.6
  • 7
    • 28444483130 scopus 로고    scopus 로고
    • Gamma-H2AX dephos-phorylation by protein phosphatase 2A facilitates DNA double-strand break repair
    • Chowdhury, D., Keogh, M. C., Ishii, H., Peterson, C. L., Buratowski, S., and Lieberman, J. (2005) Gamma-H2AX dephos-phorylation by protein phosphatase 2A facilitates DNA double-strand break repair, Mol. Cell. 20, 801–809.
    • (2005) Mol. Cell. , vol.20 , pp. 801-809
    • Chowdhury, D.1    Keogh, M.C.2    Ishii, H.3    Peterson, C.L.4    Buratowski, S.5    Lieberman, J.6
  • 8
    • 0025970972 scopus 로고
    • Principles and practice of DNA filter elution
    • Kohn, K. W. (1991) Principles and practice of DNA filter elution, Pharmacol. Ther. 49, 55–77.
    • (1991) Pharmacol. Ther. , vol.49 , pp. 55-77
    • Kohn, K.W.1
  • 9
    • 34250654965 scopus 로고    scopus 로고
    • The comet assay: A method to measure DNA damage in individual cells
    • Olive, P. L., and Banath, J. P. (2006) The comet assay: a method to measure DNA damage in individual cells, Nat. Protoc. 1, 23–29.
    • (2006) Nat. Protoc. , vol.1 , pp. 23-29
    • Olive, P.L.1    Banath, J.P.2
  • 10
    • 4944234150 scopus 로고    scopus 로고
    • Radiation sensitivity, H2AX phosphorylation, and kinetics of repair of DNA strand breaks in irradiated cervical cancer cell lines
    • Banath, J. P., Macphail, S. H., and Olive, P. L. (2004) Radiation sensitivity, H2AX phosphorylation, and kinetics of repair of DNA strand breaks in irradiated cervical cancer cell lines, Cancer Res. 64, 7144–7149.
    • (2004) Cancer Res , vol.64 , pp. 7144-7149
    • Banath, J.P.1    Macphail, S.H.2    Olive, P.L.3
  • 11
    • 0030877998 scopus 로고    scopus 로고
    • SYBR Green I staining of pulsed field agarose gels is a sensitive and inexpensive way of quantitating DNA double-strand breaks in mammalian cells
    • Kiltie, A. E., and Ryan, A. J. (1997) SYBR Green I staining of pulsed field agarose gels is a sensitive and inexpensive way of quantitating DNA double-strand breaks in mammalian cells, Nucleic Acids Res. 25, 2945–2946.
    • (1997) Nucleic Acids Res , vol.25 , pp. 2945-2946
    • Kiltie, A.E.1    Ryan, A.J.2
  • 12
    • 62949181960 scopus 로고    scopus 로고
    • Gamma-H2AX as a biomarker of DNA damage induced by ionizing radiation in human peripheral blood lymphocytes and artificial skin
    • Redon, C. E., Dickey, J. S., Bonner, W. M., and Sedelnikova, O. A. (2009) Gamma-H2AX as a biomarker of DNA damage induced by ionizing radiation in human peripheral blood lymphocytes and artificial skin, Adv. Space Res. 43, 1171–1178.
    • (2009) Adv. Space Res. , vol.43 , pp. 1171-1178
    • Redon, C.E.1    Dickey, J.S.2    Bonner, W.M.3    Sedelnikova, O.A.4
  • 13
    • 1642575967 scopus 로고    scopus 로고
    • Two-dimensional gel ana.lysis of histones and other H2AX-related methods
    • Pilch, D. R., Redon, C., Sedelnikova, O. A., and Bonner, W. M. (2004) Two-dimensional gel ana.lysis of histones and other H2AX-related methods, Methods Enzymol. 375, 76–88.
    • (2004) Methods Enzymol , vol.375 , pp. 76-88
    • Pilch, D.R.1    Redon, C.2    Sedelnikova, O.A.3    Bonner, W.M.4
  • 15
    • 34247135323 scopus 로고    scopus 로고
    • An optimized method for detecting gamma-H2AX in blood cells reveals a significant interindividual variation in the gamma-H2AX response among humans
    • Ismail, I. H., Wadhra, T. I., and Hammarsten, O. (2007) An optimized method for detecting gamma-H2AX in blood cells reveals a significant interindividual variation in the gamma-H2AX response among humans, Nucleic Acids Res. 35, e36.
    • (2007) Nucleic Acids Res , vol.35 , pp. e36
    • Ismail, I.H.1    Wadhra, T.I.2    Hammarsten, O.3
  • 16
    • 0942278942 scopus 로고    scopus 로고
    • Phosphorylation of histone H2AX as a measure of radiosensitivity
    • Olive, P. L., and Banath, J. P. (2004) Phosphorylation of histone H2AX as a measure of radiosensitivity, Int. J. Radiat. Oncol. Biol. Phys. 58, 331–335.
    • (2004) Int. J. Radiat. Oncol. Biol. Phys. , vol.58 , pp. 331-335
    • Olive, P.L.1    Banath, J.P.2
  • 17
    • 40249086612 scopus 로고    scopus 로고
    • Residual gammaH2AX after irradiation of human lymphocytes and monocytes in vitro and its relation to late effects after prostate brachytherapy
    • Olive, P. L., Banath, J. P., and Keyes, M. (2008) Residual gammaH2AX after irradiation of human lymphocytes and monocytes in vitro and its relation to late effects after prostate brachytherapy, Radiother. Oncol. 86, 336–346.
    • (2008) Radiother. Oncol. , vol.86 , pp. 336-346
    • Olive, P.L.1    Banath, J.P.2    Keyes, M.3
  • 18
    • 33845651212 scopus 로고    scopus 로고
    • GammaH2AX in cancer cells: A potential biomarker for cancer diagnostics, prediction and recurrence
    • Sedelnikova, O. A., and Bonner, W. M. (2006) GammaH2AX in cancer cells: a potential biomarker for cancer diagnostics, prediction and recurrence, Cell Cycle 5, 2909–2913.
    • (2006) Cell Cycle , vol.5 , pp. 2909-2913
    • Sedelnikova, O.A.1    Bonner, W.M.2
  • 19
    • 33846227461 scopus 로고    scopus 로고
    • Constitutive histone H2AX phosphorylation and ATM activation are strongly amplified during mitogenic stimulation of lymphocytes
    • Tanaka, T., Kajstura, M., Halicka, H. D., Traganos, F., and Darzynkiewicz, Z. (2007) Constitutive histone H2AX phosphorylation and ATM activation are strongly amplified during mitogenic stimulation of lymphocytes, Cell Prolif. 40, 1–13.
    • (2007) Cell Prolif , vol.40 , pp. 1-13
    • Tanaka, T.1    Kajstura, M.2    Halicka, H.D.3    Traganos, F.4    Darzynkiewicz, Z.5


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.