γ-H2AX as protein biomarker for radiation exposure

Annali dell'Istituto Superiore di Sanita

Volumn 45, Issue 3, 2009, Pages 265-271

  Rothkamm, Kai ^a   Horn, Simon ^a  

^a PUBLIC HEALTH ENGLAND   (United Kingdom)

Author keywords

h2ax; Biological dosimetry; Double stranded dna break; Radiation accident; Triage

Indexed keywords

BIOLOGICAL MARKER; H2AFX PROTEIN, HUMAN; HISTONE;

ARTICLE; BLOOD; DOUBLE STRANDED DNA BREAK; FLOW CYTOMETRY; FLUORESCENCE MICROSCOPY; HUMAN; PHOSPHORYLATION; PHYSIOLOGY; RADIATION EXPOSURE; RADIATION INJURY;

BIOLOGICAL MARKERS; DNA BREAKS, DOUBLE-STRANDED; FLOW CYTOMETRY; HISTONES; HUMANS; MICROSCOPY, FLUORESCENCE; PHOSPHORYLATION; RADIATION INJURIES;

EID: 70350304614     PISSN: 00212571     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (43)

1. Wyman C, Kanaar R. DNA double-strand break repair: all's well that ends well. Annu Rev Genet 2006;40:363-83. (Pubitemid 44956790)
2. Rothkamm K. Different means to an end: DNA doublestrand break repair. In: Kiefer J (Ed.). Life sciences and radiation. Berlin: Springer Verlag; 2004. p. 179-86.
3. Rodrigue A, Lafrance M, Gauthier MC, McDonald D, Hendzel M, West SC, Jasin M, Masson JY. Interplay between human DNA repair proteins at a unique double-strand break in vivo. EMBO J 2006;25:222-31.
4. Rogakou EP, Pilch DR, Orr AH, Ivanova VS, Bonner WM. DNA double-stranded breaks induce histone H2AX phosphorylation on serine 139. J Biol Chem 1998;273:5858-68.
5. Rogakou E, Boon C, Redon C, Bonner W. Megabase chromatin domains involved in DNA double-strand breaks in vivo. J Cell Biol 1999;146:905-16.
6. Wang H, Wang M, Wang H, Böcker W, Iliakis G. Complex H2AX phosphorylation patterns by multiple kinases including ATM and DNA-PK in human cells exposed to ionizing radiation and treated with kinase inhibitors. J Cell Physiol 2005;202:492-502.
7. Stiff T, O' Driscoll M, Rief N, Iwabuchi K, Lobrich M, Jeggo PA. ATM and DNA-PK function redundantly to phosphorylate H2AX after exposure to ionizing radiation. Cancer Res 2004;64:2390-6.
8. Furuta T, Takemura H, Liao ZY, Aune GJ, Redon C, Sedelnikova OA, Pilch DR, Rogakou EP, Celeste A, Chen HT, Nussenzweig A, Aladjem MI, Bonner WM, Pommier Y. Phosphorylation of histone H2AX and activation of Mre11, Rad50, and Nbs1 in response to replication-dependent DNA double-strand breaks induced by mammalian DNA topoisomerase I cleavage complexes. J Biol Chem 2003;278:20303-12.
9. Rothkamm K, Löbrich M. Evidence for a lack of DNA double-strand break repair in human cells exposed to very low x-ray doses. Proc Natl Acad Sci USA 2003;100:5057-62.
10. Sedelnikova OA, Rogakou EP, Panyutin IG, Bonner WM. Quantitative detection of (125)IdU-induced DNA doublestrand breaks with gamma-H2AX antibody. Radiat Res 2002;158:486-92.
11. Rothkamm K, Krüger I, Thompson LH, Löbrich M. Pathways of DNA double-strand break repair during the mammalian cell cycle. Mol Cell Biol 2003;23:5706-15.
12. Kühne M, Riballo E, Rief N, Rothkamm K, Jeggo PA, Löbrich M. A double-strand break repair defect in ATMdeficient cells contributes to radiosensitivity. Cancer Res 2004;64:500-8.
13. Li S, Takeda Y, Wragg S, Barrett J, Phillips A, Dynan WS. Modification of the ionizing radiation response in living cells by an scFv against the DNA-dependent protein kinase. Nucleic Acids Res 2003;31:5848-57.
14. Takahashi A, Ohnishi T. Does gammaH2AX foci formation depend on the presence of DNA double strand breaks? Cancer Lett 2005;229:171-9.
15. Celeste A, Petersen S, Romanienko P, Fernandez-Capetillo O, Chen H, Sedelnikova O, Reina-San-Martin B, Coppola V, Meffre E, Difilippantonio M, Redon C, Pilch D, Olaru A, Eckhaus M, Camerini-Otero R, Tessarollo L, Livak F, Manova K, Bonner W, Nussenzweig M et al. Genomic instability in mice lacking histone H2AX. Science 2002;296:922-7.
16. Bassing CH, Suh H, Ferguson DO, Chua KF, Manis J, Eckersdorff M, Gleason M, Bronson R, Lee C, Alt FW. Histone H2AX: a dosage-dependent suppressor of oncogenic translocations and tumors. Cell 2003;114:359-70.
17. Kinner A, Wu W, Staudt C, Iliakis G. Gamma-H2AX in recognition and signaling of DNA double-strand breaks in the context of chromatin. Nucleic Acids Res 2008;36:5678-94.
18. Fernandez-Capetillo O, Chen HT, Celeste A, Ward I, Romanienko PJ, Morales JC, Naka K, Xia Z, Camerini-Otero RD, Motoyama N, Carpenter PB, Bonner WM, Chen J, Nussenzweig A. DNA damage-induced G2-M checkpoint activation by histone H2AX and 53BP1. Nat Cell Biol 2002;4:993-7.
19. Riballo E, Kühne M, Rief N, Doherty A, Smith GC, Recio MJ, Reis C, Dahm K, Fricke A, Krempler A, Parker AR, Jackson SP, Gennery A, Jeggo PA, Löbrich M. A pathway of double-strand break rejoining dependent upon ATM, Artemis, and proteins locating to gamma-H2AX foci. Mol Cell 2004;16:715-24.
20. Lowndes NF, Toh GW. DNA Repair: The Importance of Phosphorylating Histone H2AX. Curr Biol 2005;15:R99-R102.
21. van Attikum H, Gasser SM. The histone code at DNA breaks: a guide to repair? Nat Rev Mol Cell Biol 2005;6:757-65.
22. Fernandez-Capetillo O, Lee A, Nussenzweig M, Nussenzweig A. H2AX: the histone guardian of the genome. DNA Repair (Amst) 2004;3:959-67.
23. Bassing CH, Alt FW. H2AX May Function as an Anchor to Hold Broken Chromosomal DNA Ends in Close Proximity. Cell Cycle 2004;3:149-53.
24. Dhillon VS, Thomas P, Fenech M. Comparison of DNA damage and repair following radiation challenge in buccal cells and lymphocytes using single-cell gel electrophoresis. Int J Radiat Biol 2004;80:517-28.
25. International Atomic Energy Agency. Cytogenetic analysis for radiation dose assessment. Vienna: IAEA; 2001. (Technical Report Series, No 405).
26. Rothkamm K, Balroop S, Shekhdar J, Fernie P, Goh V.Leukocyte DNA damage after multi-detector row CT: a quantitative biomarker of low-level radiation exposure. Radiology 2007;242:244-51.
27. Sak A, Grehl S, Erichsen P, Engelhard M, Grannass A, Levegrun S, Pottgen C, Groneberg M, Stuschke M. Gamma-H2AX foci formation in peripheral blood lymphocytes of tumor patients after local radiotherapy to different sites of the body: dependence on the dose-distribution, irradiated site and time from start of treatment. Int J Radiat Biol 2007;83:639-52.
28. Löbrich M, Rief N, Khne M, Heckmann M, Fleckenstein J, Rübe C, Uder M. In vivo formation and repair of DNA double-strand breaks after computed tomography examinations. Proc Natl Acad Sci USA 2005;102:8984-9.
29. Geisel D, Heverhagen JT, Kalinowski M, Wagner H. DNA double-strand breaks after percutaneous transluminal angioplasty. Radiology 2008;248:852-9.
30. Kuefner MA, Grudzenski S, Schwab SA, Azoulay S, Heckmann M, Heinrich MC, Lobrich M, Uder M. X-ray-induced DNA double-strand breaks after angiographic examinations of different anatomic regions. Rofo 2009;181:374-80.
31. Costes SV, Boissiere A, Ravani S, Romano R, Parvin B, Barcellos-Hoff MH. Imaging features that discriminate between foci induced by high- and low-LET radiation in human fibroblasts. Radiat Res 2006;165:505-15.
32. Qvarnstrom OF, Simonsson M, Johansson KA, Nyman J, Turesson I. DNA double strand break quantification in skin biopsies. Radiother Oncol 2004;72:311-7.
33. Carpenter AE, Jones TR, Lamprecht MR, Clarke C, Kang IH, Friman O, Guertin DA, Chang JH, Lindquist RA, Moffat J, Golland P, Sabatini DM. CellProfiler: image analysis software for identifying and quantifying cell phenotypes. Genome Biol 2006;7:R100.
34. Bocker W, Iliakis G. Computational Methods for analysis of foci: validation for radiation-induced gamma-H2AX foci in human cells. Radiat Res 2006;165:113-24.
35. Barber PR, Locke RJ, Pierce GP, Rothkamm K, Vojnovic B. Gamma-H2AX foci counting: image processing and control software for high content screening. Proc SPIE 2007;6441: M1-M10.
36. Huang X, Halicka HD, Traganos F, Tanaka T, Kurose A, Darzynkiewicz Z. Cytometric assessment of DNA damage in relation to cell cycle phase and apoptosis. Cell Prolif 2005;38:223-43.
37. Ismail IH, Wadhra TI, Hammarsten O. An optimized method for detecting gamma-H2AX in blood cells reveals a significant interindividual variation in the gamma-H2AX response among humans. Nucleic Acids Res 2007;35:e36.
38. Taneja N, Davis M, Choy JS, Beckett MA, Singh R, Kron SJ, Weichselbaum RR. Histone H2AX phosphorylation as a predictor of radiosensitivity and target for radiotherapy. J Biol Chem 2004;279:2273-80.
39. Albino AP, Huang X, Jorgensen E, Yang J, Gietl D, Traganos F, Darzynkiewicz Z. Induction of H2AX Phosphorylation in Pulmonary Cells by Tobacco Smoke: A New Assay for Carcinogens. Cell Cycle 2004;3:1062-8.
40. Kato T, Nagasawa H, Warner C, Okayasu R, Bedford JS. Cytotoxicity of cigarette smoke condensate is not due to DNA double strand breaks: Comparative studies using radiosensitive mutant and wild-type CHO cells. Int J Radiat Biol 2007;83:583-91.
41. Winslow JE, Hinshaw JW, Hughes MJ, Williams RC, Bozeman WP. Quantitative assessment of diagnostic radiation doses in adult blunt trauma patients. Ann Emerg Med 2008;52:93-7.
42. Ishizaki K, Hayashi Y, Nakamura H, Yasui Y, Komatsu K, Tachibana A. No induction of p53 phosphorylation and few focus formation of phosphorylated H2AX suggest efficient repair of DNA damage during chronic low-dose-rate irradiation in human cells. J Radiat Res (Tokyo) 2004;45:521-5.
43. Blakely WF, Salter CA, Prasanna PGS. Early-response biological dosimetry-recommended countermeasure enhancements for mass-casualty radiological incidents and terrorism. Health Phys 2005;89:494-504.